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The APSES transcription factor Efg1 is a global regulator that controls morphogenesis and biofilm formation in Candida parapsilosis

Efg1 (a member of the APSES family) is an important regulator of hyphal growth and of the white-to-opaque transition in Candida albicans and very closely related species. We show that in Candida parapsilosis Efg1 is a major regulator of a different morphological switch at the colony level, from a co...

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Autores principales: Connolly, Leona A, Riccombeni, Alessandro, Grózer, Zsuzsana, Holland, Linda M, Lynch, Denise B, Andes, David R, Gácser, Attila, Butler, Geraldine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912905/
https://www.ncbi.nlm.nih.gov/pubmed/23895281
http://dx.doi.org/10.1111/mmi.12345
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author Connolly, Leona A
Riccombeni, Alessandro
Grózer, Zsuzsana
Holland, Linda M
Lynch, Denise B
Andes, David R
Gácser, Attila
Butler, Geraldine
author_facet Connolly, Leona A
Riccombeni, Alessandro
Grózer, Zsuzsana
Holland, Linda M
Lynch, Denise B
Andes, David R
Gácser, Attila
Butler, Geraldine
author_sort Connolly, Leona A
collection PubMed
description Efg1 (a member of the APSES family) is an important regulator of hyphal growth and of the white-to-opaque transition in Candida albicans and very closely related species. We show that in Candida parapsilosis Efg1 is a major regulator of a different morphological switch at the colony level, from a concentric to smooth morphology. The rate of switching is at least 20-fold increased in an efg1 knockout relative to wild type. Efg1 deletion strains also have reduced biofilm formation, attenuated virulence in an insect model, and increased sensitivity to SDS and caspofungin. Biofilm reduction is more dramatic in in vitro than in in vivo models. An Efg1 paralogue (Efh1) is restricted to Candida species, and does not regulate concentric-smooth phenotype switching, biofilm formation or stress response. We used ChIP-seq to identify the Efg1 regulon. A total of 931 promoter regions bound by Efg1 are highly enriched for transcription factors and regulatory proteins. Efg1 also binds to its own promoter, and negatively regulates its expression. Efg1 targets are enriched in binding sites for 93 additional transcription factors, including Ndt80. Our analysis suggests that Efg1 has an ancient role as regulator of development in fungi, and is central to several regulatory networks.
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spelling pubmed-39129052014-02-06 The APSES transcription factor Efg1 is a global regulator that controls morphogenesis and biofilm formation in Candida parapsilosis Connolly, Leona A Riccombeni, Alessandro Grózer, Zsuzsana Holland, Linda M Lynch, Denise B Andes, David R Gácser, Attila Butler, Geraldine Mol Microbiol Research Articles Efg1 (a member of the APSES family) is an important regulator of hyphal growth and of the white-to-opaque transition in Candida albicans and very closely related species. We show that in Candida parapsilosis Efg1 is a major regulator of a different morphological switch at the colony level, from a concentric to smooth morphology. The rate of switching is at least 20-fold increased in an efg1 knockout relative to wild type. Efg1 deletion strains also have reduced biofilm formation, attenuated virulence in an insect model, and increased sensitivity to SDS and caspofungin. Biofilm reduction is more dramatic in in vitro than in in vivo models. An Efg1 paralogue (Efh1) is restricted to Candida species, and does not regulate concentric-smooth phenotype switching, biofilm formation or stress response. We used ChIP-seq to identify the Efg1 regulon. A total of 931 promoter regions bound by Efg1 are highly enriched for transcription factors and regulatory proteins. Efg1 also binds to its own promoter, and negatively regulates its expression. Efg1 targets are enriched in binding sites for 93 additional transcription factors, including Ndt80. Our analysis suggests that Efg1 has an ancient role as regulator of development in fungi, and is central to several regulatory networks. John Wiley & Sons Ltd 2013-10 2013-08-15 /pmc/articles/PMC3912905/ /pubmed/23895281 http://dx.doi.org/10.1111/mmi.12345 Text en 2013 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Connolly, Leona A
Riccombeni, Alessandro
Grózer, Zsuzsana
Holland, Linda M
Lynch, Denise B
Andes, David R
Gácser, Attila
Butler, Geraldine
The APSES transcription factor Efg1 is a global regulator that controls morphogenesis and biofilm formation in Candida parapsilosis
title The APSES transcription factor Efg1 is a global regulator that controls morphogenesis and biofilm formation in Candida parapsilosis
title_full The APSES transcription factor Efg1 is a global regulator that controls morphogenesis and biofilm formation in Candida parapsilosis
title_fullStr The APSES transcription factor Efg1 is a global regulator that controls morphogenesis and biofilm formation in Candida parapsilosis
title_full_unstemmed The APSES transcription factor Efg1 is a global regulator that controls morphogenesis and biofilm formation in Candida parapsilosis
title_short The APSES transcription factor Efg1 is a global regulator that controls morphogenesis and biofilm formation in Candida parapsilosis
title_sort apses transcription factor efg1 is a global regulator that controls morphogenesis and biofilm formation in candida parapsilosis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912905/
https://www.ncbi.nlm.nih.gov/pubmed/23895281
http://dx.doi.org/10.1111/mmi.12345
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