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Effect of the systemic administration of methylprednisolone on the lungs of brain-dead donor rats undergoing pulmonary transplantation

OBJECTIVE: Most lung transplants are obtained from brain-dead donors. The physiopathology of brain death involves hemodynamics, the sympathetic nervous system, and inflammatory mechanisms. Administering methylprednisolone 60 min after inducing brain death in rats has been shown to modulate pulmonary...

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Autores principales: Araujo, Luiz Felipe Lopes, Holand, Arthur Rodrigo Ronconi, de Oliveira Paludo, Artur, Silva, Éverton Franco, Forgiarini, Luiz Alberto, Forgiarini, Luiz Felipe, Silva, Mariel Barbachan e, Andrade, Cristiano Feijó
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2014
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912341/
https://www.ncbi.nlm.nih.gov/pubmed/24519204
http://dx.doi.org/10.6061/clinics/2014(02)09
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author Araujo, Luiz Felipe Lopes
Holand, Arthur Rodrigo Ronconi
de Oliveira Paludo, Artur
Silva, Éverton Franco
Forgiarini, Luiz Alberto
Forgiarini, Luiz Felipe
Silva, Mariel Barbachan e
Andrade, Cristiano Feijó
author_facet Araujo, Luiz Felipe Lopes
Holand, Arthur Rodrigo Ronconi
de Oliveira Paludo, Artur
Silva, Éverton Franco
Forgiarini, Luiz Alberto
Forgiarini, Luiz Felipe
Silva, Mariel Barbachan e
Andrade, Cristiano Feijó
author_sort Araujo, Luiz Felipe Lopes
collection PubMed
description OBJECTIVE: Most lung transplants are obtained from brain-dead donors. The physiopathology of brain death involves hemodynamics, the sympathetic nervous system, and inflammatory mechanisms. Administering methylprednisolone 60 min after inducing brain death in rats has been shown to modulate pulmonary inflammatory activity. Our objective was to evaluate the effects of methylprednisolone on transplanted rat lungs from donors treated 60 min after brain death. METHODS: Twelve Wistar rats were anesthetized, and brain death was induced. They were randomly divided into two groups (n = 6), namely a control group, which was administered saline solution, and a methylprednisolone group, which received the drug 60 min after the induction of brain death. All of the animals were observed and ventilated for 2 h prior to being submitted to lung transplantation. We evaluated the hemodynamic and blood gas parameters, histological score, lung tissue levels of thiobarbituric acid-reactive substances, level of superoxide dismutase, level of tumor necrosis factor-alpha, and level of interleukin-1 beta. RESULTS: After transplantation, a significant reduction in the levels of tumor necrosis factor-alpha and IL-1β was observed in the group that received methylprednisolone (p = 0.0084 and p = 0.0155, respectively). There were no significant differences in tumor necrosis factor-alpha and superoxide dismutase levels between the control and methylprednisolone groups (p = 0.2644 and p = 0.7461, respectively). There were no significant differences in the blood gas parameters, hemodynamics, and histological alterations between the groups. CONCLUSION: The administration of methylprednisolone after brain death in donor rats reduces inflammatory activity in transplanted lungs but has no influence on parameters related to oxidative stress.
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spelling pubmed-39123412014-02-11 Effect of the systemic administration of methylprednisolone on the lungs of brain-dead donor rats undergoing pulmonary transplantation Araujo, Luiz Felipe Lopes Holand, Arthur Rodrigo Ronconi de Oliveira Paludo, Artur Silva, Éverton Franco Forgiarini, Luiz Alberto Forgiarini, Luiz Felipe Silva, Mariel Barbachan e Andrade, Cristiano Feijó Clinics (Sao Paulo) Basic Research OBJECTIVE: Most lung transplants are obtained from brain-dead donors. The physiopathology of brain death involves hemodynamics, the sympathetic nervous system, and inflammatory mechanisms. Administering methylprednisolone 60 min after inducing brain death in rats has been shown to modulate pulmonary inflammatory activity. Our objective was to evaluate the effects of methylprednisolone on transplanted rat lungs from donors treated 60 min after brain death. METHODS: Twelve Wistar rats were anesthetized, and brain death was induced. They were randomly divided into two groups (n = 6), namely a control group, which was administered saline solution, and a methylprednisolone group, which received the drug 60 min after the induction of brain death. All of the animals were observed and ventilated for 2 h prior to being submitted to lung transplantation. We evaluated the hemodynamic and blood gas parameters, histological score, lung tissue levels of thiobarbituric acid-reactive substances, level of superoxide dismutase, level of tumor necrosis factor-alpha, and level of interleukin-1 beta. RESULTS: After transplantation, a significant reduction in the levels of tumor necrosis factor-alpha and IL-1β was observed in the group that received methylprednisolone (p = 0.0084 and p = 0.0155, respectively). There were no significant differences in tumor necrosis factor-alpha and superoxide dismutase levels between the control and methylprednisolone groups (p = 0.2644 and p = 0.7461, respectively). There were no significant differences in the blood gas parameters, hemodynamics, and histological alterations between the groups. CONCLUSION: The administration of methylprednisolone after brain death in donor rats reduces inflammatory activity in transplanted lungs but has no influence on parameters related to oxidative stress. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2014-02 /pmc/articles/PMC3912341/ /pubmed/24519204 http://dx.doi.org/10.6061/clinics/2014(02)09 Text en Copyright © 2014 Hospital das Clínicas da FMUSP http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
Araujo, Luiz Felipe Lopes
Holand, Arthur Rodrigo Ronconi
de Oliveira Paludo, Artur
Silva, Éverton Franco
Forgiarini, Luiz Alberto
Forgiarini, Luiz Felipe
Silva, Mariel Barbachan e
Andrade, Cristiano Feijó
Effect of the systemic administration of methylprednisolone on the lungs of brain-dead donor rats undergoing pulmonary transplantation
title Effect of the systemic administration of methylprednisolone on the lungs of brain-dead donor rats undergoing pulmonary transplantation
title_full Effect of the systemic administration of methylprednisolone on the lungs of brain-dead donor rats undergoing pulmonary transplantation
title_fullStr Effect of the systemic administration of methylprednisolone on the lungs of brain-dead donor rats undergoing pulmonary transplantation
title_full_unstemmed Effect of the systemic administration of methylprednisolone on the lungs of brain-dead donor rats undergoing pulmonary transplantation
title_short Effect of the systemic administration of methylprednisolone on the lungs of brain-dead donor rats undergoing pulmonary transplantation
title_sort effect of the systemic administration of methylprednisolone on the lungs of brain-dead donor rats undergoing pulmonary transplantation
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912341/
https://www.ncbi.nlm.nih.gov/pubmed/24519204
http://dx.doi.org/10.6061/clinics/2014(02)09
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