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Novel Quantitative Autophagy Analysis by Organelle Flow Cytometry after Cell Sonication
Autophagy is a dynamic process of bulk degradation of cellular proteins and organelles in lysosomes. Current methods of autophagy measurement include microscopy-based counting of autophagic vacuoles (AVs) in cells. We have developed a novel method to quantitatively analyze individual AVs using flow...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3906200/ https://www.ncbi.nlm.nih.gov/pubmed/24489953 http://dx.doi.org/10.1371/journal.pone.0087707 |