Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinica...

Descripción completa

Detalles Bibliográficos
Autores principales: Leal, C.A.G., Carvalho-Castro, G.A., Cottorello, A.C., Leite, R.C., Figueiredo, H.C.P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Microbiology 2013
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910209/
https://www.ncbi.nlm.nih.gov/pubmed/24516428
http://dx.doi.org/10.1590/S1517-83822013005000054
_version_ 1782301947883159552
author Leal, C.A.G.
Carvalho-Castro, G.A.
Cottorello, A.C.
Leite, R.C.
Figueiredo, H.C.P.
author_facet Leal, C.A.G.
Carvalho-Castro, G.A.
Cottorello, A.C.
Leite, R.C.
Figueiredo, H.C.P.
author_sort Leal, C.A.G.
collection PubMed
description The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
format Online
Article
Text
id pubmed-3910209
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Brazilian Society of Microbiology
record_format MEDLINE/PubMed
spelling pubmed-39102092014-02-10 Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD Leal, C.A.G. Carvalho-Castro, G.A. Cottorello, A.C. Leite, R.C. Figueiredo, H.C.P. Braz J Microbiol Short Communication The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples. Brazilian Society of Microbiology 2013-11-15 /pmc/articles/PMC3910209/ /pubmed/24516428 http://dx.doi.org/10.1590/S1517-83822013005000054 Text en Copyright © 2013, Sociedade Brasileira de Microbiologia
spellingShingle Short Communication
Leal, C.A.G.
Carvalho-Castro, G.A.
Cottorello, A.C.
Leite, R.C.
Figueiredo, H.C.P.
Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title_full Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title_fullStr Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title_full_unstemmed Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title_short Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title_sort comparative analysis of conventional pcr and real-time pcr to diagnose shrimp wsd
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910209/
https://www.ncbi.nlm.nih.gov/pubmed/24516428
http://dx.doi.org/10.1590/S1517-83822013005000054
work_keys_str_mv AT lealcag comparativeanalysisofconventionalpcrandrealtimepcrtodiagnoseshrimpwsd
AT carvalhocastroga comparativeanalysisofconventionalpcrandrealtimepcrtodiagnoseshrimpwsd
AT cottorelloac comparativeanalysisofconventionalpcrandrealtimepcrtodiagnoseshrimpwsd
AT leiterc comparativeanalysisofconventionalpcrandrealtimepcrtodiagnoseshrimpwsd
AT figueiredohcp comparativeanalysisofconventionalpcrandrealtimepcrtodiagnoseshrimpwsd

Ejemplares similares