A microarray method for identifying tumor antigens by screening a tumor cDNA expression library against cancer sera

The immune system responds to tumor cells. The challenge has been how to effectively use these responses to treat or protect against cancer. Toward the goal of developing a cancer vaccine, we are pursuing methodologies for the discovery and testing of useful antigens. We present an array-based appro...

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Detalles Bibliográficos
Autores principales: Whittemore, Kurt, Sykes, Kathryn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3906402/
https://www.ncbi.nlm.nih.gov/pubmed/23851590
http://dx.doi.org/10.4161/hv.25634
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author Whittemore, Kurt
Sykes, Kathryn
author_facet Whittemore, Kurt
Sykes, Kathryn
author_sort Whittemore, Kurt
collection PubMed
description The immune system responds to tumor cells. The challenge has been how to effectively use these responses to treat or protect against cancer. Toward the goal of developing a cancer vaccine, we are pursuing methodologies for the discovery and testing of useful antigens. We present an array-based approach for discovering these B cell antigens by directly screening for specific host-sera reactivity to lysates from tumor-derived cDNA expression libraries. Several cancer-specific antigens were identified, and these are currently being validated as potential candidates.
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spelling pubmed-39064022014-02-07 A microarray method for identifying tumor antigens by screening a tumor cDNA expression library against cancer sera Whittemore, Kurt Sykes, Kathryn Hum Vaccin Immunother Research Paper The immune system responds to tumor cells. The challenge has been how to effectively use these responses to treat or protect against cancer. Toward the goal of developing a cancer vaccine, we are pursuing methodologies for the discovery and testing of useful antigens. We present an array-based approach for discovering these B cell antigens by directly screening for specific host-sera reactivity to lysates from tumor-derived cDNA expression libraries. Several cancer-specific antigens were identified, and these are currently being validated as potential candidates. Landes Bioscience 2013-10-01 2013-07-12 /pmc/articles/PMC3906402/ /pubmed/23851590 http://dx.doi.org/10.4161/hv.25634 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Research Paper
Whittemore, Kurt
Sykes, Kathryn
A microarray method for identifying tumor antigens by screening a tumor cDNA expression library against cancer sera
title A microarray method for identifying tumor antigens by screening a tumor cDNA expression library against cancer sera
title_full A microarray method for identifying tumor antigens by screening a tumor cDNA expression library against cancer sera
title_fullStr A microarray method for identifying tumor antigens by screening a tumor cDNA expression library against cancer sera
title_full_unstemmed A microarray method for identifying tumor antigens by screening a tumor cDNA expression library against cancer sera
title_short A microarray method for identifying tumor antigens by screening a tumor cDNA expression library against cancer sera
title_sort microarray method for identifying tumor antigens by screening a tumor cdna expression library against cancer sera
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3906402/
https://www.ncbi.nlm.nih.gov/pubmed/23851590
http://dx.doi.org/10.4161/hv.25634
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