Conventional transmission electron microscopy

Researchers have used transmission electron microscopy (TEM) to make contributions to cell biology for well over 50 years, and TEM continues to be an important technology in our field. We briefly present for the neophyte the components of a TEM-based study, beginning with sample preparation through...

Descripción completa

Detalles Bibliográficos
Autores principales: Winey, Mark, Meehl, Janet B., O'Toole, Eileen T., Giddings, Thomas H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
MBoC Technical Perspective
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907272/
https://www.ncbi.nlm.nih.gov/pubmed/24482357
http://dx.doi.org/10.1091/mbc.E12-12-0863
_version_ 1782301577280749568
author Winey, Mark
Meehl, Janet B.
O'Toole, Eileen T.
Giddings, Thomas H.
author_facet Winey, Mark
Meehl, Janet B.
O'Toole, Eileen T.
Giddings, Thomas H.
author_sort Winey, Mark
collection PubMed
description Researchers have used transmission electron microscopy (TEM) to make contributions to cell biology for well over 50 years, and TEM continues to be an important technology in our field. We briefly present for the neophyte the components of a TEM-based study, beginning with sample preparation through imaging of the samples. We point out the limitations of TEM and issues to be considered during experimental design. Advanced electron microscopy techniques are listed as well. Finally, we point potential new users of TEM to resources to help launch their project.
format Online
Article
Text
id pubmed-3907272
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-39072722014-04-16 Conventional transmission electron microscopy Winey, Mark Meehl, Janet B. O'Toole, Eileen T. Giddings, Thomas H. Mol Biol Cell MBoC Technical Perspective Researchers have used transmission electron microscopy (TEM) to make contributions to cell biology for well over 50 years, and TEM continues to be an important technology in our field. We briefly present for the neophyte the components of a TEM-based study, beginning with sample preparation through imaging of the samples. We point out the limitations of TEM and issues to be considered during experimental design. Advanced electron microscopy techniques are listed as well. Finally, we point potential new users of TEM to resources to help launch their project. The American Society for Cell Biology 2014-02-01 /pmc/articles/PMC3907272/ /pubmed/24482357 http://dx.doi.org/10.1091/mbc.E12-12-0863 Text en © 2014 Winey et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle MBoC Technical Perspective
Winey, Mark
Meehl, Janet B.
O'Toole, Eileen T.
Giddings, Thomas H.
Conventional transmission electron microscopy
title Conventional transmission electron microscopy
title_full Conventional transmission electron microscopy
title_fullStr Conventional transmission electron microscopy
title_full_unstemmed Conventional transmission electron microscopy
title_short Conventional transmission electron microscopy
title_sort conventional transmission electron microscopy
topic MBoC Technical Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907272/
https://www.ncbi.nlm.nih.gov/pubmed/24482357
http://dx.doi.org/10.1091/mbc.E12-12-0863
work_keys_str_mv AT wineymark conventionaltransmissionelectronmicroscopy
AT meehljanetb conventionaltransmissionelectronmicroscopy
AT otooleeileent conventionaltransmissionelectronmicroscopy
AT giddingsthomash conventionaltransmissionelectronmicroscopy

Ejemplares similares