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Regulation of Chemokine CCL5 Synthesis in Human Peritoneal Fibroblasts: A Key Role of IFN-γ

Peritonitis is characterized by a coordinated influx of various leukocyte subpopulations. The pattern of leukocyte recruitment is controlled by chemokines secreted primarily by peritoneal mesothelial cells and macrophages. We have previously demonstrated that some chemokines may be also produced by...

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Autores principales: Kawka, Edyta, Witowski, Janusz, Fouqet, Nina, Tayama, Hironori, Bender, Thorsten O., Catar, Rusan, Dragun, Duska, Jörres, Achim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913084/
https://www.ncbi.nlm.nih.gov/pubmed/24523572
http://dx.doi.org/10.1155/2014/590654
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author Kawka, Edyta
Witowski, Janusz
Fouqet, Nina
Tayama, Hironori
Bender, Thorsten O.
Catar, Rusan
Dragun, Duska
Jörres, Achim
author_facet Kawka, Edyta
Witowski, Janusz
Fouqet, Nina
Tayama, Hironori
Bender, Thorsten O.
Catar, Rusan
Dragun, Duska
Jörres, Achim
author_sort Kawka, Edyta
collection PubMed
description Peritonitis is characterized by a coordinated influx of various leukocyte subpopulations. The pattern of leukocyte recruitment is controlled by chemokines secreted primarily by peritoneal mesothelial cells and macrophages. We have previously demonstrated that some chemokines may be also produced by human peritoneal fibroblasts (HPFB). Aim of our study was to assess the potential of HPFB in culture to release CCL5, a potent chemoattractant for mononuclear leukocytes. Quiescent HPFB released constitutively no or trace amounts of CCL5. Stimulation of HPFB with IL-1β and TNF-α resulted in a time- (up to 96 h) and dose-dependent increase in CCL5 expression and release. IFN-γ alone did not induce CCL5 secretion over a wide range of concentrations (0.01–100 U/mL). However, it synergistically amplified the effects of TNF-α and IL-1β through upregulation of CCL5 mRNA. Moreover, pretreatment of cells with IFN-γ upregulated CD40 receptor, which enabled HPFB to respond to a recombinant ligand of CD40 (CD40L). Exposure of IFN-γ-treated HPFB, but not of control cells, to CD40L resulted in a dose-dependent induction of CCL5. These data demonstrate that HPFB synthesise CCL5 in response to inflammatory mediators present in the inflamed peritoneal cavity. HPFB-derived CCL5 may thus contribute to the intraperitoneal recruitment of mononuclear leukocytes during peritonitis.
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spelling pubmed-39130842014-02-12 Regulation of Chemokine CCL5 Synthesis in Human Peritoneal Fibroblasts: A Key Role of IFN-γ Kawka, Edyta Witowski, Janusz Fouqet, Nina Tayama, Hironori Bender, Thorsten O. Catar, Rusan Dragun, Duska Jörres, Achim Mediators Inflamm Research Article Peritonitis is characterized by a coordinated influx of various leukocyte subpopulations. The pattern of leukocyte recruitment is controlled by chemokines secreted primarily by peritoneal mesothelial cells and macrophages. We have previously demonstrated that some chemokines may be also produced by human peritoneal fibroblasts (HPFB). Aim of our study was to assess the potential of HPFB in culture to release CCL5, a potent chemoattractant for mononuclear leukocytes. Quiescent HPFB released constitutively no or trace amounts of CCL5. Stimulation of HPFB with IL-1β and TNF-α resulted in a time- (up to 96 h) and dose-dependent increase in CCL5 expression and release. IFN-γ alone did not induce CCL5 secretion over a wide range of concentrations (0.01–100 U/mL). However, it synergistically amplified the effects of TNF-α and IL-1β through upregulation of CCL5 mRNA. Moreover, pretreatment of cells with IFN-γ upregulated CD40 receptor, which enabled HPFB to respond to a recombinant ligand of CD40 (CD40L). Exposure of IFN-γ-treated HPFB, but not of control cells, to CD40L resulted in a dose-dependent induction of CCL5. These data demonstrate that HPFB synthesise CCL5 in response to inflammatory mediators present in the inflamed peritoneal cavity. HPFB-derived CCL5 may thus contribute to the intraperitoneal recruitment of mononuclear leukocytes during peritonitis. Hindawi Publishing Corporation 2014 2014-01-09 /pmc/articles/PMC3913084/ /pubmed/24523572 http://dx.doi.org/10.1155/2014/590654 Text en Copyright © 2014 Edyta Kawka et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kawka, Edyta
Witowski, Janusz
Fouqet, Nina
Tayama, Hironori
Bender, Thorsten O.
Catar, Rusan
Dragun, Duska
Jörres, Achim
Regulation of Chemokine CCL5 Synthesis in Human Peritoneal Fibroblasts: A Key Role of IFN-γ
title Regulation of Chemokine CCL5 Synthesis in Human Peritoneal Fibroblasts: A Key Role of IFN-γ
title_full Regulation of Chemokine CCL5 Synthesis in Human Peritoneal Fibroblasts: A Key Role of IFN-γ
title_fullStr Regulation of Chemokine CCL5 Synthesis in Human Peritoneal Fibroblasts: A Key Role of IFN-γ
title_full_unstemmed Regulation of Chemokine CCL5 Synthesis in Human Peritoneal Fibroblasts: A Key Role of IFN-γ
title_short Regulation of Chemokine CCL5 Synthesis in Human Peritoneal Fibroblasts: A Key Role of IFN-γ
title_sort regulation of chemokine ccl5 synthesis in human peritoneal fibroblasts: a key role of ifn-γ
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913084/
https://www.ncbi.nlm.nih.gov/pubmed/24523572
http://dx.doi.org/10.1155/2014/590654
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