Cargando…

Molecular Characterization of Clostridium botulinum Isolates from Foodborne Outbreaks in Thailand, 2010

BACKGROUND: Thailand has had several foodborne outbreaks of botulism, one of the biggest being in 2006 when laboratory investigations identified the etiologic agent as Clostridium botulinum type A. Identification of the etiologic agent from outbreak samples is laborious using conventional microbiolo...

Descripción completa

Detalles Bibliográficos
Autores principales: Wangroongsarb, Piyada, Kohda, Tomoko, Jittaprasartsin, Chutima, Suthivarakom, Karun, Kamthalang, Thanitchi, Umeda, Kaoru, Sawanpanyalert, Pathom, Kozaki, Shunji, Ikuta, Kazuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903786/
https://www.ncbi.nlm.nih.gov/pubmed/24475015
http://dx.doi.org/10.1371/journal.pone.0077792
_version_ 1782301142770778112
author Wangroongsarb, Piyada
Kohda, Tomoko
Jittaprasartsin, Chutima
Suthivarakom, Karun
Kamthalang, Thanitchi
Umeda, Kaoru
Sawanpanyalert, Pathom
Kozaki, Shunji
Ikuta, Kazuyoshi
author_facet Wangroongsarb, Piyada
Kohda, Tomoko
Jittaprasartsin, Chutima
Suthivarakom, Karun
Kamthalang, Thanitchi
Umeda, Kaoru
Sawanpanyalert, Pathom
Kozaki, Shunji
Ikuta, Kazuyoshi
author_sort Wangroongsarb, Piyada
collection PubMed
description BACKGROUND: Thailand has had several foodborne outbreaks of botulism, one of the biggest being in 2006 when laboratory investigations identified the etiologic agent as Clostridium botulinum type A. Identification of the etiologic agent from outbreak samples is laborious using conventional microbiological methods and the neurotoxin mouse bioassay. Advances in molecular techniques have added enormous information regarding the etiology of outbreaks and characterization of isolates. We applied these methods in three outbreaks of botulism in Thailand in 2010. METHODOLOGY/PRINCIPAL FINDINGS: A total of 19 cases were involved (seven each in Lampang and Saraburi and five in Maehongson provinces). The first outbreak in Lampang province in April 2010 was associated with C. botulinum type F, which was detected by conventional methods. Outbreaks in Saraburi and Maehongson provinces occurred in May and December were due to C. botulinum type A1(B) and B that were identified by conventional methods and molecular techniques, respectively. The result of phylogenetic sequence analysis showed that C. botulinum type A1(B) strain Saraburi 2010 was close to strain Iwate 2007. Molecular analysis of the third outbreak in Maehongson province showed C. botulinum type B8, which was different from B1–B7 subtype. The nontoxic component genes of strain Maehongson 2010 revealed that ha33, ha17 and botR genes were close to strain Okra (B1) while ha70 and ntnh genes were close to strain 111 (B2). CONCLUSION/SIGNIFICANCE: This study demonstrates the utility of molecular genotyping of C. botulinum and how it contributes to our understanding the epidemiology and variation of boNT gene. Thus, the recent botulism outbreaks in Thailand were induced by various C. botulinum types.
format Online
Article
Text
id pubmed-3903786
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-39037862014-01-28 Molecular Characterization of Clostridium botulinum Isolates from Foodborne Outbreaks in Thailand, 2010 Wangroongsarb, Piyada Kohda, Tomoko Jittaprasartsin, Chutima Suthivarakom, Karun Kamthalang, Thanitchi Umeda, Kaoru Sawanpanyalert, Pathom Kozaki, Shunji Ikuta, Kazuyoshi PLoS One Research Article BACKGROUND: Thailand has had several foodborne outbreaks of botulism, one of the biggest being in 2006 when laboratory investigations identified the etiologic agent as Clostridium botulinum type A. Identification of the etiologic agent from outbreak samples is laborious using conventional microbiological methods and the neurotoxin mouse