RNA-guided gene activation by CRISPR-Cas9-based transcription factors

Technologies for engineering synthetic transcription factors have enabled many advances in medicine and science. In contrast to existing methods based on engineering of new DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Co-expre...

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Autores principales: Perez-Pinera, Pablo, Kocak, D. Dewran, Vockley, Christopher M., Adler, Andrew F., Kabadi, Ami M., Polstein, Lauren R., Thakore, Pratiksha I., Glass, Katherine A., Ousterout, David G., Leong, Kam W., Guilak, Farshid, Crawford, Gregory E., Reddy, Timothy E., Gersbach, Charles A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3911785/
https://www.ncbi.nlm.nih.gov/pubmed/23892895
http://dx.doi.org/10.1038/nmeth.2600
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author Perez-Pinera, Pablo
Kocak, D. Dewran
Vockley, Christopher M.
Adler, Andrew F.
Kabadi, Ami M.
Polstein, Lauren R.
Thakore, Pratiksha I.
Glass, Katherine A.
Ousterout, David G.
Leong, Kam W.
Guilak, Farshid
Crawford, Gregory E.
Reddy, Timothy E.
Gersbach, Charles A.
author_facet Perez-Pinera, Pablo
Kocak, D. Dewran
Vockley, Christopher M.
Adler, Andrew F.
Kabadi, Ami M.
Polstein, Lauren R.
Thakore, Pratiksha I.
Glass, Katherine A.
Ousterout, David G.
Leong, Kam W.
Guilak, Farshid
Crawford, Gregory E.
Reddy, Timothy E.
Gersbach, Charles A.
author_sort Perez-Pinera, Pablo
collection PubMed
description Technologies for engineering synthetic transcription factors have enabled many advances in medicine and science. In contrast to existing methods based on engineering of new DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Co-expression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.
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spelling pubmed-39117852014-04-01 RNA-guided gene activation by CRISPR-Cas9-based transcription factors Perez-Pinera, Pablo Kocak, D. Dewran Vockley, Christopher M. Adler, Andrew F. Kabadi, Ami M. Polstein, Lauren R. Thakore, Pratiksha I. Glass, Katherine A. Ousterout, David G. Leong, Kam W. Guilak, Farshid Crawford, Gregory E. Reddy, Timothy E. Gersbach, Charles A. Nat Methods Article Technologies for engineering synthetic transcription factors have enabled many advances in medicine and science. In contrast to existing methods based on engineering of new DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Co-expression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation. 2013-07-25 2013-10 /pmc/articles/PMC3911785/ /pubmed/23892895 http://dx.doi.org/10.1038/nmeth.2600 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Perez-Pinera, Pablo
Kocak, D. Dewran
Vockley, Christopher M.
Adler, Andrew F.
Kabadi, Ami M.
Polstein, Lauren R.
Thakore, Pratiksha I.
Glass, Katherine A.
Ousterout, David G.
Leong, Kam W.
Guilak, Farshid
Crawford, Gregory E.
Reddy, Timothy E.
Gersbach, Charles A.
RNA-guided gene activation by CRISPR-Cas9-based transcription factors
title RNA-guided gene activation by CRISPR-Cas9-based transcription factors
title_full RNA-guided gene activation by CRISPR-Cas9-based transcription factors
title_fullStr RNA-guided gene activation by CRISPR-Cas9-based transcription factors
title_full_unstemmed RNA-guided gene activation by CRISPR-Cas9-based transcription factors
title_short RNA-guided gene activation by CRISPR-Cas9-based transcription factors
title_sort rna-guided gene activation by crispr-cas9-based transcription factors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3911785/
https://www.ncbi.nlm.nih.gov/pubmed/23892895
http://dx.doi.org/10.1038/nmeth.2600
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