Cargando…
Endophytic fungi producing of esterases: Evaluation in vitro of the enzymatic activity using pH indicator
A sensitive and efficient colorimetric method was optimized for detection of esterase enzymes produced by endophytic fungi for development of High-Throughput Screening (HTS). The fungi were isolated and obtained previously from plant species of Cerrado and Atlantic Forest located in areas of environ...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Brazilian Society of Microbiology
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910213/ https://www.ncbi.nlm.nih.gov/pubmed/24516461 |
_version_ | 1782301948824780800 |
---|---|
author | Lisboa, Helen Cristina Fávero Biasetto, Carolina Rabal de Medeiros, João Batista Araújo, Ângela Regina Silva, Dulce Helena Siqueira Teles, Helder Lopes Trevisan, Henrique Celso |
author_facet | Lisboa, Helen Cristina Fávero Biasetto, Carolina Rabal de Medeiros, João Batista Araújo, Ângela Regina Silva, Dulce Helena Siqueira Teles, Helder Lopes Trevisan, Henrique Celso |
author_sort | Lisboa, Helen Cristina Fávero |
collection | PubMed |
description | A sensitive and efficient colorimetric method was optimized for detection of esterase enzymes produced by endophytic fungi for development of High-Throughput Screening (HTS). The fungi were isolated and obtained previously from plant species of Cerrado and Atlantic Forest located in areas of environmental preservation in the State of Sao Paulo / Brazil, as part of the project “Chemical and biological prospecting endophytic fungi associated to plant species of Cerrado and Atlantic Forest”. The compounds ethyl butyrate, ethyl acetate and methyl propionate were used as standards esters which were hydrolyzed by extracellular enzyme from endophytic fungi (EC. 3.1.1.1 - carboxyl-esterases) for production of carboxylic acids. Thus, the reduction of the pH increases the protonated indicator concentration (bromothymol blue), changing the color of the reaction medium (from blue to yellow), that can be observed and measured by spectrophotometry at 616 nm. The methodology with acid-base indicator was performed on 13 microorganisms, aiming Periconia atropurpurea as a potential source of esterase for biotransformation of short chain esters. The results also evidenced that this methodology showed to be efficient, fast, cheap, having low consumption of reagents and easy development, and can be applied to screen carboxylic-ester hydrolases in a large number of microorganisms. |
format | Online Article Text |
id | pubmed-3910213 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Brazilian Society of Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-39102132014-02-10 Endophytic fungi producing of esterases: Evaluation in vitro of the enzymatic activity using pH indicator Lisboa, Helen Cristina Fávero Biasetto, Carolina Rabal de Medeiros, João Batista Araújo, Ângela Regina Silva, Dulce Helena Siqueira Teles, Helder Lopes Trevisan, Henrique Celso Braz J Microbiol Research Paper A sensitive and efficient colorimetric method was optimized for detection of esterase enzymes produced by endophytic fungi for development of High-Throughput Screening (HTS). The fungi were isolated and obtained previously from plant species of Cerrado and Atlantic Forest located in areas of environmental preservation in the State of Sao Paulo / Brazil, as part of the project “Chemical and biological prospecting endophytic fungi associated to plant species of Cerrado and Atlantic Forest”. The compounds ethyl butyrate, ethyl acetate and methyl propionate were used as standards esters which were hydrolyzed by extracellular enzyme from endophytic fungi (EC. 3.1.1.1 - carboxyl-esterases) for production of carboxylic acids. Thus, the reduction of the pH increases the protonated indicator concentration (bromothymol blue), changing the color of the reaction medium (from blue to yellow), that can be observed and measured by spectrophotometry at 616 nm. The methodology with acid-base indicator was performed on 13 microorganisms, aiming Periconia atropurpurea as a potential source of esterase for biotransformation of short chain esters. The results also evidenced that this methodology showed to be efficient, fast, cheap, having low consumption of reagents and easy development, and can be applied to screen carboxylic-ester hydrolases in a large number of microorganisms. Brazilian Society of Microbiology 2014-01-10 /pmc/articles/PMC3910213/ /pubmed/24516461 Text en Copyright © 2013, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC. |
spellingShingle | Research Paper Lisboa, Helen Cristina Fávero Biasetto, Carolina Rabal de Medeiros, João Batista Araújo, Ângela Regina Silva, Dulce Helena Siqueira Teles, Helder Lopes Trevisan, Henrique Celso Endophytic fungi producing of esterases: Evaluation in vitro of the enzymatic activity using pH indicator |
title | Endophytic fungi producing of esterases: Evaluation in vitro of the enzymatic activity using pH indicator |
title_full | Endophytic fungi producing of esterases: Evaluation in vitro of the enzymatic activity using pH indicator |
title_fullStr | Endophytic fungi producing of esterases: Evaluation in vitro of the enzymatic activity using pH indicator |
title_full_unstemmed | Endophytic fungi producing of esterases: Evaluation in vitro of the enzymatic activity using pH indicator |
title_short | Endophytic fungi producing of esterases: Evaluation in vitro of the enzymatic activity using pH indicator |
title_sort | endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using ph indicator |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910213/ https://www.ncbi.nlm.nih.gov/pubmed/24516461 |
work_keys_str_mv | AT lisboahelencristinafavero endophyticfungiproducingofesterasesevaluationinvitrooftheenzymaticactivityusingphindicator AT biasettocarolinarabal endophyticfungiproducingofesterasesevaluationinvitrooftheenzymaticactivityusingphindicator AT demedeirosjoaobatista endophyticfungiproducingofesterasesevaluationinvitrooftheenzymaticactivityusingphindicator AT araujoangelaregina endophyticfungiproducingofesterasesevaluationinvitrooftheenzymaticactivityusingphindicator AT silvadulcehelenasiqueira endophyticfungiproducingofesterasesevaluationinvitrooftheenzymaticactivityusingphindicator AT teleshelderlopes endophyticfungiproducingofesterasesevaluationinvitrooftheenzymaticactivityusingphindicator AT trevisanhenriquecelso endophyticfungiproducingofesterasesevaluationinvitrooftheenzymaticactivityusingphindicator |