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Pathogenesis of GIII.2 bovine norovirus, CV186-OH/00/US strain in gnotobiotic calves
The pathogenesis of GIII.2 bovine norovirus (BoNoV) is not well understood. Our study demonstrated persisting diarrhea and prolonged fecal shedding, but with a lack of significant intestinal lesions in gnotobiotic (Gn) calves infected with GIII.2 BoNoV, CV186-OH/00/US strain. Nine 4 to 7-day-old Ang...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Elsevier B.V.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905316/ https://www.ncbi.nlm.nih.gov/pubmed/24309403 http://dx.doi.org/10.1016/j.vetmic.2013.11.008 |
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author | Jung, Kwonil Scheuer, Kelly A. Zhang, Zhenwen Wang, Qiuhong Saif, Linda J. |
author_facet | Jung, Kwonil Scheuer, Kelly A. Zhang, Zhenwen Wang, Qiuhong Saif, Linda J. |
author_sort | Jung, Kwonil |
collection | PubMed |
description | The pathogenesis of GIII.2 bovine norovirus (BoNoV) is not well understood. Our study demonstrated persisting diarrhea and prolonged fecal shedding, but with a lack of significant intestinal lesions in gnotobiotic (Gn) calves infected with GIII.2 BoNoV, CV186-OH/00/US strain. Nine 4 to 7-day-old Angus/Jersey crossbred Gn calves were orally inoculated with 10.0–11.9 log(10) genomic equivalents (GE)/calf of CV186-OH (n = 7) or mock (n = 2). Calves were euthanized at post-inoculation day (PID) 1 (n = 1) when moderate to severe lethargy was observed and at PIDs 2–6 (n = 4) after lethargy had subsided. Two calves were kept longer term (until PID 30) for monitoring fecal shedding patterns by TaqMan real-time RT-PCR (qRT-PCR). Most infected calves exhibited two clinical signs: (i) acute but persisting diarrhea and (ii) acute moderate to severe lethargy. The two infected calves, followed longer-term, had prolonged fecal viral RNA shedding [peak average titer of 11.8 (±0.2) log(10) GE/ml] at least until PID 20. By qRT-PCR, 5 infected calves had low viral RNA titers in serum, ranging from 4.0 to 5.8 log(10) GE/ml, at PIDs 1–5, but not (<2.7 log(10) GE/ml) at PIDs 6–30. The latter observation coincided with the presence of serum IgG antibody to BoNoV at PIDs 8–30. Collectively, the GIII.2 BoNoV strain CV186-OH induced only mild enteropathogenicity, evident by the lack of significant intestinal lesions, but it led to persisting mild diarrhea and prolonged fecal virus shedding in Gn calves. The prolonged fecal shedding of GIII.2 BoNoV might partially explain how this virus is maintained as endemic infections in cattle. |
format | Online Article Text |
id | pubmed-3905316 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-39053162015-01-10 Pathogenesis of GIII.2 bovine norovirus, CV186-OH/00/US strain in gnotobiotic calves Jung, Kwonil Scheuer, Kelly A. Zhang, Zhenwen Wang, Qiuhong Saif, Linda J. Vet Microbiol Short Communication The pathogenesis of GIII.2 bovine norovirus (BoNoV) is not well understood. Our study demonstrated persisting diarrhea and prolonged fecal shedding, but with a lack of significant intestinal lesions in gnotobiotic (Gn) calves infected with GIII.2 BoNoV, CV186-OH/00/US strain. Nine 4 to 7-day-old Angus/Jersey crossbred Gn calves were orally inoculated with 10.0–11.9 log(10) genomic equivalents (GE)/calf of CV186-OH (n = 7) or mock (n = 2). Calves were euthanized at post-inoculation day (PID) 1 (n = 1) when moderate to severe lethargy was observed and at PIDs 2–6 (n = 4) after lethargy had subsided. Two calves were kept longer term (until PID 30) for monitoring fecal shedding patterns by TaqMan real-time RT-PCR (qRT-PCR). Most infected calves exhibited two clinical signs: (i) acute but persisting diarrhea and (ii) acute moderate to severe lethargy. The two infected calves, followed longer-term, had prolonged fecal viral RNA shedding [peak average titer of 11.8 (±0.2) log(10) GE/ml] at least until PID 20. By qRT-PCR, 5 infected calves had low viral RNA titers in serum, ranging from 4.0 to 5.8 log(10) GE/ml, at PIDs 1–5, but not (<2.7 log(10) GE/ml) at PIDs 6–30. The latter observation coincided with the presence of serum IgG antibody to BoNoV at PIDs 8–30. Collectively, the GIII.2 BoNoV strain CV186-OH induced only mild enteropathogenicity, evident by the lack of significant intestinal lesions, but it led to persisting mild diarrhea and prolonged fecal virus shedding in Gn calves. The prolonged fecal shedding of GIII.2 BoNoV might partially explain how this virus is maintained as endemic infections in cattle. Elsevier B.V. 2014-01-10 2013-11-16 /pmc/articles/PMC3905316/ /pubmed/24309403 http://dx.doi.org/10.1016/j.vetmic.2013.11.008 Text en Copyright © 2013 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Short Communication Jung, Kwonil Scheuer, Kelly A. Zhang, Zhenwen Wang, Qiuhong Saif, Linda J. Pathogenesis of GIII.2 bovine norovirus, CV186-OH/00/US strain in gnotobiotic calves |
title | Pathogenesis of GIII.2 bovine norovirus, CV186-OH/00/US strain in gnotobiotic calves |
title_full | Pathogenesis of GIII.2 bovine norovirus, CV186-OH/00/US strain in gnotobiotic calves |
title_fullStr | Pathogenesis of GIII.2 bovine norovirus, CV186-OH/00/US strain in gnotobiotic calves |
title_full_unstemmed | Pathogenesis of GIII.2 bovine norovirus, CV186-OH/00/US strain in gnotobiotic calves |
title_short | Pathogenesis of GIII.2 bovine norovirus, CV186-OH/00/US strain in gnotobiotic calves |
title_sort | pathogenesis of giii.2 bovine norovirus, cv186-oh/00/us strain in gnotobiotic calves |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905316/ https://www.ncbi.nlm.nih.gov/pubmed/24309403 http://dx.doi.org/10.1016/j.vetmic.2013.11.008 |
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