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An improved medium for the anaerobic growth of Paracoccus denitrificans Pd1222

Paracoccus denitrificans is a well studied model organism with respect to its aerobic and anaerobic respiratory enzymes. However, until now, the growth medium for this organism has not been optimized for anaerobic growth. In particular, the requirements of P. denitrificans for trace elements (TEs) a...

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Autores principales: Hahnke, Stefanie M., Moosmann, Philipp, Erb, Tobias J., Strous, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907716/
https://www.ncbi.nlm.nih.gov/pubmed/24550891
http://dx.doi.org/10.3389/fmicb.2014.00018
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author Hahnke, Stefanie M.
Moosmann, Philipp
Erb, Tobias J.
Strous, Marc
author_facet Hahnke, Stefanie M.
Moosmann, Philipp
Erb, Tobias J.
Strous, Marc
author_sort Hahnke, Stefanie M.
collection PubMed
description Paracoccus denitrificans is a well studied model organism with respect to its aerobic and anaerobic respiratory enzymes. However, until now, the growth medium for this organism has not been optimized for anaerobic growth. In particular, the requirements of P. denitrificans for trace elements (TEs) are not well known. In the present study we aimed to improve growth rates of P. denitrificans Pd1222 on a defined medium under anoxic conditions. We designed media containing different combinations of TEs at various concentrations, and tested their performance against previously reported media. Our results suggest that growth rate and yield depend on the availability and concentration of TEs in the medium. A chelated TE solution was more suitable than an acidified TE solution. Highest growth rates were achieved with medium comprising the TEs iron, manganese, molybdenum, copper and zinc ranging from 0.1 to 9 μM. On this medium, P. denitrificans Pd1222 grew with a generation time of 4.4 h under anoxic conditions and 2.8 h under oxic conditions. Diauxic growth was clearly shown with respect to nitrate and nitrite reduction under anoxic conditions.
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spelling pubmed-39077162014-02-18 An improved medium for the anaerobic growth of Paracoccus denitrificans Pd1222 Hahnke, Stefanie M. Moosmann, Philipp Erb, Tobias J. Strous, Marc Front Microbiol Microbiology Paracoccus denitrificans is a well studied model organism with respect to its aerobic and anaerobic respiratory enzymes. However, until now, the growth medium for this organism has not been optimized for anaerobic growth. In particular, the requirements of P. denitrificans for trace elements (TEs) are not well known. In the present study we aimed to improve growth rates of P. denitrificans Pd1222 on a defined medium under anoxic conditions. We designed media containing different combinations of TEs at various concentrations, and tested their performance against previously reported media. Our results suggest that growth rate and yield depend on the availability and concentration of TEs in the medium. A chelated TE solution was more suitable than an acidified TE solution. Highest growth rates were achieved with medium comprising the TEs iron, manganese, molybdenum, copper and zinc ranging from 0.1 to 9 μM. On this medium, P. denitrificans Pd1222 grew with a generation time of 4.4 h under anoxic conditions and 2.8 h under oxic conditions. Diauxic growth was clearly shown with respect to nitrate and nitrite reduction under anoxic conditions. Frontiers Media S.A. 2014-01-31 /pmc/articles/PMC3907716/ /pubmed/24550891 http://dx.doi.org/10.3389/fmicb.2014.00018 Text en Copyright © 2014 Hahnke, Moosmann, Erb and Strous. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hahnke, Stefanie M.
Moosmann, Philipp
Erb, Tobias J.
Strous, Marc
An improved medium for the anaerobic growth of Paracoccus denitrificans Pd1222
title An improved medium for the anaerobic growth of Paracoccus denitrificans Pd1222
title_full An improved medium for the anaerobic growth of Paracoccus denitrificans Pd1222
title_fullStr An improved medium for the anaerobic growth of Paracoccus denitrificans Pd1222
title_full_unstemmed An improved medium for the anaerobic growth of Paracoccus denitrificans Pd1222
title_short An improved medium for the anaerobic growth of Paracoccus denitrificans Pd1222
title_sort improved medium for the anaerobic growth of paracoccus denitrificans pd1222
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907716/
https://www.ncbi.nlm.nih.gov/pubmed/24550891
http://dx.doi.org/10.3389/fmicb.2014.00018
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