Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system

Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive...

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Detalles Bibliográficos
Autores principales: Zhao, Yicheng, Dai, Zhen, Liang, Yang, Yin, Ming, Ma, Kuiying, He, Mei, Ouyang, Hongsheng, Teng, Chun-Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909901/
https://www.ncbi.nlm.nih.gov/pubmed/24487629
http://dx.doi.org/10.1038/srep03943
Descripción
Sumario:Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive Cas9 can reversibly prevent the expression of both monocistronic miRNAs and polycistronic miRNA clusters. Furthermore, CRISPR/CRISPRi is also capable of suppressing genes in porcine cells.

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