bioassay. Advances in molecular techniques have added enormous information regarding the etiology of outbreaks and characterization of isolates. We applied these methods in three outbreaks of botulism in Thailand in 2010. METHODOLOGY/PRINCIPAL FINDINGS: A total of 19 cases were involved (seven each in Lampang and Saraburi and five in Maehongson provinces). The first outbreak in Lampang province in April 2010 was associated with C. botulinum type F, which was detected by conventional methods. Outbreaks in Saraburi and Maehongson provinces occurred in May and December were due to C. botulinum type A1(B) and B that were identified by conventional methods and molecular techniques, respectively. The result of phylogenetic sequence analysis showed that C. botulinum type A1(B) strain Saraburi 2010 was close to strain Iwate 2007. Molecular analysis of the third outbreak in Maehongson province showed C. botulinum type B8, which was different from B1–B7 subtype. The nontoxic component genes of strain Maehongson 2010 revealed that ha33, ha17 and botR genes were close to strain Okra (B1) while ha70 and ntnh genes were close to strain 111 (B2). CONCLUSION/SIGNIFICANCE: This study demonstrates the utility of molecular genotyping of C. botulinum and how it contributes to our understanding the epidemiology and variation of boNT gene. Thus, the recent botulism outbreaks in Thailand were induced by various C. botulinum types. Public Library of Science 2014-01-27 /pmc/articles/PMC3903786/ /pubmed/24475015 http://dx.doi.org/10.1371/journal.pone.0077792 Text en © 2014 Wangroongsarb et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wangroongsarb, Piyada
Kohda, Tomoko
Jittaprasartsin, Chutima
Suthivarakom, Karun
Kamthalang, Thanitchi
Umeda, Kaoru
Sawanpanyalert, Pathom
Kozaki, Shunji
Ikuta, Kazuyoshi
Molecular Characterization of Clostridium botulinum Isolates from Foodborne Outbreaks in Thailand, 2010
title Molecular Characterization of Clostridium botulinum Isolates from Foodborne Outbreaks in Thailand, 2010
title_full Molecular Characterization of Clostridium botulinum Isolates from Foodborne Outbreaks in Thailand, 2010
title_fullStr Molecular Characterization of Clostridium botulinum Isolates from Foodborne Outbreaks in Thailand, 2010
title_full_unstemmed Molecular Characterization of Clostridium botulinum Isolates from Foodborne Outbreaks in Thailand, 2010
title_short Molecular Characterization of Clostridium botulinum Isolates from Foodborne Outbreaks in Thailand, 2010
title_sort molecular characterization of clostridium botulinum isolates from foodborne outbreaks in thailand, 2010
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3903786/
https://www.ncbi.nlm.nih.gov/pubmed/24475015
http://dx.doi.org/10.1371/journal.pone.0077792
work_keys_str_mv AT wangroongsarbpiyada molecularcharacterizationofclostridiumbotulinumisolatesfromfoodborneoutbreaksinthailand2010
AT kohdatomoko molecularcharacterizationofclostridiumbotulinumisolatesfromfoodborneoutbreaksinthailand2010
AT jittaprasartsinchutima molecularcharacterizationofclostridiumbotulinumisolatesfromfoodborneoutbreaksinthailand2010
AT suthivarakomkarun molecularcharacterizationofclostridiumbotulinumisolatesfromfoodborneoutbreaksinthailand2010
AT kamthalangthanitchi molecularcharacterizationofclostridiumbotulinumisolatesfromfoodborneoutbreaksinthailand2010
AT umedakaoru molecularcharacterizationofclostridiumbotulinumisolatesfromfoodborneoutbreaksinthailand2010
AT sawanpanyalertpathom molecularcharacterizationofclostridiumbotulinumisolatesfromfoodborneoutbreaksinthailand2010
AT kozakishunji molecularcharacterizationofclostridiumbotulinumisolatesfromfoodborneoutbreaksinthailand2010
AT ikutakazuyoshi molecularcharacterizationofclostridiumbotulinumisolatesfromfoodborneoutbreaksinthailand2010