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Endo- and exoglucanase activities in bacteria from mangrove sediment

The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus,...

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Autores principales: Júnior, Fábio Lino Soares, Dias, Armando Cavalcante Franco, Fasanella, Cristiane Cipola, Taketani, Rodrigo Gouvêa, de Souza Lima, André Oliveira, Melo, Itamar Soares, Andreote, Fernando Dini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Microbiology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910219/
https://www.ncbi.nlm.nih.gov/pubmed/24516466
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author Júnior, Fábio Lino Soares
Dias, Armando Cavalcante Franco
Fasanella, Cristiane Cipola
Taketani, Rodrigo Gouvêa
de Souza Lima, André Oliveira
Melo, Itamar Soares
Andreote, Fernando Dini
author_facet Júnior, Fábio Lino Soares
Dias, Armando Cavalcante Franco
Fasanella, Cristiane Cipola
Taketani, Rodrigo Gouvêa
de Souza Lima, André Oliveira
Melo, Itamar Soares
Andreote, Fernando Dini
author_sort Júnior, Fábio Lino Soares
collection PubMed
description The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from a mangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria, enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplemented with 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33 isolates) than exoglycolytic (19 isolates), and the degradation activity was shown to be modulated by the presence of NaCl. The isolated bacteria were clustered by BOX-PCR and further classified on the basis of partial 16S rRNA sequences as Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, Firmicutes or Bacteroidetes. Therefore, this study highlights the importance of studies focusing on the endemic species found in mangroves to exploit them as novel biotechnological tools for the degradation of cellulose.
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spelling pubmed-39102192014-02-15 Endo- and exoglucanase activities in bacteria from mangrove sediment Júnior, Fábio Lino Soares Dias, Armando Cavalcante Franco Fasanella, Cristiane Cipola Taketani, Rodrigo Gouvêa de Souza Lima, André Oliveira Melo, Itamar Soares Andreote, Fernando Dini Braz J Microbiol Research Paper The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from a mangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria, enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplemented with 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33 isolates) than exoglycolytic (19 isolates), and the degradation activity was shown to be modulated by the presence of NaCl. The isolated bacteria were clustered by BOX-PCR and further classified on the basis of partial 16S rRNA sequences as Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, Firmicutes or Bacteroidetes. Therefore, this study highlights the importance of studies focusing on the endemic species found in mangroves to exploit them as novel biotechnological tools for the degradation of cellulose. Brazilian Society of Microbiology 2014-01-15 /pmc/articles/PMC3910219/ /pubmed/24516466 Text en Copyright © 2013, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Research Paper
Júnior, Fábio Lino Soares
Dias, Armando Cavalcante Franco
Fasanella, Cristiane Cipola
Taketani, Rodrigo Gouvêa
de Souza Lima, André Oliveira
Melo, Itamar Soares
Andreote, Fernando Dini
Endo- and exoglucanase activities in bacteria from mangrove sediment
title Endo- and exoglucanase activities in bacteria from mangrove sediment
title_full Endo- and exoglucanase activities in bacteria from mangrove sediment
title_fullStr Endo- and exoglucanase activities in bacteria from mangrove sediment
title_full_unstemmed Endo- and exoglucanase activities in bacteria from mangrove sediment
title_short Endo- and exoglucanase activities in bacteria from mangrove sediment
title_sort endo- and exoglucanase activities in bacteria from mangrove sediment
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910219/
https://www.ncbi.nlm.nih.gov/pubmed/24516466
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