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Gene Polymorphisms of Micrornas in Helicobacter pylori-Induced High Risk Atrophic Gastritis and Gastric Cancer

BACKGROUND AND AIMS: MicroRNAs (miRNAs) are known for their function as translational regulators of tumor suppressor or oncogenes. Single nucleotide polymorphisms (SNPs) in miRNAs related genes have been shown to affect the regulatory capacity of miRNAs and were linked with gastric cancer (GC) and p...

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Autores principales: Kupcinskas, Juozas, Wex, Thomas, Link, Alexander, Leja, Marcis, Bruzaite, Indre, Steponaitiene, Ruta, Juzenas, Simonas, Gyvyte, Ugne, Ivanauskas, Audrius, Ancans, Guntis, Petrenkiene, Vitalija, Skieceviciene, Jurgita, Kupcinskas, Limas, Malfertheiner, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903675/
https://www.ncbi.nlm.nih.gov/pubmed/24475294
http://dx.doi.org/10.1371/journal.pone.0087467
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author Kupcinskas, Juozas
Wex, Thomas
Link, Alexander
Leja, Marcis
Bruzaite, Indre
Steponaitiene, Ruta
Juzenas, Simonas
Gyvyte, Ugne
Ivanauskas, Audrius
Ancans, Guntis
Petrenkiene, Vitalija
Skieceviciene, Jurgita
Kupcinskas, Limas
Malfertheiner, Peter
author_facet Kupcinskas, Juozas
Wex, Thomas
Link, Alexander
Leja, Marcis
Bruzaite, Indre
Steponaitiene, Ruta
Juzenas, Simonas
Gyvyte, Ugne
Ivanauskas, Audrius
Ancans, Guntis
Petrenkiene, Vitalija
Skieceviciene, Jurgita
Kupcinskas, Limas
Malfertheiner, Peter
author_sort Kupcinskas, Juozas
collection PubMed
description BACKGROUND AND AIMS: MicroRNAs (miRNAs) are known for their function as translational regulators of tumor suppressor or oncogenes. Single nucleotide polymorphisms (SNPs) in miRNAs related genes have been shown to affect the regulatory capacity of miRNAs and were linked with gastric cancer (GC) and premalignant gastric conditions. The purpose of this study was to evaluate potential associations between miRNA-related gene polymorphisms (miR-27a, miR-146a, miR-196a-2, miR-492 and miR-608) and the presence of GC or high risk atrophic gastritis (HRAG) in European population. METHODS: Gene polymorphisms were analyzed in 995 subjects (controls: n = 351; GC: n = 363; HRAG: n = 281) of European descent. MiR-27a T>C (rs895819), miR-146a G>C (rs2910164), miR-196a-2 C>T (rs11614913), miR-492 G>C (rs2289030) and miR-608 C>G (rs4919510) SNPs were genotyped by RT-PCR. RESULTS: Overall, SNPs of miRNAs were not associated with the presence of GC or HRAG. We observed a tendency for miR-196a-2 CT genotype to be associated with higher risk of GC when compared to CC genotype, however, the difference did not reach the adjusted P-value (odds ratio (OR) - 1.46, 95% confidence interval (CI) 1.03-2.07, P = 0.032). MiR-608 GG genotype was more frequent in GC when compared to controls (OR −2.34, 95% CI 1.08–5.04), but significance remained marginal (P = 0.029). A similar tendency was observed in a recessive model for miR-608, where CC + CG vs GG genotype comparison showed a tendency for increased risk of GC with OR of 2.44 (95% CI 1.14–5.22, P = 0.021). The genotypes and alleles of miR-27a, miR-146a, miR-196a-2, miR-492 and miR-608 SNPs had similar distribution between histological subtypes of GC and were not linked with the presence of diffuse or intestinal-type GC. CONCLUSIONS: Gene polymorphisms of miR-27a, miR-146a, miR-196a-2, miR-492, miR-492a and miR-608 were not associated with the presence of HRAG, GC or different histological subtypes of GC in European subjects.
