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Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay
Engineered metabolic pathways often suffer from flux imbalances that can overburden the cell and accumulate intermediate metabolites, resulting in reduced product titers. One way to alleviate such imbalances is to adjust the expression levels of the constituent enzymes using a combinatorial expressi...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3905865/ https://www.ncbi.nlm.nih.gov/pubmed/24038353 http://dx.doi.org/10.1093/nar/gkt809 |