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Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba
BACKGROUND: The Antarctic krill, Euphausia superba is a pelagic crustacean, abundant in high-density swarms (10 000 – 30 000 ind/m(2)) with a circumpolar distribution and a key role in the food web of the Southern Ocean. Only three EST derived microsatellite markers have been used in previous geneti...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913320/ https://www.ncbi.nlm.nih.gov/pubmed/24490686 http://dx.doi.org/10.1186/1756-0500-7-73 |
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author | Candeias, Rui Teixeira, Sara Duarte, Carlos M Pearson, Gareth A |
author_facet | Candeias, Rui Teixeira, Sara Duarte, Carlos M Pearson, Gareth A |
author_sort | Candeias, Rui |
collection | PubMed |
description | BACKGROUND: The Antarctic krill, Euphausia superba is a pelagic crustacean, abundant in high-density swarms (10 000 – 30 000 ind/m(2)) with a circumpolar distribution and a key role in the food web of the Southern Ocean. Only three EST derived microsatellite markers have been used in previous genetic studies, hence we developed additional highly polymorphic microsatellite markers to allow robust studies of the genetic variability and population differentiation within this species. FINDINGS: The microsatellite markers described here were obtained through an enriched genomic library, followed by 454 pyrosequencing. A total of 10 microsatellite markers were tested in 32 individuals from the Antarctic Peninsula. One of the tested loci was fixed for one allele while the other was variable. Of the remaining nine markers, seven showed no departure from Hardy-Weinberg equilibrium. The mean number of alleles was 14.9. CONCLUSIONS: These markers open perspectives for population genetic studies of this species to unravel genetic structure, dispersal and population biology, vital information for future conservation. |
format | Online Article Text |
id | pubmed-3913320 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-39133202014-02-05 Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba Candeias, Rui Teixeira, Sara Duarte, Carlos M Pearson, Gareth A BMC Res Notes Short Report BACKGROUND: The Antarctic krill, Euphausia superba is a pelagic crustacean, abundant in high-density swarms (10 000 – 30 000 ind/m(2)) with a circumpolar distribution and a key role in the food web of the Southern Ocean. Only three EST derived microsatellite markers have been used in previous genetic studies, hence we developed additional highly polymorphic microsatellite markers to allow robust studies of the genetic variability and population differentiation within this species. FINDINGS: The microsatellite markers described here were obtained through an enriched genomic library, followed by 454 pyrosequencing. A total of 10 microsatellite markers were tested in 32 individuals from the Antarctic Peninsula. One of the tested loci was fixed for one allele while the other was variable. Of the remaining nine markers, seven showed no departure from Hardy-Weinberg equilibrium. The mean number of alleles was 14.9. CONCLUSIONS: These markers open perspectives for population genetic studies of this species to unravel genetic structure, dispersal and population biology, vital information for future conservation. BioMed Central 2014-02-03 /pmc/articles/PMC3913320/ /pubmed/24490686 http://dx.doi.org/10.1186/1756-0500-7-73 Text en Copyright © 2014 Candeias et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Short Report Candeias, Rui Teixeira, Sara Duarte, Carlos M Pearson, Gareth A Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba |
title | Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba |
title_full | Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba |
title_fullStr | Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba |
title_full_unstemmed | Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba |
title_short | Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba |
title_sort | characterization of polymorphic microsatellite loci in the antarctic krill euphausia superba |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913320/ https://www.ncbi.nlm.nih.gov/pubmed/24490686 http://dx.doi.org/10.1186/1756-0500-7-73 |
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