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Drug-Resistance Associated Mutations in Polymerase (P) Gene of Hepatitis B Virus Isolated From Malaysian HBV Carriers

BACKGROUND: Mutations in the polymerase (P) gene of hepatitis B virus are often associated with drug resistance. The pattern of mutations varies geographically, thus giving rise to genotypes diversity. OBJECTIVES: This study was carried out to detect mutations in P gene of hepatitis B virus isolated...

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Detalles Bibliográficos
Autores principales: Suppiah, Jeyanthi, Mohd Zain, Rozainanee, Haji Nawi, Salbiah, Bahari, Norazlah, Saat, Zainah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909636/
https://www.ncbi.nlm.nih.gov/pubmed/24497877
http://dx.doi.org/10.5812/hepatmon.13173
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author Suppiah, Jeyanthi
Mohd Zain, Rozainanee
Haji Nawi, Salbiah
Bahari, Norazlah
Saat, Zainah
author_facet Suppiah, Jeyanthi
Mohd Zain, Rozainanee
Haji Nawi, Salbiah
Bahari, Norazlah
Saat, Zainah
author_sort Suppiah, Jeyanthi
collection PubMed
description BACKGROUND: Mutations in the polymerase (P) gene of hepatitis B virus are often associated with drug resistance. The pattern of mutations varies geographically, thus giving rise to genotypes diversity. OBJECTIVES: This study was carried out to detect mutations in P gene of hepatitis B virus isolated from Malaysian HBV carriers. MATERIALS AND METHODS: A total of 58 sera samples were analyzed by PCR and sequencing, of which the P gene of isolated HBV was successfully amplified and sequenced from 40 samples. RESULTS: Genotyping of these samples revealed that the predominant genotype was genotype C (22/40, 55.0%), followed by genotype B (17/40, 42.5%), and only 1 sample showed genotype D (2.5%). A number of significant drug resistant mutations were found in five patients including S202I, N236T, M250L, L180M/V, M204I, A181T, T184G, M250V, and V173L. Of these, L180M/V and M204I were most frequently detected (80%) and associated with lamivudine in combination with emtricitabine and telbivudine drug resistance. Association with age, sex, and clinical symptoms revealed that these patients were all male, mid to elderly age and almost all hadcirrhotic liver disease. CONCLUSIONS: Detection and surveillance of the significant sites of mutations in HBV is crucial for clinicians to decide on the choice of antiviral treatment and further management of hepatitis B carriers.
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spelling pubmed-39096362014-02-04 Drug-Resistance Associated Mutations in Polymerase (P) Gene of Hepatitis B Virus Isolated From Malaysian HBV Carriers Suppiah, Jeyanthi Mohd Zain, Rozainanee Haji Nawi, Salbiah Bahari, Norazlah Saat, Zainah Hepat Mon Research Article BACKGROUND: Mutations in the polymerase (P) gene of hepatitis B virus are often associated with drug resistance. The pattern of mutations varies geographically, thus giving rise to genotypes diversity. OBJECTIVES: This study was carried out to detect mutations in P gene of hepatitis B virus isolated from Malaysian HBV carriers. MATERIALS AND METHODS: A total of 58 sera samples were analyzed by PCR and sequencing, of which the P gene of isolated HBV was successfully amplified and sequenced from 40 samples. RESULTS: Genotyping of these samples revealed that the predominant genotype was genotype C (22/40, 55.0%), followed by genotype B (17/40, 42.5%), and only 1 sample showed genotype D (2.5%). A number of significant drug resistant mutations were found in five patients including S202I, N236T, M250L, L180M/V, M204I, A181T, T184G, M250V, and V173L. Of these, L180M/V and M204I were most frequently detected (80%) and associated with lamivudine in combination with emtricitabine and telbivudine drug resistance. Association with age, sex, and clinical symptoms revealed that these patients were all male, mid to elderly age and almost all hadcirrhotic liver disease. CONCLUSIONS: Detection and surveillance of the significant sites of mutations in HBV is crucial for clinicians to decide on the choice of antiviral treatment and further management of hepatitis B carriers. Kowsar 2014-01-05 /pmc/articles/PMC3909636/ /pubmed/24497877 http://dx.doi.org/10.5812/hepatmon.13173 Text en Copyright © 2014, BRCGL. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Suppiah, Jeyanthi
Mohd Zain, Rozainanee
Haji Nawi, Salbiah
Bahari, Norazlah
Saat, Zainah
Drug-Resistance Associated Mutations in Polymerase (P) Gene of Hepatitis B Virus Isolated From Malaysian HBV Carriers
title Drug-Resistance Associated Mutations in Polymerase (P) Gene of Hepatitis B Virus Isolated From Malaysian HBV Carriers
title_full Drug-Resistance Associated Mutations in Polymerase (P) Gene of Hepatitis B Virus Isolated From Malaysian HBV Carriers
title_fullStr Drug-Resistance Associated Mutations in Polymerase (P) Gene of Hepatitis B Virus Isolated From Malaysian HBV Carriers
title_full_unstemmed Drug-Resistance Associated Mutations in Polymerase (P) Gene of Hepatitis B Virus Isolated From Malaysian HBV Carriers
title_short Drug-Resistance Associated Mutations in Polymerase (P) Gene of Hepatitis B Virus Isolated From Malaysian HBV Carriers
title_sort drug-resistance associated mutations in polymerase (p) gene of hepatitis b virus isolated from malaysian hbv carriers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909636/
https://www.ncbi.nlm.nih.gov/pubmed/24497877
http://dx.doi.org/10.5812/hepatmon.13173
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