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L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway

Rat studies demonstrated that type II diabetes mellitus (T2DM) decreases both the production and bioavailability of nitric oxide (NO). L-arginine (LA) provides the precursor for the production of NO. We hypothesized that LA dietary supplementation will preserve NO production via endothelial nitric o...

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Autores principales: Claybaugh, Taylor, Decker, Sarah, McCall, Kelly, Slyvka, Yuriy, Steimle, Jerrod, Wood, Aaron, Schaefer, Megan, Thuma, Jean, Inman, Sharon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913451/
https://www.ncbi.nlm.nih.gov/pubmed/24523733
http://dx.doi.org/10.1155/2014/171546
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author Claybaugh, Taylor
Decker, Sarah
McCall, Kelly
Slyvka, Yuriy
Steimle, Jerrod
Wood, Aaron
Schaefer, Megan
Thuma, Jean
Inman, Sharon
author_facet Claybaugh, Taylor
Decker, Sarah
McCall, Kelly
Slyvka, Yuriy
Steimle, Jerrod
Wood, Aaron
Schaefer, Megan
Thuma, Jean
Inman, Sharon
author_sort Claybaugh, Taylor
collection PubMed
description Rat studies demonstrated that type II diabetes mellitus (T2DM) decreases both the production and bioavailability of nitric oxide (NO). L-arginine (LA) provides the precursor for the production of NO. We hypothesized that LA dietary supplementation will preserve NO production via endothelial nitric oxide synthase (eNOS) causing renal microvascular vasodilation and increased glomerular blood flow and thus increasing glomerular filtration rate (GFR). This would impede the formation of reactive oxygen species which contributes to cell damage and death. LA supplementation preserved GFR in the treated diabetic rats compared to untreated diabetic rats. We provide evidence that this effect may be due to increased levels of eNOS and urinary cyclic guanosine monophosphate, which leads to renal microvascular vasodilation. Plasma nitrotyrosine was decreased in the LA treated rats; however, plasma nitrite levels remained unaffected as expected. Marked improvements in glucose tolerance were also observed in the LA treated diabetic rats. These results demonstrate that LA supplementation preserves NO activity and may delay the onset of insulin resistance and renal dysfunction during hyperglycemic stress. These results suggest the importance of the NO pathway in consequent renal dysfunction and in the development of insulin resistance in diabetic rats.
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spelling pubmed-39134512014-02-12 L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway Claybaugh, Taylor Decker, Sarah McCall, Kelly Slyvka, Yuriy Steimle, Jerrod Wood, Aaron Schaefer, Megan Thuma, Jean Inman, Sharon Int J Endocrinol Research Article Rat studies demonstrated that type II diabetes mellitus (T2DM) decreases both the production and bioavailability of nitric oxide (NO). L-arginine (LA) provides the precursor for the production of NO. We hypothesized that LA dietary supplementation will preserve NO production via endothelial nitric oxide synthase (eNOS) causing renal microvascular vasodilation and increased glomerular blood flow and thus increasing glomerular filtration rate (GFR). This would impede the formation of reactive oxygen species which contributes to cell damage and death. LA supplementation preserved GFR in the treated diabetic rats compared to untreated diabetic rats. We provide evidence that this effect may be due to increased levels of eNOS and urinary cyclic guanosine monophosphate, which leads to renal microvascular vasodilation. Plasma nitrotyrosine was decreased in the LA treated rats; however, plasma nitrite levels remained unaffected as expected. Marked improvements in glucose tolerance were also observed in the LA treated diabetic rats. These results demonstrate that LA supplementation preserves NO activity and may delay the onset of insulin resistance and renal dysfunction during hyperglycemic stress. These results suggest the importance of the NO pathway in consequent renal dysfunction and in the development of insulin resistance in diabetic rats. Hindawi Publishing Corporation 2014 2014-01-12 /pmc/articles/PMC3913451/ /pubmed/24523733 http://dx.doi.org/10.1155/2014/171546 Text en Copyright © 2014 Taylor Claybaugh et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Claybaugh, Taylor
Decker, Sarah
McCall, Kelly
Slyvka, Yuriy
Steimle, Jerrod
Wood, Aaron
Schaefer, Megan
Thuma, Jean
Inman, Sharon
L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway
title L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway
title_full L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway
title_fullStr L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway
title_full_unstemmed L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway
title_short L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway
title_sort l-arginine supplementation in type ii diabetic rats preserves renal function and improves insulin sensitivity by altering the nitric oxide pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913451/
https://www.ncbi.nlm.nih.gov/pubmed/24523733
http://dx.doi.org/10.1155/2014/171546
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