Cargando…
Large oncosomes mediate intercellular transfer of functional microRNA
Prostate cancer cells release atypically large extracellular vesicles (EVs), termed large oncosomes, which may play a role in the tumor microenvironment by transporting bioactive molecules across tissue spaces and through the blood stream. In this study, we applied a novel method for selective isola...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Landes Bioscience
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3906338/ https://www.ncbi.nlm.nih.gov/pubmed/24091630 http://dx.doi.org/10.4161/cc.26539 |
_version_ | 1782301474359869440 |
---|---|
author | Morello, Matteo Minciacchi, Valentina R de Candia, Paola Yang, Julie Posadas, Edwin Kim, Hyung Griffiths, Duncan Bhowmick, Neil Chung, Leland WK Gandellini, Paolo Freeman, Michael R Demichelis, Francesca Di Vizio, Dolores |
author_facet | Morello, Matteo Minciacchi, Valentina R de Candia, Paola Yang, Julie Posadas, Edwin Kim, Hyung Griffiths, Duncan Bhowmick, Neil Chung, Leland WK Gandellini, Paolo Freeman, Michael R Demichelis, Francesca Di Vizio, Dolores |
author_sort | Morello, Matteo |
collection | PubMed |
description | Prostate cancer cells release atypically large extracellular vesicles (EVs), termed large oncosomes, which may play a role in the tumor microenvironment by transporting bioactive molecules across tissue spaces and through the blood stream. In this study, we applied a novel method for selective isolation of large oncosomes applicable to human platelet-poor plasma, where the presence of caveolin-1-positive large oncosomes identified patients with metastatic disease. This procedure was also used to validate results of a miRNA array performed on heterogeneous populations of EVs isolated from tumorigenic RWPE-2 prostate cells and from isogenic non-tumorigenic RWPE-1 cells. The results showed that distinct classes of miRNAs are expressed at higher levels in EVs derived from the tumorigenic cells in comparison to their non-tumorigenic counterpart. Large oncosomes enhanced migration of cancer-associated fibroblasts (CAFs), an effect that was increased by miR-1227, a miRNA abundant in large oncosomes produced by RWPE-2 cells. Our findings suggest that large oncosomes in the circulation report metastatic disease in patients with prostate cancer, and that this class of EV harbors functional molecules that may play a role in conditioning the tumor microenvironment. |
format | Online Article Text |
id | pubmed-3906338 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Landes Bioscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-39063382014-02-04 Large oncosomes mediate intercellular transfer of functional microRNA Morello, Matteo Minciacchi, Valentina R de Candia, Paola Yang, Julie Posadas, Edwin Kim, Hyung Griffiths, Duncan Bhowmick, Neil Chung, Leland WK Gandellini, Paolo Freeman, Michael R Demichelis, Francesca Di Vizio, Dolores Cell Cycle Report Prostate cancer cells release atypically large extracellular vesicles (EVs), termed large oncosomes, which may play a role in the tumor microenvironment by transporting bioactive molecules across tissue spaces and through the blood stream. In this study, we applied a novel method for selective isolation of large oncosomes applicable to human platelet-poor plasma, where the presence of caveolin-1-positive large oncosomes identified patients with metastatic disease. This procedure was also used to validate results of a miRNA array performed on heterogeneous populations of EVs isolated from tumorigenic RWPE-2 prostate cells and from isogenic non-tumorigenic RWPE-1 cells. The results showed that distinct classes of miRNAs are expressed at higher levels in EVs derived from the tumorigenic cells in comparison to their non-tumorigenic counterpart. Large oncosomes enhanced migration of cancer-associated fibroblasts (CAFs), an effect that was increased by miR-1227, a miRNA abundant in large oncosomes produced by RWPE-2 cells. Our findings suggest that large oncosomes in the circulation report metastatic disease in patients with prostate cancer, and that this class of EV harbors functional molecules that may play a role in conditioning the tumor microenvironment. Landes Bioscience 2013-11-15 2013-09-23 /pmc/articles/PMC3906338/ /pubmed/24091630 http://dx.doi.org/10.4161/cc.26539 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Report Morello, Matteo Minciacchi, Valentina R de Candia, Paola Yang, Julie Posadas, Edwin Kim, Hyung Griffiths, Duncan Bhowmick, Neil Chung, Leland WK Gandellini, Paolo Freeman, Michael R Demichelis, Francesca Di Vizio, Dolores Large oncosomes mediate intercellular transfer of functional microRNA |
title | Large oncosomes mediate intercellular transfer of functional microRNA |
title_full | Large oncosomes mediate intercellular transfer of functional microRNA |
title_fullStr | Large oncosomes mediate intercellular transfer of functional microRNA |
title_full_unstemmed | Large oncosomes mediate intercellular transfer of functional microRNA |
title_short | Large oncosomes mediate intercellular transfer of functional microRNA |
title_sort | large oncosomes mediate intercellular transfer of functional microrna |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3906338/ https://www.ncbi.nlm.nih.gov/pubmed/24091630 http://dx.doi.org/10.4161/cc.26539 |
work_keys_str_mv | AT morellomatteo largeoncosomesmediateintercellulartransferoffunctionalmicrorna AT minciacchivalentinar largeoncosomesmediateintercellulartransferoffunctionalmicrorna AT decandiapaola largeoncosomesmediateintercellulartransferoffunctionalmicrorna AT yangjulie largeoncosomesmediateintercellulartransferoffunctionalmicrorna AT posadasedwin largeoncosomesmediateintercellulartransferoffunctionalmicrorna AT kimhyung largeoncosomesmediateintercellulartransferoffunctionalmicrorna AT griffithsduncan largeoncosomesmediateintercellulartransferoffunctionalmicrorna AT bhowmickneil largeoncosomesmediateintercellulartransferoffunctionalmicrorna AT chunglelandwk largeoncosomesmediateintercellulartransferoffunctionalmicrorna AT gandellinipaolo largeoncosomesmediateintercellulartransferoffunctionalmicrorna AT freemanmichaelr largeoncosomesmediateintercellulartransferoffunctionalmicrorna AT demichelisfrancesca largeoncosomesmediateintercellulartransferoffunctionalmicrorna AT diviziodolores largeoncosomesmediateintercellulartransferoffunctionalmicrorna |