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Incidence and transferability of antibiotic resistance in the enteric bacteria isolated from hospital wastewater

This study reports the occurrence of antibiotic resistance and production of β-lactamases including extended spectrum beta-lactamases (ESβL) in enteric bacteria isolated from hospital wastewater. Among sixty-nine isolates, tested for antibiotic sensitivity, 73.9% strains were resistant to ampicillin...

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Autores principales: Alam, Mohammad Zubair, Aqil, Farrukh, Ahmad, Iqbal, Ahmad, Shamim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Microbiology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910192/
https://www.ncbi.nlm.nih.gov/pubmed/24516448
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author Alam, Mohammad Zubair
Aqil, Farrukh
Ahmad, Iqbal
Ahmad, Shamim
author_facet Alam, Mohammad Zubair
Aqil, Farrukh
Ahmad, Iqbal
Ahmad, Shamim
author_sort Alam, Mohammad Zubair
collection PubMed
description This study reports the occurrence of antibiotic resistance and production of β-lactamases including extended spectrum beta-lactamases (ESβL) in enteric bacteria isolated from hospital wastewater. Among sixty-nine isolates, tested for antibiotic sensitivity, 73.9% strains were resistant to ampicillin followed by nalidixic acid (72.5%), penicillin (63.8%), co-trimoxazole (55.1%), norfloxacin (53.6%), methicillin (52.7%), cefuroxime (39.1%), cefotaxime (23.2%) and cefixime (20.3%). Resistance to streptomycin, chloramphenicol, nitrofurantoin, tetracycline, and doxycycline was recorded in less than 13% of the strains. The minimum inhibitory concentration (MIC) showed a high level of resistance (800–1600 μg/mL) to one or more antibiotics. Sixty three (91%) isolates produced β-lactamases as determined by rapid iodometric test. Multiple antibiotic resistances were noted in both among ESβL and non-ESβL producers. The β-lactamases hydrolyzed multiple substrates including penicillin (78.8% isolates), ampicillin (62.3%), cefodroxil (52.2%), cefotoxime (21.7%) and cefuroxime (18.8%). Fifteen isolates producing ESβLs were found multidrug resistant. Four ESβL producing isolates could transfer their R-plasmid to the recipient strain E. coli K-12 with conjugation frequency ranging from 7.0 × 10(−3) to 8.8 × 10(−4). The findings indicated that ESβL producing enteric bacteria are common in the waste water. Such isolates may disseminate the multiple antibiotic resistance traits among bacterial community through genetic exchange mechanisms and thus requires immediate attention.
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spelling pubmed-39101922014-02-15 Incidence and transferability of antibiotic resistance in the enteric bacteria isolated from hospital wastewater Alam, Mohammad Zubair Aqil, Farrukh Ahmad, Iqbal Ahmad, Shamim Braz J Microbiol Research Paper This study reports the occurrence of antibiotic resistance and production of β-lactamases including extended spectrum beta-lactamases (ESβL) in enteric bacteria isolated from hospital wastewater. Among sixty-nine isolates, tested for antibiotic sensitivity, 73.9% strains were resistant to ampicillin followed by nalidixic acid (72.5%), penicillin (63.8%), co-trimoxazole (55.1%), norfloxacin (53.6%), methicillin (52.7%), cefuroxime (39.1%), cefotaxime (23.2%) and cefixime (20.3%). Resistance to streptomycin, chloramphenicol, nitrofurantoin, tetracycline, and doxycycline was recorded in less than 13% of the strains. The minimum inhibitory concentration (MIC) showed a high level of resistance (800–1600 μg/mL) to one or more antibiotics. Sixty three (91%) isolates produced β-lactamases as determined by rapid iodometric test. Multiple antibiotic resistances were noted in both among ESβL and non-ESβL producers. The β-lactamases hydrolyzed multiple substrates including penicillin (78.8% isolates), ampicillin (62.3%), cefodroxil (52.2%), cefotoxime (21.7%) and cefuroxime (18.8%). Fifteen isolates producing ESβLs were found multidrug resistant. Four ESβL producing isolates could transfer their R-plasmid to the recipient strain E. coli K-12 with conjugation frequency ranging from 7.0 × 10(−3) to 8.8 × 10(−4). The findings indicated that ESβL producing enteric bacteria are common in the waste water. Such isolates may disseminate the multiple antibiotic resistance traits among bacterial community through genetic exchange mechanisms and thus requires immediate attention. Brazilian Society of Microbiology 2014-01-15 /pmc/articles/PMC3910192/ /pubmed/24516448 Text en Copyright © 2013, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Research Paper
Alam, Mohammad Zubair
Aqil, Farrukh
Ahmad, Iqbal
Ahmad, Shamim
Incidence and transferability of antibiotic resistance in the enteric bacteria isolated from hospital wastewater
title Incidence and transferability of antibiotic resistance in the enteric bacteria isolated from hospital wastewater
title_full Incidence and transferability of antibiotic resistance in the enteric bacteria isolated from hospital wastewater
title_fullStr Incidence and transferability of antibiotic resistance in the enteric bacteria isolated from hospital wastewater
title_full_unstemmed Incidence and transferability of antibiotic resistance in the enteric bacteria isolated from hospital wastewater
title_short Incidence and transferability of antibiotic resistance in the enteric bacteria isolated from hospital wastewater
title_sort incidence and transferability of antibiotic resistance in the enteric bacteria isolated from hospital wastewater
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910192/
https://www.ncbi.nlm.nih.gov/pubmed/24516448
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