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Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests

Although several invasive and noninvasive tests have been developed for the diagnosis of Helicobacter pylori infection, all of the tests have their limitations. We conducted a study to investigate and compare the suitability of rapid urease test (RUT), serology, histopathology and stool antigen test...

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Autores principales: Pourakbari, Babak, Ghazi, Mona, Mahmoudi, Shima, Mamishi, Setareh, Azhdarkosh, Hossein, Najafi, Mehri, Kazemi, Bahram, Salavati, Ali, Mirsalehian, Akbar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Microbiology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910191/
https://www.ncbi.nlm.nih.gov/pubmed/24516421
http://dx.doi.org/10.1590/S1517-83822013005000052
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author Pourakbari, Babak
Ghazi, Mona
Mahmoudi, Shima
Mamishi, Setareh
Azhdarkosh, Hossein
Najafi, Mehri
Kazemi, Bahram
Salavati, Ali
Mirsalehian, Akbar
author_facet Pourakbari, Babak
Ghazi, Mona
Mahmoudi, Shima
Mamishi, Setareh
Azhdarkosh, Hossein
Najafi, Mehri
Kazemi, Bahram
Salavati, Ali
Mirsalehian, Akbar
author_sort Pourakbari, Babak
collection PubMed
description Although several invasive and noninvasive tests have been developed for the diagnosis of Helicobacter pylori infection, all of the tests have their limitations. We conducted a study to investigate and compare the suitability of rapid urease test (RUT), serology, histopathology and stool antigen tests with polymerase chain reaction (PCR) for detection of H. pylori, and correlate the diagnostic methods with PCR. Eighty nine patients (61 adults, 28 children) referred to the Firoozgar Hospital and Children Medical Center Hospital for diagnostic upper gastrointestinal endoscopy entered to the study and noninvasive tests such as immunoassay for serological antibodies against H. pylori and detection of its antigen in feces were measured. The biopsies were utilized for histological examination, RUT and PCR. The H. pylori statuses were evaluated by the positivity of ureC PCR in biopsy specimens and 53 subjects had H. pylori positive result. Histopathology showed high overall performance in adults and children with sensitivity and specificity 100% and 90%, respectively. Sensitivity, specificity, and accuracy for stool antigen test were 87.8%, 75% and 82%, respectively. Correlation of RUT, serology (IgG), histopathology and stool antigen tests with PCR were 0.82, 0.32, 0.91 and 0.63, respectively. In conclusion, the RUT and histopathology are as accurate as the PCR of biopsy and stool antigen test can consider as appropriate noninvasive test for detection of H. pylori infection.
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spelling pubmed-39101912014-02-10 Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests Pourakbari, Babak Ghazi, Mona Mahmoudi, Shima Mamishi, Setareh Azhdarkosh, Hossein Najafi, Mehri Kazemi, Bahram Salavati, Ali Mirsalehian, Akbar Braz J Microbiol Research Paper Although several invasive and noninvasive tests have been developed for the diagnosis of Helicobacter pylori infection, all of the tests have their limitations. We conducted a study to investigate and compare the suitability of rapid urease test (RUT), serology, histopathology and stool antigen tests with polymerase chain reaction (PCR) for detection of H. pylori, and correlate the diagnostic methods with PCR. Eighty nine patients (61 adults, 28 children) referred to the Firoozgar Hospital and Children Medical Center Hospital for diagnostic upper gastrointestinal endoscopy entered to the study and noninvasive tests such as immunoassay for serological antibodies against H. pylori and detection of its antigen in feces were measured. The biopsies were utilized for histological examination, RUT and PCR. The H. pylori statuses were evaluated by the positivity of ureC PCR in biopsy specimens and 53 subjects had H. pylori positive result. Histopathology showed high overall performance in adults and children with sensitivity and specificity 100% and 90%, respectively. Sensitivity, specificity, and accuracy for stool antigen test were 87.8%, 75% and 82%, respectively. Correlation of RUT, serology (IgG), histopathology and stool antigen tests with PCR were 0.82, 0.32, 0.91 and 0.63, respectively. In conclusion, the RUT and histopathology are as accurate as the PCR of biopsy and stool antigen test can consider as appropriate noninvasive test for detection of H. pylori infection. Brazilian Society of Microbiology 2013-11-15 /pmc/articles/PMC3910191/ /pubmed/24516421 http://dx.doi.org/10.1590/S1517-83822013005000052 Text en Copyright © 2013, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Research Paper
Pourakbari, Babak
Ghazi, Mona
Mahmoudi, Shima
Mamishi, Setareh
Azhdarkosh, Hossein
Najafi, Mehri
Kazemi, Bahram
Salavati, Ali
Mirsalehian, Akbar
Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests
title Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests
title_full Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests
title_fullStr Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests
title_full_unstemmed Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests
title_short Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests
title_sort diagnosis of helicobacter pylori infection by invasive and noninvasive tests
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910191/
https://www.ncbi.nlm.nih.gov/pubmed/24516421
http://dx.doi.org/10.1590/S1517-83822013005000052
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