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The Frequency of Proliferative Stromal Cells in Adipose Tissue Varies Between Inbred Mouse Strains
Stromal cells derived from adipose tissue (ASCs) can proliferate as undifferentiated cells with a fibroblast-like morphology in cell culture, or can be induced to differentiate into a variety of cell types including, adipipogenic, myogenic, neurogenic, osteogenic, chondrogenic and hepatic cells. The...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Journal of Stem cells and Regenerative medicine
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3908149/ https://www.ncbi.nlm.nih.gov/pubmed/24693038 |
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author | Mo, J Srour, EF Rosen, ED |
author_facet | Mo, J Srour, EF Rosen, ED |
author_sort | Mo, J |
collection | PubMed |
description | Stromal cells derived from adipose tissue (ASCs) can proliferate as undifferentiated cells with a fibroblast-like morphology in cell culture, or can be induced to differentiate into a variety of cell types including, adipipogenic, myogenic, neurogenic, osteogenic, chondrogenic and hepatic cells. There is increasing interest to understand the factors controlling the proliferation of ASCs since these cells might provide a readily available source of autologous stem/progenitor cells for cell therapy applications. To explore potential genetic factors that modify the properties of ASCs, we tried to identify relevant properties of ASCs that differ between inbred mouse strains. Plating cells in a modified colony forming assay indicates that the percentage of high proliferative cells among ASCs differs more than 2-fold between 129x1/svj and C57Bl/6J mice. The identification of genetic factors affecting the proliferative capacity of stem cell populations could improve the efficacy of cell therapy. |
format | Online Article Text |
id | pubmed-3908149 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Journal of Stem cells and Regenerative medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-39081492014-04-01 The Frequency of Proliferative Stromal Cells in Adipose Tissue Varies Between Inbred Mouse Strains Mo, J Srour, EF Rosen, ED J Stem Cells Regen Med Original Article Stromal cells derived from adipose tissue (ASCs) can proliferate as undifferentiated cells with a fibroblast-like morphology in cell culture, or can be induced to differentiate into a variety of cell types including, adipipogenic, myogenic, neurogenic, osteogenic, chondrogenic and hepatic cells. There is increasing interest to understand the factors controlling the proliferation of ASCs since these cells might provide a readily available source of autologous stem/progenitor cells for cell therapy applications. To explore potential genetic factors that modify the properties of ASCs, we tried to identify relevant properties of ASCs that differ between inbred mouse strains. Plating cells in a modified colony forming assay indicates that the percentage of high proliferative cells among ASCs differs more than 2-fold between 129x1/svj and C57Bl/6J mice. The identification of genetic factors affecting the proliferative capacity of stem cell populations could improve the efficacy of cell therapy. Journal of Stem cells and Regenerative medicine 2009-04-08 /pmc/articles/PMC3908149/ /pubmed/24693038 Text en Copyright © 2009 Journal of Stem cells and Regenerative medicine http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Mo, J Srour, EF Rosen, ED The Frequency of Proliferative Stromal Cells in Adipose Tissue Varies Between Inbred Mouse Strains |
title | The Frequency of Proliferative Stromal Cells in Adipose Tissue Varies Between Inbred Mouse Strains |
title_full | The Frequency of Proliferative Stromal Cells in Adipose Tissue Varies Between Inbred Mouse Strains |
title_fullStr | The Frequency of Proliferative Stromal Cells in Adipose Tissue Varies Between Inbred Mouse Strains |
title_full_unstemmed | The Frequency of Proliferative Stromal Cells in Adipose Tissue Varies Between Inbred Mouse Strains |
title_short | The Frequency of Proliferative Stromal Cells in Adipose Tissue Varies Between Inbred Mouse Strains |
title_sort | frequency of proliferative stromal cells in adipose tissue varies between inbred mouse strains |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3908149/ https://www.ncbi.nlm.nih.gov/pubmed/24693038 |
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