Optimisation and Use of Humanised RBL NF-AT-GFP and NF-AT-DsRed Reporter Cell Lines Suitable for High-Throughput Scale Detection of Allergic Sensitisation in Array Format and Identification of the ECM–Integrin Interaction as Critical Factor

We have previously described a microarray platform combining live basophils with protein arrays suitable for high-throughput detection of sensitisation against allergens. During optimisation of this technique, we observed severe losses of adhering cells during the washing steps, particularly after a...

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Detalles Bibliográficos
Autores principales: Wang, Xiaowei, Cato, Paul, Lin, Hsiu-Chen, Li, Tongen, Wan, Daniel, Alcocer, Marcos J. C., Falcone, Franco H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912355/
https://www.ncbi.nlm.nih.gov/pubmed/23893250
http://dx.doi.org/10.1007/s12033-013-9689-x

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