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Nitrosopersulfide (SSNO(−)) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide()

Sulfide salts are known to promote the release of nitric oxide (NO) from S-nitrosothiols and potentiate their vasorelaxant activity, but much of the cross-talk between hydrogen sulfide and NO is believed to occur via functional interactions of cell regulatory elements such as phosphodiesterases. Usi...

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Autores principales: Cortese-Krott, Miriam M., Fernandez, Bernadette O., Santos, José L.T., Mergia, Evanthia, Grman, Marian, Nagy, Péter, Kelm, Malte, Butler, Anthony, Feelisch, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909780/
https://www.ncbi.nlm.nih.gov/pubmed/24494198
http://dx.doi.org/10.1016/j.redox.2013.12.031
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author Cortese-Krott, Miriam M.
Fernandez, Bernadette O.
Santos, José L.T.
Mergia, Evanthia
Grman, Marian
Nagy, Péter
Kelm, Malte
Butler, Anthony
Feelisch, Martin
author_facet Cortese-Krott, Miriam M.
Fernandez, Bernadette O.
Santos, José L.T.
Mergia, Evanthia
Grman, Marian
Nagy, Péter
Kelm, Malte
Butler, Anthony
Feelisch, Martin
author_sort Cortese-Krott, Miriam M.
collection PubMed
description Sulfide salts are known to promote the release of nitric oxide (NO) from S-nitrosothiols and potentiate their vasorelaxant activity, but much of the cross-talk between hydrogen sulfide and NO is believed to occur via functional interactions of cell regulatory elements such as phosphodiesterases. Using RFL-6 cells as an NO reporter system we sought to investigate whether sulfide can also modulate nitrosothiol-mediated soluble guanylyl cyclase (sGC) activation following direct chemical interaction. We find a U-shaped dose response relationship where low sulfide concentrations attenuate sGC stimulation by S-nitrosopenicillamine (SNAP) and cyclic GMP levels are restored at equimolar ratios. Similar results are observed when intracellular sulfide levels are raised by pre-incubation with the sulfide donor, GYY4137. The outcome of direct sulfide/nitrosothiol interactions also critically depends on molar reactant ratios and is accompanied by oxygen consumption. With sulfide in excess, a ‘yellow compound’ accumulates that is indistinguishable from the product of solid-phase transnitrosation of either hydrosulfide or hydrodisulfide and assigned to be nitrosopersulfide (perthionitrite, SSNO(−); λ(max) 412 nm in aqueous buffers, pH 7.4; 448 nm in DMF). Time-resolved chemiluminescence and UV–visible spectroscopy analyses suggest that its generation is preceded by formation of the short-lived NO-donor, thionitrite (SNO(−)). In contrast to the latter, SSNO(−) is rather stable at physiological pH and generates both NO and polysulfides on decomposition, resulting in sustained potentiation of SNAP-induced sGC stimulation. Thus, sulfide reacts with nitrosothiols to form multiple bioactive products; SSNO(−) rather than SNO(−) may account for some of the longer-lived effects of nitrosothiols and contribute to sulfide and NO signaling.
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spelling pubmed-39097802014-02-03 Nitrosopersulfide (SSNO(−)) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide() Cortese-Krott, Miriam M. Fernandez, Bernadette O. Santos, José L.T. Mergia, Evanthia Grman, Marian Nagy, Péter Kelm, Malte Butler, Anthony Feelisch, Martin Redox Biol Research Paper Sulfide salts are known to promote the release of nitric oxide (NO) from S-nitrosothiols and potentiate their vasorelaxant activity, but much of the cross-talk between hydrogen sulfide and NO is believed to occur via functional interactions of cell regulatory elements such as phosphodiesterases. Using RFL-6 cells as an NO reporter system we sought to investigate whether sulfide can also modulate nitrosothiol-mediated soluble guanylyl cyclase (sGC) activation following direct chemical interaction. We find a U-shaped dose response relationship where low sulfide concentrations attenuate sGC stimulation by S-nitrosopenicillamine (SNAP) and cyclic GMP levels are restored at equimolar ratios. Similar results are observed when intracellular sulfide levels are raised by pre-incubation with the sulfide donor, GYY4137. The outcome of direct sulfide/nitrosothiol interactions also critically depends on molar reactant ratios and is accompanied by oxygen consumption. With sulfide in excess, a ‘yellow compound’ accumulates that is indistinguishable from the product of solid-phase transnitrosation of either hydrosulfide or hydrodisulfide and assigned to be nitrosopersulfide (perthionitrite, SSNO(−); λ(max) 412 nm in aqueous buffers, pH 7.4; 448 nm in DMF). Time-resolved chemiluminescence and UV–visible spectroscopy analyses suggest that its generation is preceded by formation of the short-lived NO-donor, thionitrite (SNO(−)). In contrast to the latter, SSNO(−) is rather stable at physiological pH and generates both NO and polysulfides on decomposition, resulting in sustained potentiation of SNAP-induced sGC stimulation. Thus, sulfide reacts with nitrosothiols to form multiple bioactive products; SSNO(−) rather than SNO(−) may account for some of the longer-lived effects of nitrosothiols and contribute to sulfide and NO signaling. Elsevier 2014-01-11 /pmc/articles/PMC3909780/ /pubmed/24494198 http://dx.doi.org/10.1016/j.redox.2013.12.031 Text en © 2014 The Authors https://creativecommons.org/licenses/by/3.0/This work is licensed under a Creative Commons Attribution 3.0 Unported License (https://creativecommons.org/licenses/by/3.0/) .
spellingShingle Research Paper
Cortese-Krott, Miriam M.
Fernandez, Bernadette O.
Santos, José L.T.
Mergia, Evanthia
Grman, Marian
Nagy, Péter
Kelm, Malte
Butler, Anthony
Feelisch, Martin
Nitrosopersulfide (SSNO(−)) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide()
title Nitrosopersulfide (SSNO(−)) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide()
title_full Nitrosopersulfide (SSNO(−)) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide()
title_fullStr Nitrosopersulfide (SSNO(−)) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide()
title_full_unstemmed Nitrosopersulfide (SSNO(−)) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide()
title_short Nitrosopersulfide (SSNO(−)) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide()
title_sort nitrosopersulfide (ssno(−)) accounts for sustained no bioactivity of s-nitrosothiols following reaction with sulfide()
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909780/
https://www.ncbi.nlm.nih.gov/pubmed/24494198
http://dx.doi.org/10.1016/j.redox.2013.12.031
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