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High Degree of Overlap between Responses to a Virus and to the House Dust Mite Allergen in Airway Epithelial Cells
BACKGROUND: Airway epithelium is widely considered to play an active role in immune responses through its ability to detect changes in the environment and to generate a microenvironment for immune competent cells. Therefore, besides its role as a physical barrier, epithelium affects the outcome of t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912021/ https://www.ncbi.nlm.nih.gov/pubmed/24498371 http://dx.doi.org/10.1371/journal.pone.0087768 |
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author | Golebski, Korneliusz Luiten, Silvia van Egmond, Danielle de Groot, Esther Röschmann, Kristina Irene Lisolette Fokkens, Wytske Johanna van Drunen, Cornelis Maria |
author_facet | Golebski, Korneliusz Luiten, Silvia van Egmond, Danielle de Groot, Esther Röschmann, Kristina Irene Lisolette Fokkens, Wytske Johanna van Drunen, Cornelis Maria |
author_sort | Golebski, Korneliusz |
collection | PubMed |
description | BACKGROUND: Airway epithelium is widely considered to play an active role in immune responses through its ability to detect changes in the environment and to generate a microenvironment for immune competent cells. Therefore, besides its role as a physical barrier, epithelium affects the outcome of the immune response by the production of various pro-inflammatory mediators. METHODS: We stimulated airway epithelial cells with viral double stranded RNA analogue poly(I:C) or with house dust mite in a time course of 24 hours. In order to determine cytokines production by stimulated cells, we performed multiplex enzyme linked immunosorbant assay (ELISA). RESULTS: We demonstrate that the temporal pattern of the genes that respond to virus exposure in airway epithelium resembles to a significant degree their pattern of response to HDM. The gene expression pattern of EGR1, DUSP1, FOSL1, JUN, MYC, and IL6 is rather similar after viral (poly(I:C)) and HDM exposure. However, both triggers also induce a specific response (e.g. ATF3, FOS, and NFKB1). We confirmed these data by showing that epithelial cells produce a variety of similar mediators in response to both poly(I:C) and HDM challenge (IL1-RA, IL-17, IFN-α and MIP1-α), sometimes with a quantitative difference in response (IL2-R, IL-6, IL-8, MCP-1, MIG, and HGF). Interestingly, only four mediators (IL-12, IP-10, RANTES and VEGF) where up-regulated specifically by poly(I:C) and not by HDM. Additionally, we report that pre-exposure to HDM deregulates production of cytokines and mediators in response to poly(I:C). CONCLUSIONS: Epithelial cells responses to the HDM-allergen and a virus strongly resemble both in gene expression and in protein level explaining why these two responses may affect each other. |
format | Online Article Text |
id | pubmed-3912021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39120212014-02-04 High Degree of Overlap between Responses to a Virus and to the House Dust Mite Allergen in Airway Epithelial Cells Golebski, Korneliusz Luiten, Silvia van Egmond, Danielle de Groot, Esther Röschmann, Kristina Irene Lisolette Fokkens, Wytske Johanna van Drunen, Cornelis Maria PLoS One Research Article BACKGROUND: Airway epithelium is widely considered to play an active role in immune responses through its ability to detect changes in the environment and to generate a microenvironment for immune competent cells. Therefore, besides its role as a physical barrier, epithelium affects the outcome of the immune response by the production of various pro-inflammatory mediators. METHODS: We stimulated airway epithelial cells with viral double stranded RNA analogue poly(I:C) or with house dust mite in a time course of 24 hours. In order to determine cytokines production by stimulated cells, we performed multiplex enzyme linked immunosorbant assay (ELISA). RESULTS: We demonstrate that the temporal pattern of the genes that respond to virus exposure in airway epithelium resembles to a significant degree their pattern of response to HDM. The gene expression pattern of EGR1, DUSP1, FOSL1, JUN, MYC, and IL6 is rather similar after viral (poly(I:C)) and HDM exposure. However, both triggers also induce a specific response (e.g. ATF3, FOS, and NFKB1). We confirmed these data by showing that epithelial cells produce a variety of similar mediators in response to both poly(I:C) and HDM challenge (IL1-RA, IL-17, IFN-α and MIP1-α), sometimes with a quantitative difference in response (IL2-R, IL-6, IL-8, MCP-1, MIG, and HGF). Interestingly, only four mediators (IL-12, IP-10, RANTES and VEGF) where up-regulated specifically by poly(I:C) and not by HDM. Additionally, we report that pre-exposure to HDM deregulates production of cytokines and mediators in response to poly(I:C). CONCLUSIONS: Epithelial cells responses to the HDM-allergen and a virus strongly resemble both in gene expression and in protein level explaining why these two responses may affect each other. Public Library of Science 2014-02-03 /pmc/articles/PMC3912021/ /pubmed/24498371 http://dx.doi.org/10.1371/journal.pone.0087768 Text en © 2014 Golebski et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Golebski, Korneliusz Luiten, Silvia van Egmond, Danielle de Groot, Esther Röschmann, Kristina Irene Lisolette Fokkens, Wytske Johanna van Drunen, Cornelis Maria High Degree of Overlap between Responses to a Virus and to the House Dust Mite Allergen in Airway Epithelial Cells |
title | High Degree of Overlap between Responses to a Virus and to the House Dust Mite Allergen in Airway Epithelial Cells |
title_full | High Degree of Overlap between Responses to a Virus and to the House Dust Mite Allergen in Airway Epithelial Cells |
title_fullStr | High Degree of Overlap between Responses to a Virus and to the House Dust Mite Allergen in Airway Epithelial Cells |
title_full_unstemmed | High Degree of Overlap between Responses to a Virus and to the House Dust Mite Allergen in Airway Epithelial Cells |
title_short | High Degree of Overlap between Responses to a Virus and to the House Dust Mite Allergen in Airway Epithelial Cells |
title_sort | high degree of overlap between responses to a virus and to the house dust mite allergen in airway epithelial cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912021/ https://www.ncbi.nlm.nih.gov/pubmed/24498371 http://dx.doi.org/10.1371/journal.pone.0087768 |
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