Cargando…

Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress

Propolis is a natural product widely used for humans. Due to its complex composition, a number of applications (antimicrobial, antiinflammatory, anesthetic, cytostatic and antioxidant) have been attributed to this substance. Using Saccharomyces cerevisiae as a eukaryotic model we investigated the me...

Descripción completa

Detalles Bibliográficos
Autores principales: de Sá, Rafael A., de Castro, Frederico A.V., Eleutherio, Elis C.A., de Souza, Raquel M., da Silva, Joaquim F.M., Pereira, Marcos D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Microbiology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910222/
https://www.ncbi.nlm.nih.gov/pubmed/24516431
http://dx.doi.org/10.1590/S1517-83822013005000062
_version_ 1782301950908301312
author de Sá, Rafael A.
de Castro, Frederico A.V.
Eleutherio, Elis C.A.
de Souza, Raquel M.
da Silva, Joaquim F.M.
Pereira, Marcos D.
author_facet de Sá, Rafael A.
de Castro, Frederico A.V.
Eleutherio, Elis C.A.
de Souza, Raquel M.
da Silva, Joaquim F.M.
Pereira, Marcos D.
author_sort de Sá, Rafael A.
collection PubMed
description Propolis is a natural product widely used for humans. Due to its complex composition, a number of applications (antimicrobial, antiinflammatory, anesthetic, cytostatic and antioxidant) have been attributed to this substance. Using Saccharomyces cerevisiae as a eukaryotic model we investigated the mechanisms underlying the antioxidant effect of propolis from Guarapari against oxidative stress. Submitting a wild type (BY4741) and antioxidant deficient strains (ctt1Δ, sod1Δ, gsh1Δ, gtt1Δ and gtt2Δ) either to 15 mM menadione or to 2 mM hydrogen peroxide during 60 min, we observed that all strains, except the mutant sod1Δ, acquired tolerance when previously treated with 25 μg/mL of alcoholic propolis extract. Such a treatment reduced the levels of ROS generation and of lipid peroxidation, after oxidative stress. The increase in Cu/Zn-Sod activity by propolis suggests that the protection might be acting synergistically with Cu/Zn-Sod.
format Online
Article
Text
id pubmed-3910222
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Brazilian Society of Microbiology
record_format MEDLINE/PubMed
spelling pubmed-39102222014-02-10 Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress de Sá, Rafael A. de Castro, Frederico A.V. Eleutherio, Elis C.A. de Souza, Raquel M. da Silva, Joaquim F.M. Pereira, Marcos D. Braz J Microbiol Research Paper Propolis is a natural product widely used for humans. Due to its complex composition, a number of applications (antimicrobial, antiinflammatory, anesthetic, cytostatic and antioxidant) have been attributed to this substance. Using Saccharomyces cerevisiae as a eukaryotic model we investigated the mechanisms underlying the antioxidant effect of propolis from Guarapari against oxidative stress. Submitting a wild type (BY4741) and antioxidant deficient strains (ctt1Δ, sod1Δ, gsh1Δ, gtt1Δ and gtt2Δ) either to 15 mM menadione or to 2 mM hydrogen peroxide during 60 min, we observed that all strains, except the mutant sod1Δ, acquired tolerance when previously treated with 25 μg/mL of alcoholic propolis extract. Such a treatment reduced the levels of ROS generation and of lipid peroxidation, after oxidative stress. The increase in Cu/Zn-Sod activity by propolis suggests that the protection might be acting synergistically with Cu/Zn-Sod. Brazilian Society of Microbiology 2013-12-17 /pmc/articles/PMC3910222/ /pubmed/24516431 http://dx.doi.org/10.1590/S1517-83822013005000062 Text en Copyright © 2013, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Research Paper
de Sá, Rafael A.
de Castro, Frederico A.V.
Eleutherio, Elis C.A.
de Souza, Raquel M.
da Silva, Joaquim F.M.
Pereira, Marcos D.
Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress
title Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress
title_full Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress
title_fullStr Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress
title_full_unstemmed Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress
title_short Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress
title_sort brazilian propolis protects saccharomyces cerevisiae cells against oxidative stress
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910222/
https://www.ncbi.nlm.nih.gov/pubmed/24516431
http://dx.doi.org/10.1590/S1517-83822013005000062
work_keys_str_mv AT desarafaela brazilianpropolisprotectssaccharomycescerevisiaecellsagainstoxidativestress
AT decastrofredericoav brazilianpropolisprotectssaccharomycescerevisiaecellsagainstoxidativestress
AT eleutherioelisca brazilianpropolisprotectssaccharomycescerevisiaecellsagainstoxidativestress
AT desouzaraquelm brazilianpropolisprotectssaccharomycescerevisiaecellsagainstoxidativestress
AT dasilvajoaquimfm brazilianpropolisprotectssaccharomycescerevisiaecellsagainstoxidativestress
AT pereiramarcosd brazilianpropolisprotectssaccharomycescerevisiaecellsagainstoxidativestress