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First Detection of Mycobacterium ulcerans DNA in Environmental Samples from South America
The occurrences of many environmentally-persistent and zoonotic infections are driven by ecosystem changes, which in turn are underpinned by land-use modifications that alter the governance of pathogen, biodiversity and human interactions. Our current understanding of these ecological changes on dis...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907311/ https://www.ncbi.nlm.nih.gov/pubmed/24498449 http://dx.doi.org/10.1371/journal.pntd.0002660 |
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author | Morris, Aaron Gozlan, Rodolphe Marion, Estelle Marsollier, Laurent Andreou, Demetra Sanhueza, Daniel Ruffine, Rolland Couppié, Pierre Guégan, Jean-François |
author_facet | Morris, Aaron Gozlan, Rodolphe Marion, Estelle Marsollier, Laurent Andreou, Demetra Sanhueza, Daniel Ruffine, Rolland Couppié, Pierre Guégan, Jean-François |
author_sort | Morris, Aaron |
collection | PubMed |
description | The occurrences of many environmentally-persistent and zoonotic infections are driven by ecosystem changes, which in turn are underpinned by land-use modifications that alter the governance of pathogen, biodiversity and human interactions. Our current understanding of these ecological changes on disease emergence however remains limited. Buruli ulcer is an emerging human skin disease caused by the mycobacterium, Mycobacterium ulcerans, for which the exact route of infection remains unclear. It can have a devastating impact on its human host, causing extensive necrosis of the skin and underlying tissue, often leading to permanent disability. The mycobacterium is associated with tropical aquatic environments and incidences of the disease are significantly higher on floodplains and where there is an increase of human aquatic activities. Although the disease has been previously diagnosed in South America, until now the presence of M. ulcerans DNA in the wild has only been identified in Australia where there have been significant outbreaks and in western and central regions of Africa where the disease is persistent. Here for the first time, we have identified the presence of the aetiological agent's DNA in environmental samples from South America. The DNA was positively identified using Real-time Polymerase Chain Reaction (PCR) on 163 environmental samples, taken from 23 freshwater bodies in French Guiana (Southern America), using primers for both IS2404 and for the ketoreductase-B domain of the M. ulcerans mycolactone polyketide synthase genes (KR). Five samples out of 163 were positive for both primers from three different water bodies. A further nine sites had low levels of IS2404 close to a standard CT of 35 and could potentially harbour M. ulcerans. The majority of our positive samples (8/14) came from filtered water. These results also reveal the Sinnamary River as a potential source of infection to humans. |
format | Online Article Text |
id | pubmed-3907311 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39073112014-02-04 First Detection of Mycobacterium ulcerans DNA in Environmental Samples from South America Morris, Aaron Gozlan, Rodolphe Marion, Estelle Marsollier, Laurent Andreou, Demetra Sanhueza, Daniel Ruffine, Rolland Couppié, Pierre Guégan, Jean-François PLoS Negl Trop Dis Research Article The occurrences of many environmentally-persistent and zoonotic infections are driven by ecosystem changes, which in turn are underpinned by land-use modifications that alter the governance of pathogen, biodiversity and human interactions. Our current understanding of these ecological changes on disease emergence however remains limited. Buruli ulcer is an emerging human skin disease caused by the mycobacterium, Mycobacterium ulcerans, for which the exact route of infection remains unclear. It can have a devastating impact on its human host, causing extensive necrosis of the skin and underlying tissue, often leading to permanent disability. The mycobacterium is associated with tropical aquatic environments and incidences of the disease are significantly higher on floodplains and where there is an increase of human aquatic activities. Although the disease has been previously diagnosed in South America, until now the presence of M. ulcerans DNA in the wild has only been identified in Australia where there have been significant outbreaks and in western and central regions of Africa where the disease is persistent. Here for the first time, we have identified the presence of the aetiological agent's DNA in environmental samples from South America. The DNA was positively identified using Real-time Polymerase Chain Reaction (PCR) on 163 environmental samples, taken from 23 freshwater bodies in French Guiana (Southern America), using primers for both IS2404 and for the ketoreductase-B domain of the M. ulcerans mycolactone polyketide synthase genes (KR). Five samples out of 163 were positive for both primers from three different water bodies. A further nine sites had low levels of IS2404 close to a standard CT of 35 and could potentially harbour M. ulcerans. The majority of our positive samples (8/14) came from filtered water. These results also reveal the Sinnamary River as a potential source of infection to humans. Public Library of Science 2014-01-30 /pmc/articles/PMC3907311/ /pubmed/24498449 http://dx.doi.org/10.1371/journal.pntd.0002660 Text en © 2014 Morris et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Morris, Aaron Gozlan, Rodolphe Marion, Estelle Marsollier, Laurent Andreou, Demetra Sanhueza, Daniel Ruffine, Rolland Couppié, Pierre Guégan, Jean-François First Detection of Mycobacterium ulcerans DNA in Environmental Samples from South America |
title | First Detection of Mycobacterium ulcerans DNA in Environmental Samples from South America |
title_full | First Detection of Mycobacterium ulcerans DNA in Environmental Samples from South America |
title_fullStr | First Detection of Mycobacterium ulcerans DNA in Environmental Samples from South America |
title_full_unstemmed | First Detection of Mycobacterium ulcerans DNA in Environmental Samples from South America |
title_short | First Detection of Mycobacterium ulcerans DNA in Environmental Samples from South America |
title_sort | first detection of mycobacterium ulcerans dna in environmental samples from south america |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907311/ https://www.ncbi.nlm.nih.gov/pubmed/24498449 http://dx.doi.org/10.1371/journal.pntd.0002660 |
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