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Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos
Nitric oxide is a bioactive signalling molecule that is known to affect a wide range of neurodevelopmental processes. However, its functional relevance to neuromuscular development is not fully understood. Here we have examined developmental roles of nitric oxide during formation and maturation of n...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3904980/ https://www.ncbi.nlm.nih.gov/pubmed/24489806 http://dx.doi.org/10.1371/journal.pone.0086930 |
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author | Jay, Michael Bradley, Sophie McDearmid, Jonathan Robert |
author_facet | Jay, Michael Bradley, Sophie McDearmid, Jonathan Robert |
author_sort | Jay, Michael |
collection | PubMed |
description | Nitric oxide is a bioactive signalling molecule that is known to affect a wide range of neurodevelopmental processes. However, its functional relevance to neuromuscular development is not fully understood. Here we have examined developmental roles of nitric oxide during formation and maturation of neuromuscular contacts in zebrafish. Using histochemical approaches we show that elevating nitric oxide levels reduces the number of neuromuscular synapses within the axial swimming muscles whilst inhibition of nitric oxide biosynthesis has the opposite effect. We further show that nitric oxide signalling does not change synapse density, suggesting that the observed effects are a consequence of previously reported changes in motor axon branch formation. Moreover, we have used in vivo patch clamp electrophysiology to examine the effects of nitric oxide on physiological maturation of zebrafish neuromuscular junctions. We show that developmental exposure to nitric oxide affects the kinetics of spontaneous miniature end plate currents and impacts the neuromuscular drive for locomotion. Taken together, our findings implicate nitrergic signalling in the regulation of zebrafish neuromuscular development and locomotor maturation. |
format | Online Article Text |
id | pubmed-3904980 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39049802014-01-31 Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos Jay, Michael Bradley, Sophie McDearmid, Jonathan Robert PLoS One Research Article Nitric oxide is a bioactive signalling molecule that is known to affect a wide range of neurodevelopmental processes. However, its functional relevance to neuromuscular development is not fully understood. Here we have examined developmental roles of nitric oxide during formation and maturation of neuromuscular contacts in zebrafish. Using histochemical approaches we show that elevating nitric oxide levels reduces the number of neuromuscular synapses within the axial swimming muscles whilst inhibition of nitric oxide biosynthesis has the opposite effect. We further show that nitric oxide signalling does not change synapse density, suggesting that the observed effects are a consequence of previously reported changes in motor axon branch formation. Moreover, we have used in vivo patch clamp electrophysiology to examine the effects of nitric oxide on physiological maturation of zebrafish neuromuscular junctions. We show that developmental exposure to nitric oxide affects the kinetics of spontaneous miniature end plate currents and impacts the neuromuscular drive for locomotion. Taken together, our findings implicate nitrergic signalling in the regulation of zebrafish neuromuscular development and locomotor maturation. Public Library of Science 2014-01-28 /pmc/articles/PMC3904980/ /pubmed/24489806 http://dx.doi.org/10.1371/journal.pone.0086930 Text en © 2014 Jay et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Jay, Michael Bradley, Sophie McDearmid, Jonathan Robert Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos |
title | Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos |
title_full | Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos |
title_fullStr | Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos |
title_full_unstemmed | Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos |
title_short | Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos |
title_sort | effects of nitric oxide on neuromuscular properties of developing zebrafish embryos |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3904980/ https://www.ncbi.nlm.nih.gov/pubmed/24489806 http://dx.doi.org/10.1371/journal.pone.0086930 |
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