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Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay

Engineered metabolic pathways often suffer from flux imbalances that can overburden the cell and accumulate intermediate metabolites, resulting in reduced product titers. One way to alleviate such imbalances is to adjust the expression levels of the constituent enzymes using a combinatorial expressi...

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Detalles Bibliográficos
Autores principales: Lee, Michael E., Aswani, Anil, Han, Audrey S., Tomlin, Claire J., Dueber, John E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905865/
https://www.ncbi.nlm.nih.gov/pubmed/24038353
http://dx.doi.org/10.1093/nar/gkt809