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High-Resolution Echo-Planar Spectroscopic Imaging of the Human Calf

BACKGROUND: This study exploits the speed benefits of echo-planar spectroscopic imaging (EPSI) to acquire lipid spectra of skeletal muscle. The main purpose was to develop a high-resolution EPSI technique for clinical MR scanner, to visualise the bulk magnetic susceptibility (BMS) shifts of extra-my...

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Autores principales: Weis, Jan, Bruvold, Morten, Ortiz-Nieto, Francisco, Ahlström, Håkan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907517/
https://www.ncbi.nlm.nih.gov/pubmed/24498129
http://dx.doi.org/10.1371/journal.pone.0087533
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author Weis, Jan
Bruvold, Morten
Ortiz-Nieto, Francisco
Ahlström, Håkan
author_facet Weis, Jan
Bruvold, Morten
Ortiz-Nieto, Francisco
Ahlström, Håkan
author_sort Weis, Jan
collection PubMed
description BACKGROUND: This study exploits the speed benefits of echo-planar spectroscopic imaging (EPSI) to acquire lipid spectra of skeletal muscle. The main purpose was to develop a high-resolution EPSI technique for clinical MR scanner, to visualise the bulk magnetic susceptibility (BMS) shifts of extra-myocellular lipid (EMCL) spectral lines, and to investigate the feasibility of this method for the assessment of intra-myocellular (IMCL) lipids. METHODS: The study group consisted of six healthy volunteers. A two dimensional EPSI sequence with point-resolved spectroscopy (PRESS) spatial localization was implemented on a 3T clinical MR scanner. Measurements were performed by means of 64×64 spatial matrix and nominal voxel size 3×3×15 mm(3). The total net measurement time was 3 min 12 sec for non-water-suppressed (1 acquisition) and 12 min 48 sec for water-suppressed scans (4 acquisitions). RESULTS: Spectra of the human calf had a very good signal-to-noise ratio and linewidths sufficient to differentiate IMCL resonances from EMCL. The use of a large spatial matrix reduces inter-voxel signal contamination of the strong EMCL signals. Small voxels enabled visualisation of the methylene EMCL spectral line splitting and their BMS shifts up to 0.5 ppm relative to the correspondent IMCL line. The mean soleus muscle IMCL content of our six volunteers was 0.30±0.10 vol% (range 0.18–0.46) or 3.6±1.2 mmol/kg wet weight (range: 2.1–5.4). CONCLUSION: This study demonstrates that high-spatial resolution PRESS EPSI of the muscle lipids is feasible on standard clinical scanners.
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spelling pubmed-39075172014-02-04 High-Resolution Echo-Planar Spectroscopic Imaging of the Human Calf Weis, Jan Bruvold, Morten Ortiz-Nieto, Francisco Ahlström, Håkan PLoS One Research Article BACKGROUND: This study exploits the speed benefits of echo-planar spectroscopic imaging (EPSI) to acquire lipid spectra of skeletal muscle. The main purpose was to develop a high-resolution EPSI technique for clinical MR scanner, to visualise the bulk magnetic susceptibility (BMS) shifts of extra-myocellular lipid (EMCL) spectral lines, and to investigate the feasibility of this method for the assessment of intra-myocellular (IMCL) lipids. METHODS: The study group consisted of six healthy volunteers. A two dimensional EPSI sequence with point-resolved spectroscopy (PRESS) spatial localization was implemented on a 3T clinical MR scanner. Measurements were performed by means of 64×64 spatial matrix and nominal voxel size 3×3×15 mm(3). The total net measurement time was 3 min 12 sec for non-water-suppressed (1 acquisition) and 12 min 48 sec for water-suppressed scans (4 acquisitions). RESULTS: Spectra of the human calf had a very good signal-to-noise ratio and linewidths sufficient to differentiate IMCL resonances from EMCL. The use of a large spatial matrix reduces inter-voxel signal contamination of the strong EMCL signals. Small voxels enabled visualisation of the methylene EMCL spectral line splitting and their BMS shifts up to 0.5 ppm relative to the correspondent IMCL line. The mean soleus muscle IMCL content of our six volunteers was 0.30±0.10 vol% (range 0.18–0.46) or 3.6±1.2 mmol/kg wet weight (range: 2.1–5.4). CONCLUSION: This study demonstrates that high-spatial resolution PRESS EPSI of the muscle lipids is feasible on standard clinical scanners. Public Library of Science 2014-01-30 /pmc/articles/PMC3907517/ /pubmed/24498129 http://dx.doi.org/10.1371/journal.pone.0087533 Text en © 2014 Weis et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Weis, Jan
Bruvold, Morten
Ortiz-Nieto, Francisco
Ahlström, Håkan
High-Resolution Echo-Planar Spectroscopic Imaging of the Human Calf
title High-Resolution Echo-Planar Spectroscopic Imaging of the Human Calf
title_full High-Resolution Echo-Planar Spectroscopic Imaging of the Human Calf
title_fullStr High-Resolution Echo-Planar Spectroscopic Imaging of the Human Calf
title_full_unstemmed High-Resolution Echo-Planar Spectroscopic Imaging of the Human Calf
title_short High-Resolution Echo-Planar Spectroscopic Imaging of the Human Calf
title_sort high-resolution echo-planar spectroscopic imaging of the human calf
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907517/
https://www.ncbi.nlm.nih.gov/pubmed/24498129
http://dx.doi.org/10.1371/journal.pone.0087533
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