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Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba

BACKGROUND: The Antarctic krill, Euphausia superba is a pelagic crustacean, abundant in high-density swarms (10 000 – 30 000 ind/m(2)) with a circumpolar distribution and a key role in the food web of the Southern Ocean. Only three EST derived microsatellite markers have been used in previous geneti...

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Autores principales: Candeias, Rui, Teixeira, Sara, Duarte, Carlos M, Pearson, Gareth A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913320/
https://www.ncbi.nlm.nih.gov/pubmed/24490686
http://dx.doi.org/10.1186/1756-0500-7-73
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author Candeias, Rui
Teixeira, Sara
Duarte, Carlos M
Pearson, Gareth A
author_facet Candeias, Rui
Teixeira, Sara
Duarte, Carlos M
Pearson, Gareth A
author_sort Candeias, Rui
collection PubMed
description BACKGROUND: The Antarctic krill, Euphausia superba is a pelagic crustacean, abundant in high-density swarms (10 000 – 30 000 ind/m(2)) with a circumpolar distribution and a key role in the food web of the Southern Ocean. Only three EST derived microsatellite markers have been used in previous genetic studies, hence we developed additional highly polymorphic microsatellite markers to allow robust studies of the genetic variability and population differentiation within this species. FINDINGS: The microsatellite markers described here were obtained through an enriched genomic library, followed by 454 pyrosequencing. A total of 10 microsatellite markers were tested in 32 individuals from the Antarctic Peninsula. One of the tested loci was fixed for one allele while the other was variable. Of the remaining nine markers, seven showed no departure from Hardy-Weinberg equilibrium. The mean number of alleles was 14.9. CONCLUSIONS: These markers open perspectives for population genetic studies of this species to unravel genetic structure, dispersal and population biology, vital information for future conservation.
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spelling pubmed-39133202014-02-05 Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba Candeias, Rui Teixeira, Sara Duarte, Carlos M Pearson, Gareth A BMC Res Notes Short Report BACKGROUND: The Antarctic krill, Euphausia superba is a pelagic crustacean, abundant in high-density swarms (10 000 – 30 000 ind/m(2)) with a circumpolar distribution and a key role in the food web of the Southern Ocean. Only three EST derived microsatellite markers have been used in previous genetic studies, hence we developed additional highly polymorphic microsatellite markers to allow robust studies of the genetic variability and population differentiation within this species. FINDINGS: The microsatellite markers described here were obtained through an enriched genomic library, followed by 454 pyrosequencing. A total of 10 microsatellite markers were tested in 32 individuals from the Antarctic Peninsula. One of the tested loci was fixed for one allele while the other was variable. Of the remaining nine markers, seven showed no departure from Hardy-Weinberg equilibrium. The mean number of alleles was 14.9. CONCLUSIONS: These markers open perspectives for population genetic studies of this species to unravel genetic structure, dispersal and population biology, vital information for future conservation. BioMed Central 2014-02-03 /pmc/articles/PMC3913320/ /pubmed/24490686 http://dx.doi.org/10.1186/1756-0500-7-73 Text en Copyright © 2014 Candeias et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Candeias, Rui
Teixeira, Sara
Duarte, Carlos M
Pearson, Gareth A
Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba
title Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba
title_full Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba
title_fullStr Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba
title_full_unstemmed Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba
title_short Characterization of polymorphic microsatellite loci in the Antarctic krill Euphausia superba
title_sort characterization of polymorphic microsatellite loci in the antarctic krill euphausia superba
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913320/
https://www.ncbi.nlm.nih.gov/pubmed/24490686
http://dx.doi.org/10.1186/1756-0500-7-73
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