Conventional transmission electron microscopy

Researchers have used transmission electron microscopy (TEM) to make contributions to cell biology for well over 50 years, and TEM continues to be an important technology in our field. We briefly present for the neophyte the components of a TEM-based study, beginning with sample preparation through...

Descripción completa

Detalles Bibliográficos
Autores principales: Winey, Mark, Meehl, Janet B., O'Toole, Eileen T., Giddings, Thomas H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
MBoC Technical Perspective
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907272/
https://www.ncbi.nlm.nih.gov/pubmed/24482357
http://dx.doi.org/10.1091/mbc.E12-12-0863
Descripción
Sumario:Researchers have used transmission electron microscopy (TEM) to make contributions to cell biology for well over 50 years, and TEM continues to be an important technology in our field. We briefly present for the neophyte the components of a TEM-based study, beginning with sample preparation through imaging of the samples. We point out the limitations of TEM and issues to be considered during experimental design. Advanced electron microscopy techniques are listed as well. Finally, we point potential new users of TEM to resources to help launch their project.

Ejemplares similares