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Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4

The outbreak of diarrhea and hemolytic uremic syndrome that occurred in Germany in 2011 was caused by a Shiga toxin-producing enteroaggregative Escherichia coli (EAEC) strain. The strain was classified as EAEC due to the presence of a plasmid (pAA) that mediates a characteristic pattern of aggregati...

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Autores principales: Munera, Diana, Ritchie, Jennifer M, Hatzios, Stavroula K, Bronson, Rod, Fang, Gang, Schadt, Eric E, Davis, Brigid M, Waldor, Matthew K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905246/
https://www.ncbi.nlm.nih.gov/pubmed/24445323
http://dx.doi.org/10.1038/ncomms4080
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author Munera, Diana
Ritchie, Jennifer M
Hatzios, Stavroula K
Bronson, Rod
Fang, Gang
Schadt, Eric E
Davis, Brigid M
Waldor, Matthew K
author_facet Munera, Diana
Ritchie, Jennifer M
Hatzios, Stavroula K
Bronson, Rod
Fang, Gang
Schadt, Eric E
Davis, Brigid M
Waldor, Matthew K
author_sort Munera, Diana
collection PubMed
description The outbreak of diarrhea and hemolytic uremic syndrome that occurred in Germany in 2011 was caused by a Shiga toxin-producing enteroaggregative Escherichia coli (EAEC) strain. The strain was classified as EAEC due to the presence of a plasmid (pAA) that mediates a characteristic pattern of aggregative adherence on cultured cells, the defining feature of EAEC that has classically been associated with virulence. Here, we describe an infant rabbit-based model of intestinal colonization and diarrhea caused by the outbreak strain, which we use to decipher the factors that mediate the pathogen’s virulence. Shiga toxin is the key factor required for diarrhea. Unexpectedly, we observe that pAA is dispensable for intestinal colonization and development of intestinal pathology. Instead, chromosome-encoded autotransporters are critical for robust colonization and diarrheal disease in this model. Our findings suggest that conventional wisdom linking aggregative adherence to EAEC intestinal colonization is false for at least a subset of strains.
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spelling pubmed-39052462014-07-20 Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4 Munera, Diana Ritchie, Jennifer M Hatzios, Stavroula K Bronson, Rod Fang, Gang Schadt, Eric E Davis, Brigid M Waldor, Matthew K Nat Commun Article The outbreak of diarrhea and hemolytic uremic syndrome that occurred in Germany in 2011 was caused by a Shiga toxin-producing enteroaggregative Escherichia coli (EAEC) strain. The strain was classified as EAEC due to the presence of a plasmid (pAA) that mediates a characteristic pattern of aggregative adherence on cultured cells, the defining feature of EAEC that has classically been associated with virulence. Here, we describe an infant rabbit-based model of intestinal colonization and diarrhea caused by the outbreak strain, which we use to decipher the factors that mediate the pathogen’s virulence. Shiga toxin is the key factor required for diarrhea. Unexpectedly, we observe that pAA is dispensable for intestinal colonization and development of intestinal pathology. Instead, chromosome-encoded autotransporters are critical for robust colonization and diarrheal disease in this model. Our findings suggest that conventional wisdom linking aggregative adherence to EAEC intestinal colonization is false for at least a subset of strains. 2014 /pmc/articles/PMC3905246/ /pubmed/24445323 http://dx.doi.org/10.1038/ncomms4080 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Munera, Diana
Ritchie, Jennifer M
Hatzios, Stavroula K
Bronson, Rod
Fang, Gang
Schadt, Eric E
Davis, Brigid M
Waldor, Matthew K
Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4
title Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4
title_full Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4
title_fullStr Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4
title_full_unstemmed Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4
title_short Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4
title_sort autotransporters but not paa are critical for rabbit colonization by shiga toxin-producing escherichia coli o104:h4
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905246/
https://www.ncbi.nlm.nih.gov/pubmed/24445323
http://dx.doi.org/10.1038/ncomms4080
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