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spelling pubmed-39036752014-01-28 Gene Polymorphisms of Micrornas in Helicobacter pylori-Induced High Risk Atrophic Gastritis and Gastric Cancer Kupcinskas, Juozas Wex, Thomas Link, Alexander Leja, Marcis Bruzaite, Indre Steponaitiene, Ruta Juzenas, Simonas Gyvyte, Ugne Ivanauskas, Audrius Ancans, Guntis Petrenkiene, Vitalija Skieceviciene, Jurgita Kupcinskas, Limas Malfertheiner, Peter PLoS One Research Article BACKGROUND AND AIMS: MicroRNAs (miRNAs) are known for their function as translational regulators of tumor suppressor or oncogenes. Single nucleotide polymorphisms (SNPs) in miRNAs related genes have been shown to affect the regulatory capacity of miRNAs and were linked with gastric cancer (GC) and premalignant gastric conditions. The purpose of this study was to evaluate potential associations between miRNA-related gene polymorphisms (miR-27a, miR-146a, miR-196a-2, miR-492 and miR-608) and the presence of GC or high risk atrophic gastritis (HRAG) in European population. METHODS: Gene polymorphisms were analyzed in 995 subjects (controls: n = 351; GC: n = 363; HRAG: n = 281) of European descent. MiR-27a T>C (rs895819), miR-146a G>C (rs2910164), miR-196a-2 C>T (rs11614913), miR-492 G>C (rs2289030) and miR-608 C>G (rs4919510) SNPs were genotyped by RT-PCR. RESULTS: Overall, SNPs of miRNAs were not associated with the presence of GC or HRAG. We observed a tendency for miR-196a-2 CT genotype to be associated with higher risk of GC when compared to CC genotype, however, the difference did not reach the adjusted P-value (odds ratio (OR) - 1.46, 95% confidence interval (CI) 1.03-2.07, P = 0.032). MiR-608 GG genotype was more frequent in GC when compared to controls (OR −2.34, 95% CI 1.08–5.04), but significance remained marginal (P = 0.029). A similar tendency was observed in a recessive model for miR-608, where CC + CG vs GG genotype comparison showed a tendency for increased risk of GC with OR of 2.44 (95% CI 1.14–5.22, P = 0.021). The genotypes and alleles of miR-27a, miR-146a, miR-196a-2, miR-492 and miR-608 SNPs had similar distribution between histological subtypes of GC and were not linked with the presence of diffuse or intestinal-type GC. CONCLUSIONS: Gene polymorphisms of miR-27a, miR-146a, miR-196a-2, miR-492, miR-492a and miR-608 were not associated with the presence of HRAG, GC or different histological subtypes of GC in European subjects. Public Library of Science 2014-01-27 /pmc/articles/PMC3903675/ /pubmed/24475294 http://dx.doi.org/10.1371/journal.pone.0087467 Text en © 2014 Kupcinskas et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kupcinskas, Juozas
Wex, Thomas
Link, Alexander
Leja, Marcis
Bruzaite, Indre
Steponaitiene, Ruta
Juzenas, Simonas
Gyvyte, Ugne
Ivanauskas, Audrius
Ancans, Guntis
Petrenkiene, Vitalija
Skieceviciene, Jurgita
Kupcinskas, Limas
Malfertheiner, Peter
Gene Polymorphisms of Micrornas in Helicobacter pylori-Induced High Risk Atrophic Gastritis and Gastric Cancer
title Gene Polymorphisms of Micrornas in Helicobacter pylori-Induced High Risk Atrophic Gastritis and Gastric Cancer
title_full Gene Polymorphisms of Micrornas in Helicobacter pylori-Induced High Risk Atrophic Gastritis and Gastric Cancer
title_fullStr Gene Polymorphisms of Micrornas in Helicobacter pylori-Induced High Risk Atrophic Gastritis and Gastric Cancer
title_full_unstemmed Gene Polymorphisms of Micrornas in Helicobacter pylori-Induced High Risk Atrophic Gastritis and Gastric Cancer
title_short Gene Polymorphisms of Micrornas in Helicobacter pylori-Induced High Risk Atrophic Gastritis and Gastric Cancer
title_sort gene polymorphisms of micrornas in helicobacter pylori-induced high risk atrophic gastritis and gastric cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903675/
https://www.ncbi.nlm.nih.gov/pubmed/24475294
http://dx.doi.org/10.1371/journal.pone.0087467
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