Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system

Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive...

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Detalles Bibliográficos
Autores principales: Zhao, Yicheng, Dai, Zhen, Liang, Yang, Yin, Ming, Ma, Kuiying, He, Mei, Ouyang, Hongsheng, Teng, Chun-Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909901/
https://www.ncbi.nlm.nih.gov/pubmed/24487629
http://dx.doi.org/10.1038/srep03943
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author Zhao, Yicheng
Dai, Zhen
Liang, Yang
Yin, Ming
Ma, Kuiying
He, Mei
Ouyang, Hongsheng
Teng, Chun-Bo
author_facet Zhao, Yicheng
Dai, Zhen
Liang, Yang
Yin, Ming
Ma, Kuiying
He, Mei
Ouyang, Hongsheng
Teng, Chun-Bo
author_sort Zhao, Yicheng
collection PubMed
description Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive Cas9 can reversibly prevent the expression of both monocistronic miRNAs and polycistronic miRNA clusters. Furthermore, CRISPR/CRISPRi is also capable of suppressing genes in porcine cells.
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spelling pubmed-39099012014-02-03 Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system Zhao, Yicheng Dai, Zhen Liang, Yang Yin, Ming Ma, Kuiying He, Mei Ouyang, Hongsheng Teng, Chun-Bo Sci Rep Article Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive Cas9 can reversibly prevent the expression of both monocistronic miRNAs and polycistronic miRNA clusters. Furthermore, CRISPR/CRISPRi is also capable of suppressing genes in porcine cells. Nature Publishing Group 2014-02-03 /pmc/articles/PMC3909901/ /pubmed/24487629 http://dx.doi.org/10.1038/srep03943 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Zhao, Yicheng
Dai, Zhen
Liang, Yang
Yin, Ming
Ma, Kuiying
He, Mei
Ouyang, Hongsheng
Teng, Chun-Bo
Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system
title Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system
title_full Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system
title_fullStr Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system
title_full_unstemmed Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system
title_short Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system
title_sort sequence-specific inhibition of microrna via crispr/crispri system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909901/
https://www.ncbi.nlm.nih.gov/pubmed/24487629
http://dx.doi.org/10.1038/srep03943
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