Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5()

Cytochrome P450 gene expression is altered by various chemical compounds. In this study, we used nuclear factor erythroid 2-related factor 2 (Nrf2)–deficient (Nrf2(−⧸−)) mice to investigate the involvement of Nrf2 in Cyp2b10 and Cyp2a5 gene expression. Phorone, an Nrf2 activator, strongly increased...

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Autores principales: Ashino, Takashi, Ohkubo-Morita, Haruyo, Yamamoto, Masayuki, Yoshida, Takemi, Numazawa, Satoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909825/
https://www.ncbi.nlm.nih.gov/pubmed/24494203
http://dx.doi.org/10.1016/j.redox.2013.12.025
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author Ashino, Takashi
Ohkubo-Morita, Haruyo
Yamamoto, Masayuki
Yoshida, Takemi
Numazawa, Satoshi
author_facet Ashino, Takashi
Ohkubo-Morita, Haruyo
Yamamoto, Masayuki
Yoshida, Takemi
Numazawa, Satoshi
author_sort Ashino, Takashi
collection PubMed
description Cytochrome P450 gene expression is altered by various chemical compounds. In this study, we used nuclear factor erythroid 2-related factor 2 (Nrf2)–deficient (Nrf2(−⧸−)) mice to investigate the involvement of Nrf2 in Cyp2b10 and Cyp2a5 gene expression. Phorone, an Nrf2 activator, strongly increased Cyp2b10 and Cyp2a5 mRNA as well as Nrf2 target genes, including NAD(P)H-quinone oxidoreductase-1 and heme oxygenase-1, in wild-type mouse livers 8 h after treatment. The phorone-induced mRNA levels in Nrf2(−⧸−) mouse livers were lower than that in wild-type mouse livers. Nrf2(−⧸−) mice showed attenuated Cyp2b10 and Cyp2a5 induction by phenobarbital, a classical Cyp2b inducer. These findings suggest that the Nrf2 pathway is involved in Cyp2b10 and Cyp2a5 gene expression.
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spelling pubmed-39098252014-02-03 Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5() Ashino, Takashi Ohkubo-Morita, Haruyo Yamamoto, Masayuki Yoshida, Takemi Numazawa, Satoshi Redox Biol Short Communications Cytochrome P450 gene expression is altered by various chemical compounds. In this study, we used nuclear factor erythroid 2-related factor 2 (Nrf2)–deficient (Nrf2(−⧸−)) mice to investigate the involvement of Nrf2 in Cyp2b10 and Cyp2a5 gene expression. Phorone, an Nrf2 activator, strongly increased Cyp2b10 and Cyp2a5 mRNA as well as Nrf2 target genes, including NAD(P)H-quinone oxidoreductase-1 and heme oxygenase-1, in wild-type mouse livers 8 h after treatment. The phorone-induced mRNA levels in Nrf2(−⧸−) mouse livers were lower than that in wild-type mouse livers. Nrf2(−⧸−) mice showed attenuated Cyp2b10 and Cyp2a5 induction by phenobarbital, a classical Cyp2b inducer. These findings suggest that the Nrf2 pathway is involved in Cyp2b10 and Cyp2a5 gene expression. Elsevier 2014-01-10 /pmc/articles/PMC3909825/ /pubmed/24494203 http://dx.doi.org/10.1016/j.redox.2013.12.025 Text en © 2014 The Authors https://creativecommons.org/licenses/by-nc-sa/3.0/This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License (https://creativecommons.org/licenses/by-nc-sa/3.0/) .
spellingShingle Short Communications
Ashino, Takashi
Ohkubo-Morita, Haruyo
Yamamoto, Masayuki
Yoshida, Takemi
Numazawa, Satoshi
Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5()
title Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5()
title_full Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5()
title_fullStr Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5()
title_full_unstemmed Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5()
title_short Possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of Cyp2b10 and Cyp2a5()
title_sort possible involvement of nuclear factor erythroid 2-related factor 2 in the gene expression of cyp2b10 and cyp2a5()
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909825/
https://www.ncbi.nlm.nih.gov/pubmed/24494203
http://dx.doi.org/10.1016/j.redox.2013.12.025
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