RNA-guided gene activation by CRISPR-Cas9-based transcription factors

Technologies for engineering synthetic transcription factors have enabled many advances in medicine and science. In contrast to existing methods based on engineering of new DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Co-expre...

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Detalles Bibliográficos
Autores principales: Perez-Pinera, Pablo, Kocak, D. Dewran, Vockley, Christopher M., Adler, Andrew F., Kabadi, Ami M., Polstein, Lauren R., Thakore, Pratiksha I., Glass, Katherine A., Ousterout, David G., Leong, Kam W., Guilak, Farshid, Crawford, Gregory E., Reddy, Timothy E., Gersbach, Charles A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3911785/
https://www.ncbi.nlm.nih.gov/pubmed/23892895
http://dx.doi.org/10.1038/nmeth.2600
Descripción
Sumario:Technologies for engineering synthetic transcription factors have enabled many advances in medicine and science. In contrast to existing methods based on engineering of new DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Co-expression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.

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