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Host biomarkers distinguish dengue from leptospirosis in Colombia: a case–control study

BACKGROUND: Dengue fever and leptospirosis have partially overlapping geographic distributions, similar clinical presentations and potentially life-threatening complications but require different treatments. Distinguishing between these cosmopolitan emerging pathogens represents a diagnostic dilemma...

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Autores principales: Conroy, Andrea L, Gélvez, Margarita, Hawkes, Michael, Rajwans, Nimerta, Liles, W Conrad, Villar-Centeno, Luis Angel, Kain, Kevin C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909480/
https://www.ncbi.nlm.nih.gov/pubmed/24444080
http://dx.doi.org/10.1186/1471-2334-14-35
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author Conroy, Andrea L
Gélvez, Margarita
Hawkes, Michael
Rajwans, Nimerta
Liles, W Conrad
Villar-Centeno, Luis Angel
Kain, Kevin C
author_facet Conroy, Andrea L
Gélvez, Margarita
Hawkes, Michael
Rajwans, Nimerta
Liles, W Conrad
Villar-Centeno, Luis Angel
Kain, Kevin C
author_sort Conroy, Andrea L
collection PubMed
description BACKGROUND: Dengue fever and leptospirosis have partially overlapping geographic distributions, similar clinical presentations and potentially life-threatening complications but require different treatments. Distinguishing between these cosmopolitan emerging pathogens represents a diagnostic dilemma of global importance. We hypothesized that perturbations in host biomarkers can differentiate between individuals with dengue fever and leptospirosis during the acute phase of illness. METHODS: We randomly selected subjects from a prospective cohort study of acute febrile illness in Bucaramanga, Colombia and tested 19 serum biomarkers by ELISA in dengue fever (DF, n = 113) compared to subjects with leptospirosis (n = 47). Biomarkers were selected for further analysis if they had good discriminatory ability (area under the ROC curve (AUC) >0.80) and were beyond a reference range (assessed using local healthy controls). RESULTS: Nine biomarkers differed significantly between dengue fever and leptospirosis, with higher levels of Angptl3, IL-18BP, IP-10/CXCL10, Platelet Factor 4, sICAM-1, Factor D, sEng and sKDR in dengue and higher levels of sTie-2 in leptospirosis (p < 0.001 for all comparisons). Two biomarkers, sEng and IL18BP, showed excellent discriminatory ability (AUROC >0.90). When incorporated into multivariable models, sEng and IL18BP improved the diagnostic accuracy of clinical information alone. CONCLUSIONS: These results suggest that host biomarkers may have utility in differentiating between dengue and leptospirosis, clinically similar conditions of different etiology.
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spelling pubmed-39094802014-02-02 Host biomarkers distinguish dengue from leptospirosis in Colombia: a case–control study Conroy, Andrea L Gélvez, Margarita Hawkes, Michael Rajwans, Nimerta Liles, W Conrad Villar-Centeno, Luis Angel Kain, Kevin C BMC Infect Dis Research Article BACKGROUND: Dengue fever and leptospirosis have partially overlapping geographic distributions, similar clinical presentations and potentially life-threatening complications but require different treatments. Distinguishing between these cosmopolitan emerging pathogens represents a diagnostic dilemma of global importance. We hypothesized that perturbations in host biomarkers can differentiate between individuals with dengue fever and leptospirosis during the acute phase of illness. METHODS: We randomly selected subjects from a prospective cohort study of acute febrile illness in Bucaramanga, Colombia and tested 19 serum biomarkers by ELISA in dengue fever (DF, n = 113) compared to subjects with leptospirosis (n = 47). Biomarkers were selected for further analysis if they had good discriminatory ability (area under the ROC curve (AUC) >0.80) and were beyond a reference range (assessed using local healthy controls). RESULTS: Nine biomarkers differed significantly between dengue fever and leptospirosis, with higher levels of Angptl3, IL-18BP, IP-10/CXCL10, Platelet Factor 4, sICAM-1, Factor D, sEng and sKDR in dengue and higher levels of sTie-2 in leptospirosis (p < 0.001 for all comparisons). Two biomarkers, sEng and IL18BP, showed excellent discriminatory ability (AUROC >0.90). When incorporated into multivariable models, sEng and IL18BP improved the diagnostic accuracy of clinical information alone. CONCLUSIONS: These results suggest that host biomarkers may have utility in differentiating between dengue and leptospirosis, clinically similar conditions of different etiology. BioMed Central 2014-01-20 /pmc/articles/PMC3909480/ /pubmed/24444080 http://dx.doi.org/10.1186/1471-2334-14-35 Text en Copyright © 2014 Conroy et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Conroy, Andrea L
Gélvez, Margarita
Hawkes, Michael
Rajwans, Nimerta
Liles, W Conrad
Villar-Centeno, Luis Angel
Kain, Kevin C
Host biomarkers distinguish dengue from leptospirosis in Colombia: a case–control study
title Host biomarkers distinguish dengue from leptospirosis in Colombia: a case–control study
title_full Host biomarkers distinguish dengue from leptospirosis in Colombia: a case–control study
title_fullStr Host biomarkers distinguish dengue from leptospirosis in Colombia: a case–control study
title_full_unstemmed Host biomarkers distinguish dengue from leptospirosis in Colombia: a case–control study
title_short Host biomarkers distinguish dengue from leptospirosis in Colombia: a case–control study
title_sort host biomarkers distinguish dengue from leptospirosis in colombia: a case–control study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909480/
https://www.ncbi.nlm.nih.gov/pubmed/24444080
http://dx.doi.org/10.1186/1471-2334-14-35
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