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Strategies Affording Prevascularized Cell-Based Constructs for Myocardial Tissue Engineering

The production of a functional cardiac tissue to be transplanted in the injured area of the infarcted myocardium represents a challenge for regenerative medicine. Most cell-based grafts are unviable because of inadequate perfusion; therefore, prevascularization might be a suitable approach for myoca...

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Autores principales: Muscari, Claudio, Giordano, Emanuele, Bonafè, Francesca, Govoni, Marco, Guarnieri, Carlo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913389/
https://www.ncbi.nlm.nih.gov/pubmed/24511317
http://dx.doi.org/10.1155/2014/434169
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author Muscari, Claudio
Giordano, Emanuele
Bonafè, Francesca
Govoni, Marco
Guarnieri, Carlo
author_facet Muscari, Claudio
Giordano, Emanuele
Bonafè, Francesca
Govoni, Marco
Guarnieri, Carlo
author_sort Muscari, Claudio
collection PubMed
description The production of a functional cardiac tissue to be transplanted in the injured area of the infarcted myocardium represents a challenge for regenerative medicine. Most cell-based grafts are unviable because of inadequate perfusion; therefore, prevascularization might be a suitable approach for myocardial tissue engineering. To this aim, cells with a differentiation potential towards vascular and cardiac muscle phenotypes have been cocultured in 2D or 3D appropriate scaffolds. In addition to these basic approaches, more sophisticated strategies have been followed employing mixed-cell sheets, microvascular modules, and inosculation from vascular explants. Technologies exerting spatial control of vascular cells, such as topographical surface roughening and ordered patterning, represent other ways to drive scaffold vascularization. Finally, microfluidic devices and bioreactors exerting mechanical stress have also been employed for high-throughput scaling-up production in order to accelerate muscle differentiation and speeding the endothelialization process. Future research should address issues such as how to optimize cells, biomaterials, and biochemical components to improve the vascular integration of the construct within the cardiac wall, satisfying the metabolic and functional needs of the myocardial tissue.
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spelling pubmed-39133892014-02-09 Strategies Affording Prevascularized Cell-Based Constructs for Myocardial Tissue Engineering Muscari, Claudio Giordano, Emanuele Bonafè, Francesca Govoni, Marco Guarnieri, Carlo Stem Cells Int Review Article The production of a functional cardiac tissue to be transplanted in the injured area of the infarcted myocardium represents a challenge for regenerative medicine. Most cell-based grafts are unviable because of inadequate perfusion; therefore, prevascularization might be a suitable approach for myocardial tissue engineering. To this aim, cells with a differentiation potential towards vascular and cardiac muscle phenotypes have been cocultured in 2D or 3D appropriate scaffolds. In addition to these basic approaches, more sophisticated strategies have been followed employing mixed-cell sheets, microvascular modules, and inosculation from vascular explants. Technologies exerting spatial control of vascular cells, such as topographical surface roughening and ordered patterning, represent other ways to drive scaffold vascularization. Finally, microfluidic devices and bioreactors exerting mechanical stress have also been employed for high-throughput scaling-up production in order to accelerate muscle differentiation and speeding the endothelialization process. Future research should address issues such as how to optimize cells, biomaterials, and biochemical components to improve the vascular integration of the construct within the cardiac wall, satisfying the metabolic and functional needs of the myocardial tissue. Hindawi Publishing Corporation 2014 2014-01-05 /pmc/articles/PMC3913389/ /pubmed/24511317 http://dx.doi.org/10.1155/2014/434169 Text en Copyright © 2014 Claudio Muscari et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Muscari, Claudio
Giordano, Emanuele
Bonafè, Francesca
Govoni, Marco
Guarnieri, Carlo
Strategies Affording Prevascularized Cell-Based Constructs for Myocardial Tissue Engineering
title Strategies Affording Prevascularized Cell-Based Constructs for Myocardial Tissue Engineering
title_full Strategies Affording Prevascularized Cell-Based Constructs for Myocardial Tissue Engineering
title_fullStr Strategies Affording Prevascularized Cell-Based Constructs for Myocardial Tissue Engineering
title_full_unstemmed Strategies Affording Prevascularized Cell-Based Constructs for Myocardial Tissue Engineering
title_short Strategies Affording Prevascularized Cell-Based Constructs for Myocardial Tissue Engineering
title_sort strategies affording prevascularized cell-based constructs for myocardial tissue engineering
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913389/
https://www.ncbi.nlm.nih.gov/pubmed/24511317
http://dx.doi.org/10.1155/2014/434169
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