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Dividing Cells Regulate Their Lipid Composition and Localization

Although massive membrane rearrangements occur during cell division, little is known about specific roles that lipids might play in this process. We report that the lipidome changes with the cell cycle. LC-MS-based lipid profiling shows that 11 lipids with specific chemical structures accumulate in...

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Autores principales: Atilla-Gokcumen, G. Ekin, Muro, Eleonora, Relat-Goberna, Josep, Sasse, Sofia, Bedigian, Anne, Coughlin, Margaret L., Garcia-Manyes, Sergi, Eggert, Ulrike S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909459/
https://www.ncbi.nlm.nih.gov/pubmed/24462247
http://dx.doi.org/10.1016/j.cell.2013.12.015
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author Atilla-Gokcumen, G. Ekin
Muro, Eleonora
Relat-Goberna, Josep
Sasse, Sofia
Bedigian, Anne
Coughlin, Margaret L.
Garcia-Manyes, Sergi
Eggert, Ulrike S.
author_facet Atilla-Gokcumen, G. Ekin
Muro, Eleonora
Relat-Goberna, Josep
Sasse, Sofia
Bedigian, Anne
Coughlin, Margaret L.
Garcia-Manyes, Sergi
Eggert, Ulrike S.
author_sort Atilla-Gokcumen, G. Ekin
collection PubMed
description Although massive membrane rearrangements occur during cell division, little is known about specific roles that lipids might play in this process. We report that the lipidome changes with the cell cycle. LC-MS-based lipid profiling shows that 11 lipids with specific chemical structures accumulate in dividing cells. Using AFM, we demonstrate differences in the mechanical properties of live dividing cells and their isolated lipids relative to nondividing cells. In parallel, systematic RNAi knockdown of lipid biosynthetic enzymes identified enzymes required for division, which highly correlated with lipids accumulated in dividing cells. We show that cells specifically regulate the localization of lipids to midbodies, membrane-based structures where cleavage occurs. We conclude that cells actively regulate and modulate their lipid composition and localization during division, with both signaling and structural roles likely. This work has broader implications for the active and sustained participation of lipids in basic biology.
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spelling pubmed-39094592014-02-03 Dividing Cells Regulate Their Lipid Composition and Localization Atilla-Gokcumen, G. Ekin Muro, Eleonora Relat-Goberna, Josep Sasse, Sofia Bedigian, Anne Coughlin, Margaret L. Garcia-Manyes, Sergi Eggert, Ulrike S. Cell Article Although massive membrane rearrangements occur during cell division, little is known about specific roles that lipids might play in this process. We report that the lipidome changes with the cell cycle. LC-MS-based lipid profiling shows that 11 lipids with specific chemical structures accumulate in dividing cells. Using AFM, we demonstrate differences in the mechanical properties of live dividing cells and their isolated lipids relative to nondividing cells. In parallel, systematic RNAi knockdown of lipid biosynthetic enzymes identified enzymes required for division, which highly correlated with lipids accumulated in dividing cells. We show that cells specifically regulate the localization of lipids to midbodies, membrane-based structures where cleavage occurs. We conclude that cells actively regulate and modulate their lipid composition and localization during division, with both signaling and structural roles likely. This work has broader implications for the active and sustained participation of lipids in basic biology. Cell Press 2014-01-30 /pmc/articles/PMC3909459/ /pubmed/24462247 http://dx.doi.org/10.1016/j.cell.2013.12.015 Text en © 2014 The Authors https://creativecommons.org/licenses/by/3.0/This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Atilla-Gokcumen, G. Ekin
Muro, Eleonora
Relat-Goberna, Josep
Sasse, Sofia
Bedigian, Anne
Coughlin, Margaret L.
Garcia-Manyes, Sergi
Eggert, Ulrike S.
Dividing Cells Regulate Their Lipid Composition and Localization
title Dividing Cells Regulate Their Lipid Composition and Localization
title_full Dividing Cells Regulate Their Lipid Composition and Localization
title_fullStr Dividing Cells Regulate Their Lipid Composition and Localization
title_full_unstemmed Dividing Cells Regulate Their Lipid Composition and Localization
title_short Dividing Cells Regulate Their Lipid Composition and Localization
title_sort dividing cells regulate their lipid composition and localization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909459/
https://www.ncbi.nlm.nih.gov/pubmed/24462247
http://dx.doi.org/10.1016/j.cell.2013.12.015
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