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Circulating MicroRNAs as Biomarkers of Acute Stroke
MicroRNAs have been identified as key regulators of gene expression and thus their potential in disease diagnostics, prognosis and therapy is being actively pursued. Deregulation of microRNAs in cerebral pathogenesis has been reported to a limited extent in both animal models and human. Due to the c...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907877/ https://www.ncbi.nlm.nih.gov/pubmed/24447930 http://dx.doi.org/10.3390/ijms15011418 |
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author | Sepramaniam, Sugunavathi Tan, Jun-Rong Tan, Kay-Sin DeSilva, Deidre Ann Tavintharan, Subramaniam Woon, Fung-Peng Wang, Chee-Woon Yong, Fung-Lin Karolina, Dwi-Setyowati Kaur, Prameet Liu, Fu-Jia Lim, Kai-Ying Armugam, Arunmozhiarasi Jeyaseelan, Kandiah |
author_facet | Sepramaniam, Sugunavathi Tan, Jun-Rong Tan, Kay-Sin DeSilva, Deidre Ann Tavintharan, Subramaniam Woon, Fung-Peng Wang, Chee-Woon Yong, Fung-Lin Karolina, Dwi-Setyowati Kaur, Prameet Liu, Fu-Jia Lim, Kai-Ying Armugam, Arunmozhiarasi Jeyaseelan, Kandiah |
author_sort | Sepramaniam, Sugunavathi |
collection | PubMed |
description | MicroRNAs have been identified as key regulators of gene expression and thus their potential in disease diagnostics, prognosis and therapy is being actively pursued. Deregulation of microRNAs in cerebral pathogenesis has been reported to a limited extent in both animal models and human. Due to the complexity of the pathology, identifying stroke specific microRNAs has been a challenge. This study shows that microRNA profiles reflect not only the temporal progression of stroke but also the specific etiologies. A panel of 32 microRNAs, which could differentiate stroke etiologies during acute phase was identified and verified using a customized TaqMan Low Density Array (TLDA). Furthermore we also found 5 microRNAs, miR-125b-2*, -27a*, -422a, -488 and -627 to be consistently altered in acute stroke irrespective of age or severity or confounding metabolic complications. Differential expression of these 5 microRNAs was also observed in rat stroke models. Hence, their specificity to the stroke pathology emphasizes the possibility of developing these microRNAs into accurate and useful tools for diagnosis of stroke. |
format | Online Article Text |
id | pubmed-3907877 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-39078772014-01-31 Circulating MicroRNAs as Biomarkers of Acute Stroke Sepramaniam, Sugunavathi Tan, Jun-Rong Tan, Kay-Sin DeSilva, Deidre Ann Tavintharan, Subramaniam Woon, Fung-Peng Wang, Chee-Woon Yong, Fung-Lin Karolina, Dwi-Setyowati Kaur, Prameet Liu, Fu-Jia Lim, Kai-Ying Armugam, Arunmozhiarasi Jeyaseelan, Kandiah Int J Mol Sci Article MicroRNAs have been identified as key regulators of gene expression and thus their potential in disease diagnostics, prognosis and therapy is being actively pursued. Deregulation of microRNAs in cerebral pathogenesis has been reported to a limited extent in both animal models and human. Due to the complexity of the pathology, identifying stroke specific microRNAs has been a challenge. This study shows that microRNA profiles reflect not only the temporal progression of stroke but also the specific etiologies. A panel of 32 microRNAs, which could differentiate stroke etiologies during acute phase was identified and verified using a customized TaqMan Low Density Array (TLDA). Furthermore we also found 5 microRNAs, miR-125b-2*, -27a*, -422a, -488 and -627 to be consistently altered in acute stroke irrespective of age or severity or confounding metabolic complications. Differential expression of these 5 microRNAs was also observed in rat stroke models. Hence, their specificity to the stroke pathology emphasizes the possibility of developing these microRNAs into accurate and useful tools for diagnosis of stroke. Molecular Diversity Preservation International (MDPI) 2014-01-20 /pmc/articles/PMC3907877/ /pubmed/24447930 http://dx.doi.org/10.3390/ijms15011418 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Sepramaniam, Sugunavathi Tan, Jun-Rong Tan, Kay-Sin DeSilva, Deidre Ann Tavintharan, Subramaniam Woon, Fung-Peng Wang, Chee-Woon Yong, Fung-Lin Karolina, Dwi-Setyowati Kaur, Prameet Liu, Fu-Jia Lim, Kai-Ying Armugam, Arunmozhiarasi Jeyaseelan, Kandiah Circulating MicroRNAs as Biomarkers of Acute Stroke |
title | Circulating MicroRNAs as Biomarkers of Acute Stroke |
title_full | Circulating MicroRNAs as Biomarkers of Acute Stroke |
title_fullStr | Circulating MicroRNAs as Biomarkers of Acute Stroke |
title_full_unstemmed | Circulating MicroRNAs as Biomarkers of Acute Stroke |
title_short | Circulating MicroRNAs as Biomarkers of Acute Stroke |
title_sort | circulating micrornas as biomarkers of acute stroke |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907877/ https://www.ncbi.nlm.nih.gov/pubmed/24447930 http://dx.doi.org/10.3390/ijms15011418 |
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