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Arp2/3 Complex Inhibition Prevents Meiotic Maturation in Porcine Oocytes

The Arp2/3 complex regulates actin nucleation, which is critical for a wide range of cellular processes, such as cell polarity, cell locomotion, and endocytosis. In the present study, we investigated the possible roles of the Arp2/3 complex in porcine oocytes during meiotic maturation. Immunofluores...

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Detalles Bibliográficos
Autores principales: Wang, Fei, An, Ga-Young, Zhang, Yu, Liu, Hong-Lin, Cui, Xiang-Shun, Kim, Nam-Hyung, Sun, Shao-Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909225/
https://www.ncbi.nlm.nih.gov/pubmed/24498171
http://dx.doi.org/10.1371/journal.pone.0087700
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author Wang, Fei
An, Ga-Young
Zhang, Yu
Liu, Hong-Lin
Cui, Xiang-Shun
Kim, Nam-Hyung
Sun, Shao-Chen
author_facet Wang, Fei
An, Ga-Young
Zhang, Yu
Liu, Hong-Lin
Cui, Xiang-Shun
Kim, Nam-Hyung
Sun, Shao-Chen
author_sort Wang, Fei
collection PubMed
description The Arp2/3 complex regulates actin nucleation, which is critical for a wide range of cellular processes, such as cell polarity, cell locomotion, and endocytosis. In the present study, we investigated the possible roles of the Arp2/3 complex in porcine oocytes during meiotic maturation. Immunofluorescent staining showed the Arp2/3 complex to localize mainly to the cortex of porcine oocytes, colocalizing with actin. Treatment with an Arp2/3 complex specific inhibitor, CK666, resulted in a decrease in Arp2/3 complex localization at the oocyte cortex. The maturation rate of porcine oocytes decreased significantly after CK666 treatment, concomitant with the failure of cumulus cell expansion and oocyte polar body extrusion. The fluorescence intensity of F-actin decreased in the cytoplasm, and CK666 also disrupted actin cap formation. In summary, our results illustrate that the Arp2/3 complex is required for the meiotic maturation of porcine oocytes and that actin nucleation is critical for meiotic maturation.
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spelling pubmed-39092252014-02-04 Arp2/3 Complex Inhibition Prevents Meiotic Maturation in Porcine Oocytes Wang, Fei An, Ga-Young Zhang, Yu Liu, Hong-Lin Cui, Xiang-Shun Kim, Nam-Hyung Sun, Shao-Chen PLoS One Research Article The Arp2/3 complex regulates actin nucleation, which is critical for a wide range of cellular processes, such as cell polarity, cell locomotion, and endocytosis. In the present study, we investigated the possible roles of the Arp2/3 complex in porcine oocytes during meiotic maturation. Immunofluorescent staining showed the Arp2/3 complex to localize mainly to the cortex of porcine oocytes, colocalizing with actin. Treatment with an Arp2/3 complex specific inhibitor, CK666, resulted in a decrease in Arp2/3 complex localization at the oocyte cortex. The maturation rate of porcine oocytes decreased significantly after CK666 treatment, concomitant with the failure of cumulus cell expansion and oocyte polar body extrusion. The fluorescence intensity of F-actin decreased in the cytoplasm, and CK666 also disrupted actin cap formation. In summary, our results illustrate that the Arp2/3 complex is required for the meiotic maturation of porcine oocytes and that actin nucleation is critical for meiotic maturation. Public Library of Science 2014-01-31 /pmc/articles/PMC3909225/ /pubmed/24498171 http://dx.doi.org/10.1371/journal.pone.0087700 Text en © 2014 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Fei
An, Ga-Young
Zhang, Yu
Liu, Hong-Lin
Cui, Xiang-Shun
Kim, Nam-Hyung
Sun, Shao-Chen
Arp2/3 Complex Inhibition Prevents Meiotic Maturation in Porcine Oocytes
title Arp2/3 Complex Inhibition Prevents Meiotic Maturation in Porcine Oocytes
title_full Arp2/3 Complex Inhibition Prevents Meiotic Maturation in Porcine Oocytes
title_fullStr Arp2/3 Complex Inhibition Prevents Meiotic Maturation in Porcine Oocytes
title_full_unstemmed Arp2/3 Complex Inhibition Prevents Meiotic Maturation in Porcine Oocytes
title_short Arp2/3 Complex Inhibition Prevents Meiotic Maturation in Porcine Oocytes
title_sort arp2/3 complex inhibition prevents meiotic maturation in porcine oocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909225/
https://www.ncbi.nlm.nih.gov/pubmed/24498171
http://dx.doi.org/10.1371/journal.pone.0087700
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