Deep mutational scanning of an antibody against epidermal growth factor receptor using mammalian cell display and massively parallel pyrosequencing

We developed a method for deep mutational scanning of antibody complementarity-determining regions (CDRs) that can determine in parallel the effect of every possible single amino acid CDR substitution on antigen binding. The method uses libraries of full length IgGs containing more than 1000 CDR poi...

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Detalles Bibliográficos
Autores principales: Forsyth, Charles M., Juan, Veronica, Akamatsu, Yoshiko, DuBridge, Robert B., Doan, Minhtam, Ivanov, Alexander V., Ma, Zhiyuan, Polakoff, Dixie, Razo, Jennifer, Wilson, Keith, Powers, David B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3906306/
https://www.ncbi.nlm.nih.gov/pubmed/23765106
http://dx.doi.org/10.4161/mabs.24979

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