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Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos

Nitric oxide is a bioactive signalling molecule that is known to affect a wide range of neurodevelopmental processes. However, its functional relevance to neuromuscular development is not fully understood. Here we have examined developmental roles of nitric oxide during formation and maturation of n...

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Detalles Bibliográficos
Autores principales: Jay, Michael, Bradley, Sophie, McDearmid, Jonathan Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3904980/
https://www.ncbi.nlm.nih.gov/pubmed/24489806
http://dx.doi.org/10.1371/journal.pone.0086930
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author Jay, Michael
Bradley, Sophie
McDearmid, Jonathan Robert
author_facet Jay, Michael
Bradley, Sophie
McDearmid, Jonathan Robert
author_sort Jay, Michael
collection PubMed
description Nitric oxide is a bioactive signalling molecule that is known to affect a wide range of neurodevelopmental processes. However, its functional relevance to neuromuscular development is not fully understood. Here we have examined developmental roles of nitric oxide during formation and maturation of neuromuscular contacts in zebrafish. Using histochemical approaches we show that elevating nitric oxide levels reduces the number of neuromuscular synapses within the axial swimming muscles whilst inhibition of nitric oxide biosynthesis has the opposite effect. We further show that nitric oxide signalling does not change synapse density, suggesting that the observed effects are a consequence of previously reported changes in motor axon branch formation. Moreover, we have used in vivo patch clamp electrophysiology to examine the effects of nitric oxide on physiological maturation of zebrafish neuromuscular junctions. We show that developmental exposure to nitric oxide affects the kinetics of spontaneous miniature end plate currents and impacts the neuromuscular drive for locomotion. Taken together, our findings implicate nitrergic signalling in the regulation of zebrafish neuromuscular development and locomotor maturation.
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spelling pubmed-39049802014-01-31 Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos Jay, Michael Bradley, Sophie McDearmid, Jonathan Robert PLoS One Research Article Nitric oxide is a bioactive signalling molecule that is known to affect a wide range of neurodevelopmental processes. However, its functional relevance to neuromuscular development is not fully understood. Here we have examined developmental roles of nitric oxide during formation and maturation of neuromuscular contacts in zebrafish. Using histochemical approaches we show that elevating nitric oxide levels reduces the number of neuromuscular synapses within the axial swimming muscles whilst inhibition of nitric oxide biosynthesis has the opposite effect. We further show that nitric oxide signalling does not change synapse density, suggesting that the observed effects are a consequence of previously reported changes in motor axon branch formation. Moreover, we have used in vivo patch clamp electrophysiology to examine the effects of nitric oxide on physiological maturation of zebrafish neuromuscular junctions. We show that developmental exposure to nitric oxide affects the kinetics of spontaneous miniature end plate currents and impacts the neuromuscular drive for locomotion. Taken together, our findings implicate nitrergic signalling in the regulation of zebrafish neuromuscular development and locomotor maturation. Public Library of Science 2014-01-28 /pmc/articles/PMC3904980/ /pubmed/24489806 http://dx.doi.org/10.1371/journal.pone.0086930 Text en © 2014 Jay et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jay, Michael
Bradley, Sophie
McDearmid, Jonathan Robert
Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos
title Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos
title_full Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos
title_fullStr Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos
title_full_unstemmed Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos
title_short Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos
title_sort effects of nitric oxide on neuromuscular properties of developing zebrafish embryos
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3904980/
https://www.ncbi.nlm.nih.gov/pubmed/24489806
http://dx.doi.org/10.1371/journal.pone.0086930
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