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Nur77 Decreases Atherosclerosis Progression in apoE(−/−) Mice Fed a High-Fat/High-Cholesterol Diet

RATIONALE: It is clear that lipid disorder and inflammation are associated with cardiovascular diseases and underlying atherosclerosis. Nur77 has been shown to be involved in inflammatory response and lipid metabolism. OBJECTIVE: Here, we explored the role of Nur77 in atherosclerotic plaque progress...

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Autores principales: Hu, Yan-Wei, Zhang, Peng, Yang, Jun-Yao, Huang, Jin-Lan, Ma, Xin, Li, Shu-Fen, Zhao, Jia-Yi, Hu, Ya-Rong, Wang, Yan-Chao, Gao, Ji-Juan, Sha, Yan-Hua, Zheng, Lei, Wang, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909091/
https://www.ncbi.nlm.nih.gov/pubmed/24498071
http://dx.doi.org/10.1371/journal.pone.0087313
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author Hu, Yan-Wei
Zhang, Peng
Yang, Jun-Yao
Huang, Jin-Lan
Ma, Xin
Li, Shu-Fen
Zhao, Jia-Yi
Hu, Ya-Rong
Wang, Yan-Chao
Gao, Ji-Juan
Sha, Yan-Hua
Zheng, Lei
Wang, Qian
author_facet Hu, Yan-Wei
Zhang, Peng
Yang, Jun-Yao
Huang, Jin-Lan
Ma, Xin
Li, Shu-Fen
Zhao, Jia-Yi
Hu, Ya-Rong
Wang, Yan-Chao
Gao, Ji-Juan
Sha, Yan-Hua
Zheng, Lei
Wang, Qian
author_sort Hu, Yan-Wei
collection PubMed
description RATIONALE: It is clear that lipid disorder and inflammation are associated with cardiovascular diseases and underlying atherosclerosis. Nur77 has been shown to be involved in inflammatory response and lipid metabolism. OBJECTIVE: Here, we explored the role of Nur77 in atherosclerotic plaque progression in apoE(−/−) mice fed a high-fat/high cholesterol diet. METHODS AND RESULTS: The Nur77 gene, a nuclear hormone receptor, was highly induced by treatment with Cytosporone B (Csn-B, specific Nur77 agonist), recombinant plasmid over-expressing Nur77 (pcDNA-Nur77), while inhibited by treatment with siRNAs against Nur77 (si-Nur77) in THP-1 macrophage-derived foam cells, HepG2 cells and Caco-2 cells, respectively. In addition, the expression of Nur77 was highly induced by Nur77 agonist Csn-B, lentivirus encoding Nur77 (LV-Nur77), while silenced by lentivirus encoding siRNA against Nur77 (si-Nur77) in apoE(−/−) mice fed a high-fat/high cholesterol diet, respectively. We found that increased expression of Nur77 reduced macrophage-derived foam cells formation and hepatic lipid deposition, downregulated gene levels of inflammatory molecules, adhesion molecules and intestinal lipid absorption, and decreases atherosclerotic plaque formation. CONCLUSION: These observations provide direct evidence that Nur77 is an important nuclear hormone receptor in regulation of atherosclerotic plaque formation and thus represents a promising target for the treatment of atherosclerosis.
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spelling pubmed-39090912014-02-04 Nur77 Decreases Atherosclerosis Progression in apoE(−/−) Mice Fed a High-Fat/High-Cholesterol Diet Hu, Yan-Wei Zhang, Peng Yang, Jun-Yao Huang, Jin-Lan Ma, Xin Li, Shu-Fen Zhao, Jia-Yi Hu, Ya-Rong Wang, Yan-Chao Gao, Ji-Juan Sha, Yan-Hua Zheng, Lei Wang, Qian PLoS One Research Article RATIONALE: It is clear that lipid disorder and inflammation are associated with cardiovascular diseases and underlying atherosclerosis. Nur77 has been shown to be involved in inflammatory response and lipid metabolism. OBJECTIVE: Here, we explored the role of Nur77 in atherosclerotic plaque progression in apoE(−/−) mice fed a high-fat/high cholesterol diet. METHODS AND RESULTS: The Nur77 gene, a nuclear hormone receptor, was highly induced by treatment with Cytosporone B (Csn-B, specific Nur77 agonist), recombinant plasmid over-expressing Nur77 (pcDNA-Nur77), while inhibited by treatment with siRNAs against Nur77 (si-Nur77) in THP-1 macrophage-derived foam cells, HepG2 cells and Caco-2 cells, respectively. In addition, the expression of Nur77 was highly induced by Nur77 agonist Csn-B, lentivirus encoding Nur77 (LV-Nur77), while silenced by lentivirus encoding siRNA against Nur77 (si-Nur77) in apoE(−/−) mice fed a high-fat/high cholesterol diet, respectively. We found that increased expression of Nur77 reduced macrophage-derived foam cells formation and hepatic lipid deposition, downregulated gene levels of inflammatory molecules, adhesion molecules and intestinal lipid absorption, and decreases atherosclerotic plaque formation. CONCLUSION: These observations provide direct evidence that Nur77 is an important nuclear hormone receptor in regulation of atherosclerotic plaque formation and thus represents a promising target for the treatment of atherosclerosis. Public Library of Science 2014-01-31 /pmc/articles/PMC3909091/ /pubmed/24498071 http://dx.doi.org/10.1371/journal.pone.0087313 Text en © 2014 Hu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hu, Yan-Wei
Zhang, Peng
Yang, Jun-Yao
Huang, Jin-Lan
Ma, Xin
Li, Shu-Fen
Zhao, Jia-Yi
Hu, Ya-Rong
Wang, Yan-Chao
Gao, Ji-Juan
Sha, Yan-Hua
Zheng, Lei
Wang, Qian
Nur77 Decreases Atherosclerosis Progression in apoE(−/−) Mice Fed a High-Fat/High-Cholesterol Diet
title Nur77 Decreases Atherosclerosis Progression in apoE(−/−) Mice Fed a High-Fat/High-Cholesterol Diet
title_full Nur77 Decreases Atherosclerosis Progression in apoE(−/−) Mice Fed a High-Fat/High-Cholesterol Diet
title_fullStr Nur77 Decreases Atherosclerosis Progression in apoE(−/−) Mice Fed a High-Fat/High-Cholesterol Diet
title_full_unstemmed Nur77 Decreases Atherosclerosis Progression in apoE(−/−) Mice Fed a High-Fat/High-Cholesterol Diet
title_short Nur77 Decreases Atherosclerosis Progression in apoE(−/−) Mice Fed a High-Fat/High-Cholesterol Diet
title_sort nur77 decreases atherosclerosis progression in apoe(−/−) mice fed a high-fat/high-cholesterol diet
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909091/
https://www.ncbi.nlm.nih.gov/pubmed/24498071
http://dx.doi.org/10.1371/journal.pone.0087313
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