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Lactate Regulates Rat Male Germ Cell Function through Reactive Oxygen Species

Besides giving structural support, Sertoli cells regulate the fate of germ cells by supplying a variety of factors. These factors include hormones, several pro- and anti-apoptotic agents and also energetic substrates. Lactate is one of the compounds produced by Sertoli cells, which is utilized as an...

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Autores principales: Galardo, María Noel, Regueira, Mariana, Riera, María Fernanda, Pellizzari, Eliana Herminia, Cigorraga, Selva Beatriz, Meroni, Silvina Beatriz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909278/
https://www.ncbi.nlm.nih.gov/pubmed/24498241
http://dx.doi.org/10.1371/journal.pone.0088024
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author Galardo, María Noel
Regueira, Mariana
Riera, María Fernanda
Pellizzari, Eliana Herminia
Cigorraga, Selva Beatriz
Meroni, Silvina Beatriz
author_facet Galardo, María Noel
Regueira, Mariana
Riera, María Fernanda
Pellizzari, Eliana Herminia
Cigorraga, Selva Beatriz
Meroni, Silvina Beatriz
author_sort Galardo, María Noel
collection PubMed
description Besides giving structural support, Sertoli cells regulate the fate of germ cells by supplying a variety of factors. These factors include hormones, several pro- and anti-apoptotic agents and also energetic substrates. Lactate is one of the compounds produced by Sertoli cells, which is utilized as an energetic substrate by germ cells, particularly spermatocytes and spermatids. Beyond its function as an energy source, some studies have proposed a role of lactate in the regulation of gene expression not strictly related to the energetic state of the cells. The general hypothesis that motivated this investigation was that lactate affects male germ cell function, far beyond its well-known role as energetic substrate. To evaluate this hypothesis we investigated: 1) if lactate was able to regulate germ cell gene expression and if reactive oxygen species (ROS) participated in this regulation, 2) if different signal transduction pathways were modified by the production of ROS in response to lactate and 3) possible mechanisms that may be involved in lactate stimulation of ROS production. In order to achieve these goals, cultures of germ cells obtained from male 30-day old rats were exposed to 10 or 20 mM lactate. Increases in lactate dehydrogenase (LDH) C and monocarboxylate transporter (MCT)2 expression, in Akt and p38-MAPK phosphorylation levels and in ROS production were observed. These effects were impaired in the presence of a ROS scavenger. Lactate stimulated ROS production was also inhibited by a LDH inhibitor or a NAD(P)H oxidase (NOX) inhibitor. NOX4 expression was identified in male germ cells. The results obtained herein are consistent with a scenario where lactate, taken up by germ cells, becomes oxidized to pyruvate with the resultant increase in NADH, which is a substrate for NOX4. ROS, products of NOX4 activity, may act as second messengers regulating signal transduction pathways and gene expression.
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spelling pubmed-39092782014-02-04 Lactate Regulates Rat Male Germ Cell Function through Reactive Oxygen Species Galardo, María Noel Regueira, Mariana Riera, María Fernanda Pellizzari, Eliana Herminia Cigorraga, Selva Beatriz Meroni, Silvina Beatriz PLoS One Research Article Besides giving structural support, Sertoli cells regulate the fate of germ cells by supplying a variety of factors. These factors include hormones, several pro- and anti-apoptotic agents and also energetic substrates. Lactate is one of the compounds produced by Sertoli cells, which is utilized as an energetic substrate by germ cells, particularly spermatocytes and spermatids. Beyond its function as an energy source, some studies have proposed a role of lactate in the regulation of gene expression not strictly related to the energetic state of the cells. The general hypothesis that motivated this investigation was that lactate affects male germ cell function, far beyond its well-known role as energetic substrate. To evaluate this hypothesis we investigated: 1) if lactate was able to regulate germ cell gene expression and if reactive oxygen species (ROS) participated in this regulation, 2) if different signal transduction pathways were modified by the production of ROS in response to lactate and 3) possible mechanisms that may be involved in lactate stimulation of ROS production. In order to achieve these goals, cultures of germ cells obtained from male 30-day old rats were exposed to 10 or 20 mM lactate. Increases in lactate dehydrogenase (LDH) C and monocarboxylate transporter (MCT)2 expression, in Akt and p38-MAPK phosphorylation levels and in ROS production were observed. These effects were impaired in the presence of a ROS scavenger. Lactate stimulated ROS production was also inhibited by a LDH inhibitor or a NAD(P)H oxidase (NOX) inhibitor. NOX4 expression was identified in male germ cells. The results obtained herein are consistent with a scenario where lactate, taken up by germ cells, becomes oxidized to pyruvate with the resultant increase in NADH, which is a substrate for NOX4. ROS, products of NOX4 activity, may act as second messengers regulating signal transduction pathways and gene expression. Public Library of Science 2014-01-31 /pmc/articles/PMC3909278/ /pubmed/24498241 http://dx.doi.org/10.1371/journal.pone.0088024 Text en © 2014 Galardo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Galardo, María Noel
Regueira, Mariana
Riera, María Fernanda
Pellizzari, Eliana Herminia
Cigorraga, Selva Beatriz
Meroni, Silvina Beatriz
Lactate Regulates Rat Male Germ Cell Function through Reactive Oxygen Species
title Lactate Regulates Rat Male Germ Cell Function through Reactive Oxygen Species
title_full Lactate Regulates Rat Male Germ Cell Function through Reactive Oxygen Species
title_fullStr Lactate Regulates Rat Male Germ Cell Function through Reactive Oxygen Species
title_full_unstemmed Lactate Regulates Rat Male Germ Cell Function through Reactive Oxygen Species
title_short Lactate Regulates Rat Male Germ Cell Function through Reactive Oxygen Species
title_sort lactate regulates rat male germ cell function through reactive oxygen species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909278/
https://www.ncbi.nlm.nih.gov/pubmed/24498241
http://dx.doi.org/10.1371/journal.pone.0088024
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