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Increased IL-4 mRNA expression and poly-aromatic hydrocarbon concentrations from children with asthma

BACKGROUND: Asthma is the most common chronic childhood disease. Imbalance of cytokines released from T helper cells and environmental factors, such as exposure to poly-aromatic hydrocarbon (PAH), play pivotal roles in the pathogenesis of asthma. The aim of this study was to compare the mRNA express...

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Autores principales: Al-Daghri, Nasser M, Abd-Alrahman, Sherif, Draz, Hossam, Alkharfy, Khalid, Mohammed, Abdul Khader, Clerici, Mario S, Alokail, Majed S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912936/
https://www.ncbi.nlm.nih.gov/pubmed/24450480
http://dx.doi.org/10.1186/1471-2431-14-17
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author Al-Daghri, Nasser M
Abd-Alrahman, Sherif
Draz, Hossam
Alkharfy, Khalid
Mohammed, Abdul Khader
Clerici, Mario S
Alokail, Majed S
author_facet Al-Daghri, Nasser M
Abd-Alrahman, Sherif
Draz, Hossam
Alkharfy, Khalid
Mohammed, Abdul Khader
Clerici, Mario S
Alokail, Majed S
author_sort Al-Daghri, Nasser M
collection PubMed
description BACKGROUND: Asthma is the most common chronic childhood disease. Imbalance of cytokines released from T helper cells and environmental factors, such as exposure to poly-aromatic hydrocarbon (PAH), play pivotal roles in the pathogenesis of asthma. The aim of this study was to compare the mRNA expression patterns of Interleukin (IL)-4, interferon (IFN)-γ and Acyl Co A long chain 3 (ACSL3) in peripheral blood leukocytes of children with and without asthma. To correlate the obtained mRNA data with serum IL-4, IFN-γ and PAH levels. Further, to determine the effect of in vivo exposure to PAH on mRNA expression of IL-4, IFN-γ and ACSL3 genes in a rat model. METHODS: A total of 170 children below 16 years (85 pediatric asthma patients and 85 matched healthy controls) were randomly selected from the Riyadh Cohort, Saudi Arabia. Gene expression analysis was performed using qRTPCR. Serum IL-4, IFN-γ and PAH were measured using LINCOplex (human multiplex immunoassay kit) and HPLC respectively. RESULTS: IL-4 mRNA expression was significantly increased (P < 0.05) in children with asthma compared to healthy control group whereas no differences were observed for either IFN-γ or ACSL3 mRNA. Similarly, serum IL- 4 and PAHs concentration was significantly higher as well in children with asthma in whom IFN-γ was also significantly lower. Results obtained in rats showed that exposure to the benzopyrene prototype PAH resulted in a 2.6 fold (P < 0.001) increased IL-4 mRNA expression in blood. CONCLUSION: Peripheral blood IL-4 mRNA levels, serum concentration of this cytokine are elevated in children with asthma. Also, elevated levels of PAH were observed in children with asthma. Additionally, PAH administration in rodents resulted in an increased IL-4 mRNA which is supposed to partly mediate the inflammatory response noted in asthma.
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spelling pubmed-39129362014-02-05 Increased IL-4 mRNA expression and poly-aromatic hydrocarbon concentrations from children with asthma Al-Daghri, Nasser M Abd-Alrahman, Sherif Draz, Hossam Alkharfy, Khalid Mohammed, Abdul Khader Clerici, Mario S Alokail, Majed S BMC Pediatr Research Article BACKGROUND: Asthma is the most common chronic childhood disease. Imbalance of cytokines released from T helper cells and environmental factors, such as exposure to poly-aromatic hydrocarbon (PAH), play pivotal roles in the pathogenesis of asthma. The aim of this study was to compare the mRNA expression patterns of Interleukin (IL)-4, interferon (IFN)-γ and Acyl Co A long chain 3 (ACSL3) in peripheral blood leukocytes of children with and without asthma. To correlate the obtained mRNA data with serum IL-4, IFN-γ and PAH levels. Further, to determine the effect of in vivo exposure to PAH on mRNA expression of IL-4, IFN-γ and ACSL3 genes in a rat model. METHODS: A total of 170 children below 16 years (85 pediatric asthma patients and 85 matched healthy controls) were randomly selected from the Riyadh Cohort, Saudi Arabia. Gene expression analysis was performed using qRTPCR. Serum IL-4, IFN-γ and PAH were measured using LINCOplex (human multiplex immunoassay kit) and HPLC respectively. RESULTS: IL-4 mRNA expression was significantly increased (P < 0.05) in children with asthma compared to healthy control group whereas no differences were observed for either IFN-γ or ACSL3 mRNA. Similarly, serum IL- 4 and PAHs concentration was significantly higher as well in children with asthma in whom IFN-γ was also significantly lower. Results obtained in rats showed that exposure to the benzopyrene prototype PAH resulted in a 2.6 fold (P < 0.001) increased IL-4 mRNA expression in blood. CONCLUSION: Peripheral blood IL-4 mRNA levels, serum concentration of this cytokine are elevated in children with asthma. Also, elevated levels of PAH were observed in children with asthma. Additionally, PAH administration in rodents resulted in an increased IL-4 mRNA which is supposed to partly mediate the inflammatory response noted in asthma. BioMed Central 2014-01-23 /pmc/articles/PMC3912936/ /pubmed/24450480 http://dx.doi.org/10.1186/1471-2431-14-17 Text en Copyright © 2014 Al-Daghri et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Al-Daghri, Nasser M
Abd-Alrahman, Sherif
Draz, Hossam
Alkharfy, Khalid
Mohammed, Abdul Khader
Clerici, Mario S
Alokail, Majed S
Increased IL-4 mRNA expression and poly-aromatic hydrocarbon concentrations from children with asthma
title Increased IL-4 mRNA expression and poly-aromatic hydrocarbon concentrations from children with asthma
title_full Increased IL-4 mRNA expression and poly-aromatic hydrocarbon concentrations from children with asthma
title_fullStr Increased IL-4 mRNA expression and poly-aromatic hydrocarbon concentrations from children with asthma
title_full_unstemmed Increased IL-4 mRNA expression and poly-aromatic hydrocarbon concentrations from children with asthma
title_short Increased IL-4 mRNA expression and poly-aromatic hydrocarbon concentrations from children with asthma
title_sort increased il-4 mrna expression and poly-aromatic hydrocarbon concentrations from children with asthma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3912936/
https://www.ncbi.nlm.nih.gov/pubmed/24450480
http://dx.doi.org/10.1186/1471-2431-14-17
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