Expansion of CD133(+) Umbilical Cord Blood Derived Hematopoietic Stem Cells on a Biocompatible Microwells

Umbilical cord Blood (UCB) as a source of Hematopoietic Stem/Progenitor cells (HSPCs) used for Umbilical cord blood transplantation (UCBT). The main obstacle in application of this source as an appropriate source of HSPCs is low volume of this product. So ex vivo expansion of these cells in a microe...

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Detalles Bibliográficos
Autores principales: Soufizomorrod, Mina, Soleimani, Masoud, Hajifathali, Abbas, Mohammadi, Majid Mossahebi, Abroun, Saeed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913131/
https://www.ncbi.nlm.nih.gov/pubmed/24505514
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author Soufizomorrod, Mina
Soleimani, Masoud
Hajifathali, Abbas
Mohammadi, Majid Mossahebi
Abroun, Saeed
author_facet Soufizomorrod, Mina
Soleimani, Masoud
Hajifathali, Abbas
Mohammadi, Majid Mossahebi
Abroun, Saeed
author_sort Soufizomorrod, Mina
collection PubMed
description Umbilical cord Blood (UCB) as a source of Hematopoietic Stem/Progenitor cells (HSPCs) used for Umbilical cord blood transplantation (UCBT). The main obstacle in application of this source as an appropriate source of HSPCs is low volume of this product. So ex vivo expansion of these cells in a microenvironment which mimic body condition is important. In current study we designed biocompatible microwells in which collagene type I is coated by softlitography method. Our findings designated that in 3-Dimensional (3D) microenvironment CD133(+) UCB derived HSC expanded significantly compared to 2-Dimensional (2D) microenvironment.
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spelling pubmed-39131312014-02-06 Expansion of CD133(+) Umbilical Cord Blood Derived Hematopoietic Stem Cells on a Biocompatible Microwells Soufizomorrod, Mina Soleimani, Masoud Hajifathali, Abbas Mohammadi, Majid Mossahebi Abroun, Saeed Int J Hematol Oncol Stem Cell Res Original Article Umbilical cord Blood (UCB) as a source of Hematopoietic Stem/Progenitor cells (HSPCs) used for Umbilical cord blood transplantation (UCBT). The main obstacle in application of this source as an appropriate source of HSPCs is low volume of this product. So ex vivo expansion of these cells in a microenvironment which mimic body condition is important. In current study we designed biocompatible microwells in which collagene type I is coated by softlitography method. Our findings designated that in 3-Dimensional (3D) microenvironment CD133(+) UCB derived HSC expanded significantly compared to 2-Dimensional (2D) microenvironment. Tehran University of Medical Sciences 2013 /pmc/articles/PMC3913131/ /pubmed/24505514 Text en © 2013 Hematology-Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Soufizomorrod, Mina
Soleimani, Masoud
Hajifathali, Abbas
Mohammadi, Majid Mossahebi
Abroun, Saeed
Expansion of CD133(+) Umbilical Cord Blood Derived Hematopoietic Stem Cells on a Biocompatible Microwells
title Expansion of CD133(+) Umbilical Cord Blood Derived Hematopoietic Stem Cells on a Biocompatible Microwells
title_full Expansion of CD133(+) Umbilical Cord Blood Derived Hematopoietic Stem Cells on a Biocompatible Microwells
title_fullStr Expansion of CD133(+) Umbilical Cord Blood Derived Hematopoietic Stem Cells on a Biocompatible Microwells
title_full_unstemmed Expansion of CD133(+) Umbilical Cord Blood Derived Hematopoietic Stem Cells on a Biocompatible Microwells
title_short Expansion of CD133(+) Umbilical Cord Blood Derived Hematopoietic Stem Cells on a Biocompatible Microwells
title_sort expansion of cd133(+) umbilical cord blood derived hematopoietic stem cells on a biocompatible microwells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913131/
https://www.ncbi.nlm.nih.gov/pubmed/24505514
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