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Biochemical, Stabilization and Crystallization Studies on a Molecular Chaperone (PaoD) Involved in the Maturation of Molybdoenzymes
Molybdenum and tungsten enzymes require specific chaperones for folding and cofactor insertion. PaoD is the chaperone of the periplasmic aldehyde oxidoreductase PaoABC. It is the last gene in the paoABCD operon in Escherichia coli and its presence is crucial for obtaining mature enzyme. PaoD is an u...
Autores principales: | Otrelo-Cardoso, Ana Rita, Schwuchow, Viola, Rodrigues, David, Cabrita, Eurico J., Leimkühler, Silke, Romão, Maria João, Santos-Silva, Teresa |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909100/ https://www.ncbi.nlm.nih.gov/pubmed/24498065 http://dx.doi.org/10.1371/journal.pone.0087295 |
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