Cargando…

Large oncosomes mediate intercellular transfer of functional microRNA

Prostate cancer cells release atypically large extracellular vesicles (EVs), termed large oncosomes, which may play a role in the tumor microenvironment by transporting bioactive molecules across tissue spaces and through the blood stream. In this study, we applied a novel method for selective isola...

Descripción completa

Detalles Bibliográficos
Autores principales: Morello, Matteo, Minciacchi, Valentina R, de Candia, Paola, Yang, Julie, Posadas, Edwin, Kim, Hyung, Griffiths, Duncan, Bhowmick, Neil, Chung, Leland WK, Gandellini, Paolo, Freeman, Michael R, Demichelis, Francesca, Di Vizio, Dolores
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3906338/
https://www.ncbi.nlm.nih.gov/pubmed/24091630
http://dx.doi.org/10.4161/cc.26539
_version_ 1782301474359869440
author Morello, Matteo
Minciacchi, Valentina R
de Candia, Paola
Yang, Julie
Posadas, Edwin
Kim, Hyung
Griffiths, Duncan
Bhowmick, Neil
Chung, Leland WK
Gandellini, Paolo
Freeman, Michael R
Demichelis, Francesca
Di Vizio, Dolores
author_facet Morello, Matteo
Minciacchi, Valentina R
de Candia, Paola
Yang, Julie
Posadas, Edwin
Kim, Hyung
Griffiths, Duncan
Bhowmick, Neil
Chung, Leland WK
Gandellini, Paolo
Freeman, Michael R
Demichelis, Francesca
Di Vizio, Dolores
author_sort Morello, Matteo
collection PubMed
description Prostate cancer cells release atypically large extracellular vesicles (EVs), termed large oncosomes, which may play a role in the tumor microenvironment by transporting bioactive molecules across tissue spaces and through the blood stream. In this study, we applied a novel method for selective isolation of large oncosomes applicable to human platelet-poor plasma, where the presence of caveolin-1-positive large oncosomes identified patients with metastatic disease. This procedure was also used to validate results of a miRNA array performed on heterogeneous populations of EVs isolated from tumorigenic RWPE-2 prostate cells and from isogenic non-tumorigenic RWPE-1 cells. The results showed that distinct classes of miRNAs are expressed at higher levels in EVs derived from the tumorigenic cells in comparison to their non-tumorigenic counterpart. Large oncosomes enhanced migration of cancer-associated fibroblasts (CAFs), an effect that was increased by miR-1227, a miRNA abundant in large oncosomes produced by RWPE-2 cells. Our findings suggest that large oncosomes in the circulation report metastatic disease in patients with prostate cancer, and that this class of EV harbors functional molecules that may play a role in conditioning the tumor microenvironment.
format Online
Article
Text
id pubmed-3906338
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Landes Bioscience
record_format MEDLINE/PubMed
spelling pubmed-39063382014-02-04 Large oncosomes mediate intercellular transfer of functional microRNA Morello, Matteo Minciacchi, Valentina R de Candia, Paola Yang, Julie Posadas, Edwin Kim, Hyung Griffiths, Duncan Bhowmick, Neil Chung, Leland WK Gandellini, Paolo Freeman, Michael R Demichelis, Francesca Di Vizio, Dolores Cell Cycle Report Prostate cancer cells release atypically large extracellular vesicles (EVs), termed large oncosomes, which may play a role in the tumor microenvironment by transporting bioactive molecules across tissue spaces and through the blood stream. In this study, we applied a novel method for selective isolation of large oncosomes applicable to human platelet-poor plasma, where the presence of caveolin-1-positive large oncosomes identified patients with metastatic disease. This procedure was also used to validate results of a miRNA array performed on heterogeneous populations of EVs isolated from tumorigenic RWPE-2 prostate cells and from isogenic non-tumorigenic RWPE-1 cells. The results showed that distinct classes of miRNAs are expressed at higher levels in EVs derived from the tumorigenic cells in comparison to their non-tumorigenic counterpart. Large oncosomes enhanced migration of cancer-associated fibroblasts (CAFs), an effect that was increased by miR-1227, a miRNA abundant in large oncosomes produced by RWPE-2 cells. Our findings suggest that large oncosomes in the circulation report metastatic disease in patients with prostate cancer, and that this class of EV harbors functional molecules that may play a role in conditioning the tumor microenvironment. Landes Bioscience 2013-11-15 2013-09-23 /pmc/articles/PMC3906338/ /pubmed/24091630 http://dx.doi.org/10.4161/cc.26539 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Report
Morello, Matteo
Minciacchi, Valentina R
de Candia, Paola
Yang, Julie
Posadas, Edwin
Kim, Hyung
Griffiths, Duncan
Bhowmick, Neil
Chung, Leland WK
Gandellini, Paolo
Freeman, Michael R
Demichelis, Francesca
Di Vizio, Dolores
Large oncosomes mediate intercellular transfer of functional microRNA
title Large oncosomes mediate intercellular transfer of functional microRNA
title_full Large oncosomes mediate intercellular transfer of functional microRNA
title_fullStr Large oncosomes mediate intercellular transfer of functional microRNA
title_full_unstemmed Large oncosomes mediate intercellular transfer of functional microRNA
title_short Large oncosomes mediate intercellular transfer of functional microRNA
title_sort large oncosomes mediate intercellular transfer of functional microrna
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3906338/
https://www.ncbi.nlm.nih.gov/pubmed/24091630
http://dx.doi.org/10.4161/cc.26539
work_keys_str_mv AT morellomatteo largeoncosomesmediateintercellulartransferoffunctionalmicrorna
AT minciacchivalentinar largeoncosomesmediateintercellulartransferoffunctionalmicrorna
AT decandiapaola largeoncosomesmediateintercellulartransferoffunctionalmicrorna
AT yangjulie largeoncosomesmediateintercellulartransferoffunctionalmicrorna
AT posadasedwin largeoncosomesmediateintercellulartransferoffunctionalmicrorna
AT kimhyung largeoncosomesmediateintercellulartransferoffunctionalmicrorna
AT griffithsduncan largeoncosomesmediateintercellulartransferoffunctionalmicrorna
AT bhowmickneil largeoncosomesmediateintercellulartransferoffunctionalmicrorna
AT chunglelandwk largeoncosomesmediateintercellulartransferoffunctionalmicrorna
AT gandellinipaolo largeoncosomesmediateintercellulartransferoffunctionalmicrorna
AT freemanmichaelr largeoncosomesmediateintercellulartransferoffunctionalmicrorna
AT demichelisfrancesca largeoncosomesmediateintercellulartransferoffunctionalmicrorna
AT diviziodolores largeoncosomesmediateintercellulartransferoffunctionalmicrorna