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Colicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea
Thirty one out of 153 strains of Shigella sonnei isolated from Thai patients with diarrhoea showed antibacterial activity against S. sonnei by agar well diffusion method. All of them harbor plasmids with the genetic determination of colicin type 7 (Js) gene but without colicin E and colicin U gene....
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Brazilian Society of Microbiology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910181/ https://www.ncbi.nlm.nih.gov/pubmed/24516440 |
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author | Kaewklom, Siriporn Samosornsuk, Seksun Pipatsatitpong, Duangnate Aunpad, Ratchaneewan |
author_facet | Kaewklom, Siriporn Samosornsuk, Seksun Pipatsatitpong, Duangnate Aunpad, Ratchaneewan |
author_sort | Kaewklom, Siriporn |
collection | PubMed |
description | Thirty one out of 153 strains of Shigella sonnei isolated from Thai patients with diarrhoea showed antibacterial activity against S. sonnei by agar well diffusion method. All of them harbor plasmids with the genetic determination of colicin type 7 (Js) gene but without colicin E and colicin U gene. The PCR product obtained from strain 35/44 was shown to be the gene for colicin type 7 lytic protein (cja). The partially purified bacteriocin (PPB) containing colicin type 7 of strain 35/44 was prepared and used for characterization. The antibacterial activity of PPB against a total of 17 selected Gram-positive and Gram-negative bacteria was tested. It was found that PPB of strain 35/44 was active against E. coli O157, S. sonnei and S. boydii. The sensitivity of PPB from this strain to proteinase K, trypsin and α-chymotrypsin suggests the proteinaceous nature of these antimicrobial substances. Therefore, this isolated bacterium can be regarded as bacteriocin producing bacteria. The bacteriocin produced by this isolated S. sonnei was heat stable as evidenced by its ability to maintain the activity at 80 °C for 60 min. In addition, it was stable within a wide range of pH (3–9). The molecular weight of colicin type 7 from isolated S. sonnei strain 35/44 analyzed by SDS-PAGE was 54.4 kDa composing of at least five subunits. It is to our knowledge; the first report of Thai patients with diarrhoea that S. sonnei isolated from them contained colicin type 7. |
format | Online Article Text |
id | pubmed-3910181 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Brazilian Society of Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-39101812014-02-15 Colicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea Kaewklom, Siriporn Samosornsuk, Seksun Pipatsatitpong, Duangnate Aunpad, Ratchaneewan Braz J Microbiol Research Paper Thirty one out of 153 strains of Shigella sonnei isolated from Thai patients with diarrhoea showed antibacterial activity against S. sonnei by agar well diffusion method. All of them harbor plasmids with the genetic determination of colicin type 7 (Js) gene but without colicin E and colicin U gene. The PCR product obtained from strain 35/44 was shown to be the gene for colicin type 7 lytic protein (cja). The partially purified bacteriocin (PPB) containing colicin type 7 of strain 35/44 was prepared and used for characterization. The antibacterial activity of PPB against a total of 17 selected Gram-positive and Gram-negative bacteria was tested. It was found that PPB of strain 35/44 was active against E. coli O157, S. sonnei and S. boydii. The sensitivity of PPB from this strain to proteinase K, trypsin and α-chymotrypsin suggests the proteinaceous nature of these antimicrobial substances. Therefore, this isolated bacterium can be regarded as bacteriocin producing bacteria. The bacteriocin produced by this isolated S. sonnei was heat stable as evidenced by its ability to maintain the activity at 80 °C for 60 min. In addition, it was stable within a wide range of pH (3–9). The molecular weight of colicin type 7 from isolated S. sonnei strain 35/44 analyzed by SDS-PAGE was 54.4 kDa composing of at least five subunits. It is to our knowledge; the first report of Thai patients with diarrhoea that S. sonnei isolated from them contained colicin type 7. Brazilian Society of Microbiology 2014-01-15 /pmc/articles/PMC3910181/ /pubmed/24516440 Text en Copyright © 2013, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC. |
spellingShingle | Research Paper Kaewklom, Siriporn Samosornsuk, Seksun Pipatsatitpong, Duangnate Aunpad, Ratchaneewan Colicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea |
title | Colicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea |
title_full | Colicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea |
title_fullStr | Colicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea |
title_full_unstemmed | Colicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea |
title_short | Colicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea |
title_sort | colicin type 7 produced by majority of shigella sonnei isolated from thai patients with diarrhoea |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910181/ https://www.ncbi.nlm.nih.gov/pubmed/24516440 |
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