Choose Your Label Wisely: Water-Soluble Fluorophores Often Interact with Lipid Bilayers

Water-soluble organic fluorophores are widely used as labels in biological systems. However, in many cases these fluorophores can interact strongly with lipid bilayers, influencing the interaction of the target with the bilayer and/or leading to misleading fluorescent signals. Here, we quantify the...

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Detalles Bibliográficos
Autores principales: Hughes, Laura D., Rawle, Robert J., Boxer, Steven G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913624/
https://www.ncbi.nlm.nih.gov/pubmed/24503716
http://dx.doi.org/10.1371/journal.pone.0087649
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author Hughes, Laura D.
Rawle, Robert J.
Boxer, Steven G.
author_facet Hughes, Laura D.
Rawle, Robert J.
Boxer, Steven G.
author_sort Hughes, Laura D.
collection PubMed
description Water-soluble organic fluorophores are widely used as labels in biological systems. However, in many cases these fluorophores can interact strongly with lipid bilayers, influencing the interaction of the target with the bilayer and/or leading to misleading fluorescent signals. Here, we quantify the interaction of 32 common water-soluble dyes with model lipid bilayers to serve as an additional criterion when selecting a dye label.
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spelling pubmed-39136242014-02-06 Choose Your Label Wisely: Water-Soluble Fluorophores Often Interact with Lipid Bilayers Hughes, Laura D. Rawle, Robert J. Boxer, Steven G. PLoS One Research Article Water-soluble organic fluorophores are widely used as labels in biological systems. However, in many cases these fluorophores can interact strongly with lipid bilayers, influencing the interaction of the target with the bilayer and/or leading to misleading fluorescent signals. Here, we quantify the interaction of 32 common water-soluble dyes with model lipid bilayers to serve as an additional criterion when selecting a dye label. Public Library of Science 2014-02-04 /pmc/articles/PMC3913624/ /pubmed/24503716 http://dx.doi.org/10.1371/journal.pone.0087649 Text en © 2014 Hughes et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hughes, Laura D.
Rawle, Robert J.
Boxer, Steven G.
Choose Your Label Wisely: Water-Soluble Fluorophores Often Interact with Lipid Bilayers
title Choose Your Label Wisely: Water-Soluble Fluorophores Often Interact with Lipid Bilayers
title_full Choose Your Label Wisely: Water-Soluble Fluorophores Often Interact with Lipid Bilayers
title_fullStr Choose Your Label Wisely: Water-Soluble Fluorophores Often Interact with Lipid Bilayers
title_full_unstemmed Choose Your Label Wisely: Water-Soluble Fluorophores Often Interact with Lipid Bilayers
title_short Choose Your Label Wisely: Water-Soluble Fluorophores Often Interact with Lipid Bilayers
title_sort choose your label wisely: water-soluble fluorophores often interact with lipid bilayers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3913624/
https://www.ncbi.nlm.nih.gov/pubmed/24503716
http://dx.doi.org/10.1371/journal.pone.0087649
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