A new method for maturity-dependent fractionation of neutrophil progenitors applicable for the study of myelodysplastic syndromes

We applied our new method, maturity-dependent fractionation of bone marrow-derived neutrophil progenitors, to a study of gene expression profiles during granulopoiesis in myelodysplastic syndromes. CD34(+) cells with low density [F1], CD11b(-)/CD16(-) [F2], CD11b(+)/CD16(-) [F3] and CD11b(+)/CD16(lo...

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Autores principales: Hu, Huiyuan, Shikama, Yayoi, Shichishima, Tsutomu, Ikeda, Kazuhiko, Akutsu, Kazuko, Ono, Tomoyuki, Kimura, Hideo, Ogawa, Kazuei, Noji, Hideyoshi, Takeishi, Yasuchika, Kimura, Junko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
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Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3904161/
https://www.ncbi.nlm.nih.gov/pubmed/24451620
http://dx.doi.org/10.1186/2050-7771-2-2
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author Hu, Huiyuan
Shikama, Yayoi
Shichishima, Tsutomu
Ikeda, Kazuhiko
Akutsu, Kazuko
Ono, Tomoyuki
Kimura, Hideo
Ogawa, Kazuei
Noji, Hideyoshi
Takeishi, Yasuchika
Kimura, Junko
author_facet Hu, Huiyuan
Shikama, Yayoi
Shichishima, Tsutomu
Ikeda, Kazuhiko
Akutsu, Kazuko
Ono, Tomoyuki
Kimura, Hideo
Ogawa, Kazuei
Noji, Hideyoshi
Takeishi, Yasuchika
Kimura, Junko
author_sort Hu, Huiyuan
collection PubMed
description We applied our new method, maturity-dependent fractionation of bone marrow-derived neutrophil progenitors, to a study of gene expression profiles during granulopoiesis in myelodysplastic syndromes. CD34(+) cells with low density [F1], CD11b(-)/CD16(-) [F2], CD11b(+)/CD16(-) [F3] and CD11b(+)/CD16(low) [F4] with intermediate density, CD11b(+)/CD16(int) [F5] and CD11b(+)/CD16(high) [F6] with high density were isolated from six patients. Although AML1 and C/EBP-ϵ mRNA peaked at F1 and F4, respectively, in healthy individuals, C/EBP-ϵ was maximized at F2/F3 in all patients, two of whom showed simultaneous peaks of AML1 at F2. Thus, this fractionation is useful to detect mistimed induction of granulopoiesis-regulating genes in myelodysplastic syndromes.
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spelling pubmed-39041612014-01-29 A new method for maturity-dependent fractionation of neutrophil progenitors applicable for the study of myelodysplastic syndromes Hu, Huiyuan Shikama, Yayoi Shichishima, Tsutomu Ikeda, Kazuhiko Akutsu, Kazuko Ono, Tomoyuki Kimura, Hideo Ogawa, Kazuei Noji, Hideyoshi Takeishi, Yasuchika Kimura, Junko Biomark Res Letter to the Editor We applied our new method, maturity-dependent fractionation of bone marrow-derived neutrophil progenitors, to a study of gene expression profiles during granulopoiesis in myelodysplastic syndromes. CD34(+) cells with low density [F1], CD11b(-)/CD16(-) [F2], CD11b(+)/CD16(-) [F3] and CD11b(+)/CD16(low) [F4] with intermediate density, CD11b(+)/CD16(int) [F5] and CD11b(+)/CD16(high) [F6] with high density were isolated from six patients. Although AML1 and C/EBP-ϵ mRNA peaked at F1 and F4, respectively, in healthy individuals, C/EBP-ϵ was maximized at F2/F3 in all patients, two of whom showed simultaneous peaks of AML1 at F2. Thus, this fractionation is useful to detect mistimed induction of granulopoiesis-regulating genes in myelodysplastic syndromes. BioMed Central 2014-01-22 /pmc/articles/PMC3904161/ /pubmed/24451620 http://dx.doi.org/10.1186/2050-7771-2-2 Text en Copyright © 2014 Hu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Letter to the Editor
Hu, Huiyuan
Shikama, Yayoi
Shichishima, Tsutomu
Ikeda, Kazuhiko
Akutsu, Kazuko
Ono, Tomoyuki
Kimura, Hideo
Ogawa, Kazuei
Noji, Hideyoshi
Takeishi, Yasuchika
Kimura, Junko
A new method for maturity-dependent fractionation of neutrophil progenitors applicable for the study of myelodysplastic syndromes
title A new method for maturity-dependent fractionation of neutrophil progenitors applicable for the study of myelodysplastic syndromes
title_full A new method for maturity-dependent fractionation of neutrophil progenitors applicable for the study of myelodysplastic syndromes
title_fullStr A new method for maturity-dependent fractionation of neutrophil progenitors applicable for the study of myelodysplastic syndromes
title_full_unstemmed A new method for maturity-dependent fractionation of neutrophil progenitors applicable for the study of myelodysplastic syndromes
title_short A new method for maturity-dependent fractionation of neutrophil progenitors applicable for the study of myelodysplastic syndromes
title_sort new method for maturity-dependent fractionation of neutrophil progenitors applicable for the study of myelodysplastic syndromes
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3904161/
https://www.ncbi.nlm.nih.gov/pubmed/24451620
http://dx.doi.org/10.1186/2050-7771-2-2
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